Ramathibodi Medical Journal https://he02.tci-thaijo.org/index.php/ramajournal <p>Ramathibodi Medical Journal (RMJ) publishes article on clinical and laboratory medicine, health care policy and economics, medical education and ethics, and related topics. We welcome submissions from authors worldwide.</p> <p>ISSN 0125-3611 (Print)&nbsp;</p> <p>ISSN 2651-0561 (Online)</p> <p>Indexed in:&nbsp;&nbsp;<strong>&nbsp;&nbsp;<a title="ASEAN CITATION INDEX" href="https://www.asean-cites.org/" target="_blank" rel="noopener"><img src="/public/site/images/nuanphan_cha2/aci-logo-v41.png" width="100" height="40"></a>&nbsp; &nbsp;&nbsp;</strong><a title="ศูนย์ดัชนีการอ้างอิงวารสารไทย:Thai Citation Index (TCI) centre" href="https://tci-thailand.org/" target="_blank" rel="noopener"><img title="tci" src="/public/site/images/tci_admin/tci.png" width="86" height="38" border="0"></a>&nbsp;&nbsp; &nbsp;<a title="Crossref" href="https://search.crossref.org/?q=ramathibodi&amp;container-title=Ramathibodi+Medical+Journal" target="_blank" rel="noopener"><img src="/public/site/images/nuanphan_cha2/images.png" width="77" height="50">&nbsp;</a>&nbsp; &nbsp;&nbsp;<a title="Google Scholar" href="https://scholar.google.co.th/citations?user=N7b2p9MAAAAJ&amp;hl=th&amp;authuser=1" target="_blank" rel="noopener"><img src="/public/site/images/nuanphan_cha2/scholar_logo_64dp_(1).png" width="155" height="26"></a></p> Faculty of Medicine Ramathibodi Hospital, Mahidol University, THAILAND en-US Ramathibodi Medical Journal 0125-3611 MicroRNA Expression of Primary and Metastatic Colorectal and Breast Carcinoma https://he02.tci-thaijo.org/index.php/ramajournal/article/view/257343 <p><strong>Background:</strong> Colorectal and breast carcinoma are frequently diagnosed cancers. At advanced stages, cancers metastasize to certain organs resulting in loss of function of these organs, and eventually death. Therefore, there is a specific need for the prognosis of these cancers. Currently, microRNAs (miRNAs), have emerged as a new target of cancer-specific biomarker.</p> <p><strong>Objective:</strong> To examine the expression of miRNA in primary and metastatic breast and colorectal cancers.</p> <p><strong>Methods:</strong> This study investigated the expression of 6 miRNAs (miR-10b, miR-21, miR-145, miR-155, miR-200c, and miR-373) in formalin fixed paraffin embed tissues from pairs of normal tissues with primary and metastatic tumor samples of breast and colorectal carcinoma cases in Ramathibodi Hospital, Thailand by real-time RT-PCR.</p> <p><strong>Results:</strong> Among 6 miRNAs, miR-145 decreased significantly in all samples of primary and metastatic colorectal and breast carcinoma. There was significantly decreased expression of miR-145 in metastatic colorectal carcinoma compared to their primary colorectal carcinoma (<em>P</em> &lt; .05). Whereas miR-10b, miR-155, and miR-200c showed a decreased expression; miR-21, and miR-373 showed an increased expression in the majority of cases. Unlike miR-145, other miRNAs showed no significant difference of expression (<em>P</em> &gt; .05).</p> <p><strong>Conclusions:</strong> This finding indicates that miR-145 may be the potential metastatic biomarker. Decrease of miR-145 could be applied to the prognosis and target for therapy of breast and colorectal carcinoma.</p> <p> </p> Thaniya Sricharunrat Artit Jinawath Pattana Sornmayura Sansanee Wongwaisayawan Budsaba Rerkamnuaychoke Copyright (c) 2022 Ramathibodi Medical Journal https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 1 13 10.33165/rmj.2022.45.3.257343 Usability and Acceptability of Love Thyroid Application in Patients With Hyperthyroidism Receiving Radioactive Iodine Treatment https://he02.tci-thaijo.org/index.php/ramajournal/article/view/258474 <p><strong>Background:</strong> Telemedicine and e-health services are crucial tools to help patients undergo complex treatments, such as radioactive iodine treatment (RIT), during the COVID-19 pandemic. However, the efficacy of such tools in real-life practice in Thailand is unknown.</p> <p><strong>Objective:</strong> To investigate the usability, acceptability, and user needs for the Love Thyroid application and book among patients with hyperthyroidism.</p> <p><strong>Methods:</strong> A descriptive study, 120 patients with hyperthyroidism who received RIT were given the chance to use both the Love Thyroid application and book. Then, all patients completed the electronic questionnaire that gathers information, including the usability, acceptability and user needs for both media.</p> <p><strong>Results:</strong> The Love Thyroid application was more helpful in following patients up after RIT and has a more attractive design than the book (<em>P</em> &lt; .05). The book was easier to search for information than the application (<em>P</em> &lt; .05). Furthermore, the application had higher acceptability than the book in terms of the confidence in data security, ease of handling, and satisfaction (<em>P</em> &lt; .05). The usefulness of the educational contents was comparable for both media. The application was more likely to be recommended by the patients to others than the book (<em>P</em> &lt; .05).</p> <p><strong>Conclusions:</strong> Mobile applications, such as the Love Thyroid application, had good usability and acceptability as informative tools for patients with hyperthyroidism underwent RIT.</p> <p> </p> Wipaporn Panwichai Alisara Wongsuttilert Nutthanon Leelathakul Sunisa Rimcharoen Pureepat Arttawejkul Copyright (c) 2022 Ramathibodi Medical Journal https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 14 22 10.33165/rmj.2022.45.3.258474 Knowledge and Attitude Towards Contraceptive Methods Among First-Year Nursing Students in Da Nang, Vietnam https://he02.tci-thaijo.org/index.php/ramajournal/article/view/257591 <p><strong>Background:</strong> Contraceptive methods have still been the sensitive topic in some Asia countries, including Vietnam. Improvement knowledge and attitude towards contraceptive methods would lead to better prevent unintended pregnancy and sexually transmitted disease as well as contributes to advance quality of life of young people even nursing students.</p> <p><strong>Objectives:</strong> To investigate knowledge and attitudes towards contraceptive methods, and identified the association between social demographic characteristics, attitudes, and knowledge towards contraception among first-year nursing students.</p> <p><strong>Methods:</strong> The cross-sectional study was conducted on 170 first-year nursing students, Da Nang University of Medical Technology and Pharmacy, Vietnam. Participants were selected according to the convenient sampling method. Data were collected from March to April 2021 through the questionnaire that consists of 2 parts including the sociodemographic characteristics and the set of questionnaires that assessed the participants’ knowledge and attitudes about contraceptive methods. Data were analyzed using descriptive statistics, chi-square test, Fisher exact test, and Spearman correlation test.</p> <p><strong>Results:</strong> Of 170 first-year nursing students, mean (SD) of contraceptive methods knowledge was 45.2 (8.5) (range, 25 - 62), illustrating a medium level. Attitude regarding contraceptive methods among first-year nursing students was negative (mean, 82.6; range, 49 - 95). The finding demonstrated a weakly positive correlation between attitude and knowledge (<em>P</em> &lt; .05). There was no significant association between social demographics and knowledge (<em>P</em> &gt; .05).</p> <p><strong>Conclusions:</strong> Knowledge and attitude towards contraceptive methods of first-year nursing students should be improved. Improvement knowledge and attitude contraception through education should be necessary for students.</p> <p> </p> Tran Anh Ngoc Hoang Thi Ngoc Sen Copyright (c) 2022 Ramathibodi Medical Journal https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 23 32 10.33165/rmj.2022.45.3.257591 The Surveys of Domestic Violence Among Families in Bangkok During the COVID-19 Pandemic https://he02.tci-thaijo.org/index.php/ramajournal/article/view/257258 <p><strong>Background:</strong> The global COVID-19 outbreak affects economic conditions at all levels, from global to family. Most people’s lifestyles have changed dramatically, including restrictions on movement and spending more time with their families. As a result, it may contribute in family problems, particularly domestic violence.</p> <p><strong>Objectives:</strong> To investigate the prevalence and impact of domestic violence on families in Bangkok during the COVID-19 pandemic.</p> <p><strong>Methods:</strong> A cross-sectional survey was conducted to investigate the prevalence of domestic violence and family factors related to changes in family violence in Bangkok, during 2020 to 2021. The sample consisted of 198 women over the age of 15, and data were collected by using women’s and family welfare questionnaire and analyzed by descriptive statistics, the McNemar statistical test, and the Wilcoxon signed rank test.</p> <p><strong>Results:</strong> The prevalence of domestic violence in Bangkok decreased from 42.9% from the first outbreak (January - May 2020) to 37.4% of the second outbreak (July 2020 - January 2021) but there was no statistically significant difference (<em>P</em> &gt; .05) between the 2 periods. However, in this study population, the most significant factors (<em>P</em> &lt; .05), such as family income per month, work impact, economic issue, and family stress were recognized.</p> <p><strong>Conclusions:</strong> This study highlighted that domestic violence among women during the COVID-19 pandemic has had remarkable negative economic, stress and social consequences for families.</p> <p> </p> Pittaya Sangkaew Rungtiwa Saowanee Araya Haupala Copyright (c) 2022 Ramathibodi Medical Journal https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 33 41 10.33165/rmj.2022.45.3.257258 Editor's Note https://he02.tci-thaijo.org/index.php/ramajournal/article/view/260201 <p style="text-align: justify;">Recently, there has been a survey study regarding publication ethics knowledge among 264 respondents from various countries.<sup>1</sup> The survey results reveal that only 36.7% were aware of International Committee of Medical Journal Editors (ICMJE) authorship criteria<sup>2</sup> while 44.3% reported including authors with no or limited significant contribution to an article.<sup>1</sup> Furthermore, only 42.4% of the respondents had been formerly trained in publication ethics (also known as research integrity or responsible conduct of research).</p> <p style="text-align: justify;">Although, the findings from the study may not represent publication ethics knowledge among Thai researchers. The results still point out international researchers’ lack of awareness and knowledge. In RMJ’s editorial experience, we have faced problems such as inappropriate authorship, plagiarism, or falsification. Dealing with these problems is often difficult. Some authors may quietly accept and try their luck elsewhere, while some authors angrily respond as an insult to their reputation. Journal editors should not just detect and reject the manuscript. As part of the scientific publication, RMJ editorial team believe that the prevention of future misconduct is also a responsibility of the journal editorial team. Beyond education, informing the authors’ institutions is essential to guarantee proper institutional oversight.</p> <p style="text-align: justify;">There is an organization called COPE (Committee on Publication Ethics)<sup>3</sup> which provides education and support for editors, publishers, and researchers regarding publication ethics. CPOE provides best practices and guidance for various problems and difficult situations regarding publication ethics.</p> <p style="text-align: justify;">&nbsp;</p> Chusak Okascharoen Copyright (c) 2022 https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 Autoimmune Hemolytic Anemia After Inactivated Virus COVID-19 Vaccination: A Report of 2 Cases https://he02.tci-thaijo.org/index.php/ramajournal/article/view/256357 <p>Very few cases of autoimmune hemolytic anemia (AIHA) have been reported after mRNA COVID-19 vaccinations. But herein we found 2 cases of AIHA after inactivated virus COVID-19 vaccination. Case 1: a 47-year-old Thai woman suffered from malaise, fatigue, and feverish feeling since the evening of the day of the first dose of inactivated virus COVID-19 vaccination (Sinopharm). She did not have any serious health problem prior this illness. Physical examination revealed marked pallor and body temperature was 38.1°C. Blood tests showed hemoglobin (Hb) 55 g/L, white blood cell (WBC) 12.2 × 10<sup>9</sup>/L, platelet 310 × 10<sup>9</sup>/L, nucleated red blood cell (RBC) 8/100 WBC, reticulocyte count 25.0%, direct antiglobulin test-4+ positive, indirect antiglobulin test-2+ positive. She was diagnosed with severe AIHA and well responsive to steroid therapy. Case 2: a 78-year-old Thai woman felt fatigue without fever 3 days after the second dose of inactivated virus COVID-19 vaccine (Sinopharm). Her underlying disease was anemia of the elderly with alpha thalassemia-1 heterozygosity. Her averaged Hb concentration before transfusion was 85 ± 3 g/L and she always needed transfusion every 2 months. Physical examination revealed only pallor. Blood test showed Hb 79 g/L, WBC 3.7 ×10<sup>9</sup>/L, platelet 104 × 10<sup>9</sup>/L, direct antiglobulin test-4+ positive, indirect antiglobulin test-4+ positive. She was diagnosed with AIHA and treated with cyclophosphamide and blood transfusion. Although the association between the inactivated virus COVID-19 vaccination and AIHA could not be simply concluded and the mix-and-match combination of different COVID-19 vaccines is promoted by Thai government, both of our patients chose to postpone the next dose of any kind of COVID-19 vaccine.</p> <p> </p> Somchai Insiripong Tanakorn Anantasetagoon Likhasit Sanglutong Copyright (c) 2022 Ramathibodi Medical Journal https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 42 47 10.33165/rmj.2022.45.3.256357 The Misdiagnosis of Beta-Thalassemia Heterozygosity Led by Hyperthyroidism https://he02.tci-thaijo.org/index.php/ramajournal/article/view/256975 <p>When patients have mild microcytic hypochromic anemia with slightly increased hemoglobin (Hb) A<sub>2</sub> fraction, the most likely diagnosis is beta-thalassemia heterozygosity. But herein we found a patient who had all these hematological parameters but did not have beta-thalassemia heterozygosity. He was a 14-year-old Thai who presented with fatigue and heat intolerance for 2 weeks. His physical examination revealed mild diffuse enlargement of thyroid gland. Blood tests showed Hb 120 g/L, mean corpuscular volume (MCV) 72.1 fL, mean corpuscular hemoglobin (MCH) 23.3 pg/cell, free triiodothyronine (FT<sub>3</sub>) &gt; 20 pg/mL, free thyroxine (FT<sub>4</sub>) &gt; 5.0 ng/dL, thyrotropin &lt; 2.5 mIU/L, serum ferritin 51.3 µg/L, Hb A<sub>2</sub> 3.8%. Besides primary hyperthyroidism, he was diagnosed with beta-thalassemia heterozygosity. After being treated with antithyroid drug for 6 months, his blood tests showed subclinical hyperthyroidism, Hb 146 g/L, MCV 83.3 fL, MCH 26.3 pg/cell, Hb A<sub>2</sub> 3.0%. Not only the thyroid hormones levels but also the Hb concentration, MCV, and the Hb A<sub>2</sub> percentage became normal. Due to this inconsistency, the DNA analysis for beta-thalassemia genes was performed and found negative for numerous common and rare beta-thalassemia genes meanwhile beta-globin gene sequencing appeared normal. It should be concluded that hyperthyroidism could induce slightly elevated Hb A<sub>2</sub> percentage and mild hypochromic microcytic anemia in a normal individual, leading to the misdiagnosis of beta-thalassemia heterozygosity. In other words, Hb analysis should not be performed during hyperthyroidism and it should be delayed till achievement of the euthyroid stage.</p> <p> </p> Likhasit Sanglutong Somchai Insiripong Wattana Insiripong Copyright (c) 2022 Ramathibodi Medical Journal https://creativecommons.org/licenses/by-nc-nd/4.0 2022-09-26 2022-09-26 45 3 48 53 10.33165/rmj.2022.45.3.256975