TY - JOUR AU - Payomthip , Natnicha AU - Panduang, Thamonwan AU - Aimsan, Warinthorn AU - Chakritbudsabong, Warunya AU - Chanapiwat , Panida AU - Kaeoket , Kampon AU - Rungarunlert, Sasitorn PY - 2022/09/09 Y2 - 2024/03/29 TI - A Preliminary In Vitro Study on the Effect of Zika Virus Strains on the Quality of Boar Sperm JF - Journal of Applied Animal Science JA - J Appl Anim Sci VL - 15 IS - 1 SE - Research Articles DO - UR - https://he02.tci-thaijo.org/index.php/jaas_muvs/article/view/258582 SP - 35-46 AB - <p>A million people in various countries have been infected by the Zika virus (ZIKV). Sexual transmission is one of the human infectious transmissions. ZIKV causes neurological disease and microcephaly in infants whose mothers have been infected. Domestic pigs were used as an experimental model because the symptoms of ZIKV-infected piglets are similar to humans. Nowadays, minimal evidence of ZIKV infection in boar semen has been published, and there is no data on the effect of ZIKV strains. Thus, this study compares the quality of boar semen infected by different ZIKV strains. The fresh semen from two boars were collected by gloved-hand technique. The quality of the semen was determined and it was separated into three groups: a non-infected semen (mock) group, and two ZIKV-infected semen groups (strains SV0010/15, and SV0127/14).  Then, at 2, 24, 48, and 72 hours after infection, the sperm viability and acrosomal integrity were assessed using Eosin-nigrosin and FITC-PNA, respectively. The morphology of head and tail sperms was determined using William’s stain and formal saline, respectively. The results indicated that there were no significant differences in the quality of boar semen between groups (<em>P</em> &gt; 0.05), but that sperm head and tail abnormalities trended to be increased overtime in all groups. In conclusion, the boar sperm may be not a target cell of ZIKV. Thus, additional samples for further research on the ZIKV receptor in sperm should be acquired.</p> ER -