Tissue Factor Expression of Endothelial Cell in Response to Atherosclerotic Risk Factors

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Weerasak Wongtiraporn
Pusadee Luenee
Nisarat Opartkiattikul
Wanida Wongtiraporn
Wanida Wongtiraporn
Somsak Laiwejpithaya
Suthi Sangkarat
Sujera Laiwejpithaya


Objective: To study the effect of serum from patients with atherosclerotic risk factors on the synthesis of endothelial tissue factor.
Methods: Serum from 30 diabetic patients, 30 hyperlipemic patients, 30 smokers and 30 normal serum were incubated with cultured endothelial cells from a human umbilical vein. The tissue factor of endothelial cells was measured using the assay that was developed in house after 24 hours incubation time.
Results: Smokersั serum can significantly cause the increase in endothelial tissue factor. The mean level of tissue factor induced by smokersั serum is 1.12 microunits/cell whereas the mean level of tissue factor induced by diabetic serum, hyperlipemic serum and normal serum is 0.4, 0.48 and 0.2 microunit/cell, respectively.
Conclusion: Smoking may increase the risk of thrombosis by increasing the tissue factor production of endothelial cells.


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Wongtiraporn, W. ., Luenee, P. ., Opartkiattikul, N. ., Wongtiraporn, W. ., Wongtiraporn, W. ., Laiwejpithaya, S. ., Sangkarat, S. ., & Laiwejpithaya, S. . (2020). Tissue Factor Expression of Endothelial Cell in Response to Atherosclerotic Risk Factors. Siriraj Medical Journal, 63(1), 1–3. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/240817
Original Article


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