เวชั นึ กิ ิ าช
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SIRIRAJ MEDICAL BULLETIN
ISSN 2697-4436
https://he02.tci-thaijo.org/index.php/
simedbull/issue/current
Hyaluronan Binding Assay, Annexin-V/
Propidium Iodide Staining Acrosome Reaction
2019
:
Oxaliplatin
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Hyaluronan Binding Assay, Annexin-V/
Propidium Iodide Staining Acrosome Reaction
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Siriraj Medical Bulletin
Correlations Among Hyaluronan
Binding Assay, Annexin-V/Propidium
Iodide Staining and Acrosome
Reaction for Sperm Quality Assessment
*Department of Obstetrics and Gynecology, Faculty of Medicine, Naresuan University, Phitsanulok, **Infertility
Unit, Faculty of Medicine, Naresuan University, Phitsanulok, ***Basic Medical Science Research Unit, Faculty of
Medicine, Naresuan University, Phitsanulok, Thailand.
Siriraj Medical Bulletin 2022;15(2): 70-77
Surachai Dejarkom*, Suwanthana Yamthanod**, Warinda Phoonthaweerat**,
Kwansuda Supalap***, Patcharada Amatyakul*
Abstract
Objective: To evaluate correlation among Hyaluronan Binding Assay (HBA), Annexin-V binding and
Acrosome Reaction (AR) with semen analysis by World Health Organization (WHO) criteria.
Material & Methods: Twenty-seven infertile men underwent semen analysis at the infertility center,
Naresuan university hospital using basic quantitative method by WHO criteria and qualitative methods by
HBA, Annexin-V binding and AR. The results were calculated for correlations among these methods.
Results: The mean age of the study group was 34.2 ± 5.4 years old. The semen analysis showed nor-
mal volume, sperm concentration, progressive motility, total motility and morphology. The result of HBA was
87.5%. According to the annexin-V binding assay, most of sperm (80.47%) was viable without apoptosis. The
results of Sperm Spontaneous Acrosome Reaction Rate (SARR) and acrosome reaction after ionophore chal-
lenge were within normal ranges. There was no correlation between HBA and sperm parameters evaluated
by WHO criteria. However, HBA bound significantly correlated with viable sperm and HBA bound negatively
correlated with late apoptotic sperm (r
s
0.466, p = 0.014 and -0.563, p = 0.002, respectively).
Conclusion: Hyaluronic acid binding assay was correlated with viable sperm and low late apoptosis,
but not correlated with sperm acrosome reaction.
Keywords: semen analysis; correlation; hyaluronan binding assay; annexin-v binding assay; acrosome
reaction
Correspondence to: Surachai Dejarkom
Email: surachaid@nu.ac.th
Received: 21 September 2021
Revised: 29 November 2021
Accepted: 8 December 2021
http://dx.doi.org/10.33192/smb.v15i2.253684
Siriraj Medical Bulletin
Hyaluronan
Binding Assay, Annexin-V/ Propidium Iodide
Staining Acrosome Reaction
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Stressors of Patients with Adjustment
Disorders Who Were Admitted To
Siriraj Hospital And Factors Associated
with Stressors Vulnerability And
Suicide Attempt
Department of Psychiatry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Siriraj Medical Bulletin 2022;15(2): 78-85
Tichakorn Thongkleeb, Sirirat Kooptiwoot, Natee Viravan
Abstract
Objective: To study the factors associated with stressors vulnerability and suicide attempt in Siriraj
Hospital inpatients who were diagnosed with adjustment disorders.
Material & Methods: The analytical cross-sectional study of Siriraj Hospital inpatients who was
diagnosed with adjustment disorders. We collected patients’ demographic data, medical data, stressors,
and suicide attempt history from electronic medical records. The data were analyzed by descriptive
statistics, Chi-square test, independent t-test, and binary logistic regression.
Results: Of 370 samples, 272 (73.5%) had health problems as stressors, 80 (21.6%) had problems
with primary support group as stressors, and 74 (20.0%) attempted suicide. The factors associated with vul-
nerability to health problems rather than problems with primary support group were older age (10 years) [OR
1.8 (95%CI 1.4-2.3)] and moderate to severe physical illness comorbidities [OR 6.5 (95%CI 2.3-18.3)]. The
factors associated with vulnerability to problems with primary support group rather than health problems was
married [OR 3.5 (95%CI 1.7-7.4)]. The factors associated with higher risk of suicide attempt were younger age
(10 years) [OR 1.5 (95%CI 1.1-2.0)] and having problems with primary support group as a stressor [OR 25.8
(95%CI 11.8-56.4)].
Conclusion: Health problems and problems with primary support group were the main stressors
among inpatients with adjustment disorders. Older age or more severe physical illness comorbidities were
associated with more vulnerability to stress from health problems. Younger age or married status were asso-
ciated with more vulnerability to stress from problems with primary support group and higher risk for suicide.
Adjustment disorders screening for the high risk groups, physical health promotion, and family strengthening
might prevent adjustment disorders and suicide attempts.
Keywords: adjustment disorders; stressor; suicide
Correspondence to: Natee Viravan
Email: natee.vir@mahidol.ac.th
Received: 28 August 2021
Revised: 29 November 2021
Accepted: 14 December 2021
http://dx.doi.org/10.33192/smb.v15i2.253156
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The Development Of Hand Cream
Products from Stevia Rebaudiana
Bertoni Extract and Satisfaction
Assessment AmongVillage Health
Volunteers at Klang Yai Health
Promoting Hospital, Ban Phue District,
Udon Thani Province
*Kanchanabhishek Institute of Medical and Public Health Technology, Nonthaburi, **Klang Yai Health Pro-
moting Hospital, Udon Thani, Thailand
Siriraj Medical Bulletin 2022;15(2): 86-92
Wilailak Suksai*, Sathaporn Satsue*, Chatdanai Uparawanna*, Tawinta Tedthai*, Natchalida
Naruephatthanakit*, Kanyarat Phromphonchon**
Abstract
Objective: To develop of hand cream products from
Stevia Rebaudiana
Bertoni Extract and satisfac-
tion assessment among village health volunteers at Klang Yai health promoting hospital, Ban Phue district,
Udon Thani province.
Material & Methods: The research and development model was introduced by evaluating the qualities
and problems of on-market hand cream products from a group discussion. Then, develop the product with
physical and chemical properties evaluation, stability test and satisfaction assessment.
Results: The sample group which previously had problems with dry, dehydrated hand skin from occu-
pation related routines such as gardening and farming, looks for hand creams made from natural extracts that
can be easily bought. From the product development, hand cream with smooth texture, light green color with
scents of stevia clearly demonstrates as beneficial. The cream also holds appropriate viscosity and pH value
of 5-6, without microbial, fungal growth, nor Creaming-Cracking shown. The overall satisfaction scores at a
high level (x
= 4.4). In terms of its features, 3 best qualities scoring the highest in the satisfactory test are its
pleasant smell, absence of skin irritation and product design, x
= 4.6, 4.5, 4.6, in that order. Its convenience
for frequent use comes second best with the score of 4.4.
Conclusion: The results show that stevia extract can help increasing the effectiveness of hand skincare,
paving ways to the development of skincare with naturally extracted components. The results also provide alter-
native options to consumers while, potentially, increasing the market value of stevia for farmers.
Keywords:
Stevia Rebaudiana
Bertoni; hand cream; village health volunteers
Correspondence to: Wilailak Suksai
Email: wilailak.may555@gmail.com
Received: 28 January 2022
Revised: 14 March 2022
Accepted: 23 March 2022
http://dx.doi.org/10.33192/smb.v15i2.255905
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Siriraj Medical Bulletin
Guideline Practice of Thai Traditional
Medicine Treatment for Prevention
and Reducing Risk from COVID-19
Transmission
Center of Applied Thai Traditional Medicine (CATTM), Faculty of Medicine Siriraj Hospital, Mahidol University,
Bangkok, Thailand.
Siriraj Medical Bulletin 2022;15(2): 93-99
Jakkarin Wattanakittikul, Kodchakorn Somchat, Tiyapat Meechai, Viewwadee Boonleang, Darunee
Rattanawongsamathakul, Manmas Vannabhum, Supakij Suwannatrai, Pravit Akarasereenont
Abstract
The coronavirus disease (COVID-19) has spread in Thailand, causing social, economic, and healthcare
disruption at a large scale. The viral spread of coronavirus 2019 has influenced the government to reinforce an
Emergency Decree on Public Administration in Emergency Situations, social distancing, restrictions on business
sector and health care institutions. This affects those who need medical and surgical care. Ayurved Clinic Applied
Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University is aware of the importance of
reaching out to the patients during the pandemic. Therefore, a guideline of Thai traditional medicine treatment,
designed for medical providers and patients is crucial for prevention and screening COVID-19 transmission.
The guideline is composed of dividing workspace, initial screening for risk assessment, personnel protection,
telemedicine, and procedure safety. It was later shown that both personnel staff and patients provided a high satis-
faction score with the service. Additionally, during the service opening, there were no reports of COVID-19 cases
in Ayurved Clinic Applied Thai Traditional Medicine. This guideline is recommended for preventing and reducing
the risk of COVID-19 spread, and for setting a proper practice for healthcare personnel. It can also be adapted to
best suit other situations or venues if needed.
Keywords: Thai traditional medicine; massage; herbal medicine; telemedicine; infection
Correspondence to: Kodchakorn Somchat
Email: kodchakorn.som@mahidol.edu
Received: 27 April 2021
Revised: 21 June 2021
Accepted: 22 June 2021
http://dx.doi.org/10.33192/smb.v15i2.249949
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Nursing Role on Videourodynamic
Study (VUDS)
*Division of Perioperative Nursing, Department of Nursing, Siriraj Hospital, **Bhumibol Adulyadej Hospital,
Bangkok, ***Yala Hospital, Yala, Thailand.
Siriraj Medical Bulletin 2022;15(2): 100-106
Wimol Saekoui*, Supawit Luangphattarawong**, Sukree Semmard***
Abstract
The kidneys, ureters, urinary bladder and the urethra are the components of the urinary system whose
functions including waste and toxins excretion, blood pressure, volume and pH control, as well as electrolyte
and metabolite control. If abnormalities in urinary excretion control develop, The chances are that such de-
velopment could be the major cause of mortality. Urodynamic testing using Videourodynamic Study (VUDS)
is a standard method for assessing how well the lower urinary tract functions, by examining the bladder with
fluoroscopy. The test reveals the anatomy of the bladder, urethra and the urinary system overall. This allows
doctors to identify, if any, the cause of the problem and physical abnormalities, which then provide them with
an effective treatment and a follow up plan, accordingly. This article’s aim is to provide nurses, patients’ clos-
est assistants, with understanding of the elements and process of urodynamic testing using Videourodynamic
Study (VUDS). As a result, not only can they provide comprehensive care to patients physically and mentally
from the pre-examination phase to the post-examination phase, but also advice their family on the matter.
This leads to a well-cooperated intra examination, effective treatment, and ultimately, the prevention of com-
plications from the examination.
Keywords: nursing role; Videourodynamic Study (VUDS)
Correspondence to: Wimol Saekoui
Email: wimolsae@hotmail.com
Received: 19 October 2021
Revised: 14 December 2021
Accepted: 28 December 2021
http://dx.doi.org/10.33192/smb.v15i1.254358
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Nursing Care for Women with
Acute Vaginal Candidiasis
*Division of Obstetrics and Gynecology Nursing, Department of Nursing, Siriraj Hospital, ** Department
of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, ***Postpartum Ward
Department Of Nursing, Taksin Hospital, Medical Service Department, Bangkok, Thailand.
Siriraj Medical Bulletin 2022;15(2): 107-113
Porntip Rachapromma*, Chenchit Chayachinda**, Chanakan Kerdklinhom*, Waraporn Iamvijarn*,
Thitarat Thanasakthitikul***
Abstract
Vaginal Candidiasis (VC) is one of the most common causes that bring women to gynecologic clinics.
It is not a sexually transmitted infection but appears to be a consequence of vaginal dysbiosis. As
Candida
albicans
is the main etiologic organism, imidazole anti-fungal drug has widely been recommended as the
treatment of choice. Due to its nature of frequent recurrence, lifestyle modification and reduction of risk
factors also play an important role. Nurses are key persons in the counseling process, including knowledge
and risk factors assessment, health literacy promotion and lifestyle modification. Health literacy is delivered
through documents via QR codes and simulation of intravaginal drug administration via in-house model.
This article aims to review risk factors and nursing care in vaginal candidiasis.
Keywords: nursing care; risk factors; vaginal candidiasis
Correspondence to: Porntip Rachapromma
Email: porntip.rac@gmail.com
Received: 3 November 2021
Revised: 15 February 2022
Accepted: 17 February 2022
http://dx.doi.org/10.33192/smb.v15i2.254222
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Candida albicans
Candida albicans (C. al-
bicans) C. glabrata, C. tropicalis, C. nivariensis
Candida spp.
C. albicans
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Counselor: A Role of Nurse in
Psychological Therapy for
Hematological Cancer Patients
Undergoing Chemotherapy
*Division of Medical Nursing, Department of Nursing, Siriraj Hospital, Bangkok,**School of Nursing, Mae Fah
Luang University, Chiang Rai, ***Pediatric Bone Marrow Transplant, Pediatric Nursing Division, Nursing
Department, King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
Siriraj Medical Bulletin 2022;15(2): 114-122
Jarinthip Pumsri*, Pattarika Panya**, Julalak Thiplourlert***
Abstract
The need for psychological therapy is particularly crucial for hematologic cancer patients undergoing
chemotherapy. While patients physically suffer from the disease itself, side effects and complications from
chemotherapy drugs, they also experience psychological difficulties such as hopelessness, helplessness, fear,
anxiety and so on. All those can amplify and worsen their health and recovery process. Counseling is an effec-
tive tool for battling such hardships as it encourages patients to be aware of their problems, recognize their
needs, and utilize available resources along the way. Therefore, patients would be able to solve or handle the
problems well. Once effectively overcame difficulties, patients can gain, ultimately, both physical and psycho-
logical health. The counselor is an independent role and is required as one of nursing competencies identified
by Thailand Nursing and Midwifery Council. As a health counselor for hematologic cancer patients, nurses
apply knowledge regarding hematologic cancer, as well as counseling process and techniques. Utilizing a com-
bination of both roles: a nurse and a counselor, and 2 fields of knowledge: hematologic cancer and counseling,
is challenging for hematologic cancer patient care. Hence, this article aims to 1. Discuss the role of nurses as
counselor in psychological therapy for hematologic cancer patients during receiving chemotherapy. 2. Provide
knowledge about the process of counseling. This could provide and ensure proper standards of care as coun-
selor for the patients.
Keywords: nurses’ role; counselors; psychological care; hematological cancer patients.
Correspondence to: Jarinthip Pumsri
Email: noonnaka1@gmail.com
Received: 6 December 2021
Revised: 7 March 2022
Accepted: 9 March 2022
http://dx.doi.org/10.33192/smb.v15i2.255169
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Role of Nurses in The Prevention
and Management of Laryngospasm
in Cancer Patients Treated with
Oxaliplatin
*Division of Medical Nursing, Department of Nursing, Siriraj Hospital, Bangkok, ** Pediatric Bone Marrow
Transplant, Pediatric Nursing Division, Nursing Department, King Chulalongkorn Memorial Hospital,
Bangkok, ***Inpatient Department, Medicine, Samitivej Sukhumvit Hospital, Bangkok, Thailand.
Siriraj Medical Bulletin 2022;15(2): 123-130
Jarinthip Pumsri*, Julalak Thiplourlert**, Chutikarn Poolpoem***
Abstract
Laryngospasm is common in patients receiving Oxaliplatin. Patients with the condition commonly
show dyspnea or wheezing. If not assessed and managed quickly and accurately, may cause disabilities or be-
come life-threatening. Nurses caring for patients receiving Oxaliplatin must have knowledge and understand-
ing of the occurrence of laryngospasms in cancer patients, as well as the factors that cause such conditions to
worsen. In addition, possessing observational skills for abnormal symptoms. This is to prevent, lower the risk,
monitor and reduce the severity of laryngospasms. The authors reviewed the literature on laryngospasms in
Oxaliplatin-treated patients. As a result, it was found that the nursing guidelines consisted of two main parts.
First, advise as a preparation before the initiation of medication, which includes abstaining from drinking cold
water and ice to reduce the triggers that cause laryngospasms. Second, advise the patient on the leading
symptoms of laryngospasm, and how to act when occurs. By utilizing both parts with symptom monitoring
and medication management on patients with laryngospasm, nurses could respond by the discontinuation of
the drug, oxygen level adjustment or antihistamines or hydrocortisone use. It became apparent that patient’s
condition has improved and the severity of laryngospasms has been reduced. The practice of nursing care
will ensure the patient’s safety. It also effectively improves the quality of nursing care for cancer patients who
receive Oxaliplatin.
Keywords: nurses’ role; prevention; management; Oxaliplatin; laryngospasm
Correspondence to: Jarinthip Pumsri
Email: noonnaka1@gmail.com
Received: 6 December 2021
Revised: 18 February 2022
Accepted: 21 February 2022
http://dx.doi.org/10.33192/smb.v15i2.255168
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