Detection of circulation tumor cells from peripheral blood in patients with urothelial carcinoma
Keywords:Circulatory tumor cells, urothelial cancer
Objective: The study was designed to detect CTC in bladder and urothelial cancer patients and find the association of CTC with the staging and grading of urothelial cancer.
Material and Method: Peripheral blood of bladder cancer patients who underwent operations from 2014 to 2015 was collected before the operations. Detection of circulating tumor cells used the quantitative reverse transcription polymerase chain reaction (qRT-PCR) method, using cytokeratin 20 (CK20) (GenBank accession number X73501).
Result: Twenty-seven patients were enrolled in the study; the CK20 gene was detected in every patient’s peripheral blood. CTC was higher in >T2 stage compared to the lower stages, but not significantly (0.0056 vs 0.0107, p-value 0.057). Tumor patients who had a high-grade tumor had a higher CTC in their blood significantly compared to patients with low-grade tumors (0.0087 vs 0.0025, p-value 0.006). There was no significant difference in CTC when comparing gender and urine cytology.
Conclusion: CTC is correlated with bladder cancer. CTC of patients with high-grade tumors was found to be significantly higher than in the lower-grade group.
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