TY - JOUR AU - Laokirkkiat, Pitak AU - Jareemit, Nida AU - Thanaboonyawat, Isarin AU - Smith, Hollie AU - Sriiam, Sukanya PY - 2019/02/15 Y2 - 2024/03/29 TI - Correlation between the Hypoosmotic SwellingT and DNA Fragmentation Assessed by the TUNEL Assay in Asthenozoospermia JF - Siriraj Medical Journal JA - Siriraj Med J VL - 71 IS - 1 SE - Original Article DO - 10.33192/Smj.2019.02 UR - https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/172683 SP - 8-13 AB - <p><strong>Objective</strong>: To study the correlation between the hypoosmotic swelling test (HOST) and DNA fragmentation in<br>asthenozoospermia assessed by the terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphatebiotin<br>nick end‐labeling (TUNEL) assay.<br><strong>Methods</strong>: This cross-sectional study was conducted in 27 semen samples obtained from infertile men with<br>asthenozoospermia. Both HOST and TUNEL assay were performed for each sample. The sperm swelling pattern<br>and positive apoptosis staining of individual spermatozoa were evaluated. HOST and TUNEL scores, and the<br>proportion of positive staining in each grade were calculated in each sample.<br><strong>Results</strong>: The results showed a negative correlation between HOST and TUNEL scores (r = -0.428, P = 0.026). Sperm<br>swelling grade A had a higher incidence of positive apoptosis staining when compared with other grades (P &lt; 0.01).<br>There was no statistically significant difference in positive apoptotic staining between other grades; nevertheless,<br>sperm swelling grade D tended to have a lower incidence of positive apoptosis staining.<br><strong>Conclusion</strong>: Based on the results of this study, HOST may be used as an optional test to identify DNA-intact<br>spermatozoa whereby sperm with a grade D swelling pattern should be selected preferentially for intracytoplasmic<br>sperm injection (ICSI), whereas sperm with a grade A swelling pattern should be avoided for ICSI.</p> ER -