5
Siriraj Medical Bulletin
Evaluating the Eciency of
Dry Heat Pasteurization to
Kill Mycobacterium
Tuberculosis (In Vitro)
Nonthakorn Sakulwittayasuk*, Nattakan Thakaew*, Saijai Smithikarn* * * * *, Susan Assanasen* * *,
Manoon Leechawengwongs*, Phalin Kamolwat*****, Sanan Visuthisakchai****,
Angkana Chaiprasert**, Prapaporn Srilohasin**
*Drug-Resistant Tuberculosis Research Fund, Siriraj Foundation, under the Patronage of HRH Princess Galyani
Vadhana Krom Laung Narathiwas Rajnakarindth, **Research Division, Faculty of Medicine Siriraj Hospital, Mahidol
University, Bangkok, ***Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University,
Bangkok, ****Ofce of the Director, Siriraj hospital, Mahidol University, Bangkok, ***** Tuberculosis Division,
Department of Disease Control, Ministry of Public Health, Bangkok, Thailand.
Siriraj Medical Bulletin 2021;14(2): 5-11
Abstract
Objective: To evaluate the efciency of pasteurization for eliminating Mycobacterium
tuberculosis (in vitro). This study would provide informative data for taking care of tuberculosis
patients continuously and effectively during SARS-CoV-2 pandemic which N95 respirators
are shortage and required reusing.
Methods: The efciency of pasteurization was assessed against
M. tuberculosis
H37Rv. Pasteurization was performed at 72
o
C for 30 minutes with bacterial suspension at
different concentrations including 1-3x10
7
, 1-3x10
6
and 1-3x10
5
cells/ml. The growth of H37Rv
in pasteurized suspensions were monitored by using solid media Löwenstein– Jensen and
Ogawa media, and liquid medium (Mycobacterium Growth Indicator Tube; MGIT) for 8 weeks.
The assessment was duplicated for each concentration.
Result: Pasteurization at 72
o
C for 30 minutes signicantly eradicated
M. tuberculosis
H37Rv compared to control group (p-value < 0.01).
Conclusion: The study revealed that pasteurization completely destroy
M. tuberculosis
H37Rv (100%). In the circumstance of N95 constraint which reusing is required, pasteurization
is able to apply for eliminating M. tuberculosis. Our study provides informative data of the
decontamination of
M. tuberculosis
from N95 mask.
Keywords: pasteurization,
M. tuberculosis,
tuberculosis, N95 mask
Correspondence to: Prapaporn Srilohasin E-mail: prapaporn.srl@mahidol.ac.th
Received: 23 November 2020 Revised: 17 February 2021 Accepted: 5 March 2021
http://dx.doi.org/10.33192/Simedbull.2021.11
Siriraj Medical Bulletin
6 ี่� 14 ฉั่� 2 เม.ย - มิ.ย 2564
Original Article

Mycobacterium Tuberculosis
 (In vitro) 

 *,  *,  **,  ***,
 *,  **,  
****,  *,*****,
 *,*****
*     
   , **
  , *** 
 , ****  
, *****   

Mycobacterium tuberculosis



 M. tuberculosis
7

6





M. tuberculosis

M. tuberculosis

M. tuberculosis

7
Siriraj Medical Bulletin



(Mycobacterium tuberculosis) 















3


Mycobacterium tuberculosis
(M. tuberculosis)M. tuberculosis complex
















3






6








7

M. tuberculosis






 M. tuberculosis






M. tuberculosis

















7

8 ี่� 14 ฉั่� 2 เม.ย - มิ.ย 2564
Original Article

7

6







M. tuberculosis 
7

6









7

6






7

6





7

6










M. tuberculosis













 
Original Article
9
Siriraj Medical BulletinSiriraj Medical Bulletin

 

 
10 ี่� 14 ฉั่� 2 เม.ย - มิ.ย 2564
Original Article

 
 

      

 2 2 2

 2 2 2

5
 2 2 2




 
11
Siriraj Medical Bulletin












6






7












7



Pseudomonas
aeruginosa, Acinetobacter baumannii



































































