TY - JOUR AU - Tantanate, Chaicharoen AU - Teyateeti, Metinee AU - Tientadakul, Panutsaya PY - 2020/04/03 Y2 - 2024/03/28 TI - Influence of Plasma Interferences on Screening Coagulogram and Performance Evaluation of the Automated Coagulation Analyzer Sysmex® CS-2100i JF - Siriraj Medical Journal JA - Siriraj Med J VL - 63 IS - 5 SE - Original Article DO - UR - https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/240998 SP - 151-156 AB - <p><strong>Background:</strong> Plasma interference is a problem for coagulation analyzers using a photo-optical detection method. Sysmex®<br>CS-2100i is a fully-automated coagulation analyzer which has been developed to reduce this problem.<br><strong>Objective:</strong> To evaluate the influence of interferences on prothrombin time (PT), activated partial thromboplastin time (APTT),<br>and fibrinogen analyzed by Sysmex® CS-2100i. The performance of this analyzer was also assessed in this study.<br><strong>Methods:</strong> Pooled plasma samples spiked with interfering substances including hemoglobin, bilirubin and lipid were used in<br>the interference study. Real patients’ samples with these interferences were also tested. Control materials and pooled samples<br>were used for precision, comparison, and carryover studies.<br><strong>Results:</strong> The PT, APTT, and fibrinogen could be analyzed in both plasma samples with interference added and real abnormal<br>patients’ plasma samples. The deviation of PT, APTT, and fibrinogen levels was not obvious, except for an upward trend<br>of fibrinogen level with increased hemoglobin. The highest % CV of PT, APTT, and fibrinogen were 1.24, 3.18, and 4.31,<br>respectively for within-run and 2.32, 2.11, and 6.06, respectively for between-run precision analysis. The correlation coefficients<br>of PT, APTT, and fibrinogen between Sysmex® CS-2100i and Sysmex® CA-1500 were 0.99, 0.99, and 0.98 with intra-class<br>correlation coefficients of 0.99, 0.99, and 0.98 respectively. No significant carryover of specimen was observed.<br><strong>Conclusion:</strong> Sysmex® CS-2100i could analyze both samples with artificial interfering substance added and real abnormal patients’<br>samples. This machine also had an acceptable performance by our evaluation.</p> ER -