@article{Suttinont_Nakphong_2009, title={Optimal Level of Sections for Definite Diagnosis in Transbronchial Biopsy and Pleural Biopsy Specimens}, volume={61}, url={https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246592}, abstractNote={<p>Objective: To determine optimal level of serial section in transbronchial and pleural biopsy that yield maximal definite<br>diagnosis.<br>Methods: A cross sectional study of 118 transbronchial biopsy and pleural biopsy specimens submitted with serial<br>sectioning in 3 levels were performed. Specimens of 1 mm. in diameter were serially cut and slides at levels I, II, III (120,<br>240 and 360 μm.) from initial exposure of tissue in paraffin blocks were studied, and specimens of 2-3 mm. in diameter<br>were cut at levels I, II, III (0, 120 and 240 μm.) after tissues in paraffin blocks were trimmed to expose maximal diameter.<br>Comparisons of diagnoses of each level were done.<br>Results: The percentages of definite diagnoses were 90.2, 96.1 and 93.2 in sections of level I, II and III, respectively.<br>Chronic granulomatous lesions were found in section level II more than other levels, but there was no statistical<br>significance. (P value 0.131, Chi-Square test)<br>Conclusion: Transbronchial and pleural biopsy specimens should be cut deep to level II, one slide for hematoxylin-eosin<br>staining and 3 unstained slides for further investigation.</p>}, number={3}, journal={Siriraj Medical Journal}, author={Suttinont, Panthep and Nakphong, Usanee}, year={2009}, month={May}, pages={123–125} }