Comparison between egg white and milk served as preservative media for periodontal ligament cell viability and proliferation
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Abstract
The aim of this in vitro study was to compare periodontal tissue cell viability and proliferative activity after preservation of the avulsed tooth in milk and egg. Extracted human permanent molar teeth were divided into 3 groups of preservative media including milk, egg white and Dulbecco’s Modified Eagle’s Medium (DMEM) as control. The teeth were stored for 3 hours in the media and the periodontal ligament (PDL) tissues were then isolated for cell culture. The results showed no significant difference in periodontal ligament cell (PDLC) viability between milk and egg white groups. In term of proliferative activity, egg white group showed significantly lower percentage of proliferation compared with milk and DMEM. In conclusion, both milk and egg white could maintain PDLC viability. Milk storage resulted in higher cell proliferation than storing in egg white. However, egg white can be used as an alternative choice for preservative media for an avulsed tooth.
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References
Glendor U. Epidemiology of traumatic dental injuries - A 12 year review of the literature. Dent Traumatol. 2008;24:603-11.
World Health Organization. Application of the international classification of diseases to dentistry and stomatology: ICD-DA. 2nd ed. Geneva: World Health Organization; 1978.
Andreasen JO. Effect of extra-alveolar period and storage media upon periodontal and pulpal healing after replantation of mature permanent incisors in monkeys. Int J Oral Surg. 1981;10:43-53.
Fonseca RJ, Walker RV, Barber HD, Powers MP, Frost DE. Oral and maxillofacial trauma. 4th ed. St. Louis: Elsevier Saunders; 2013.
Trope M, Friedman S. Periodontal healing of replanted dog teeth stored in Viaspan, milk and Hank’s balanced salt solution. Endod Dent Traumatol. 1992;8:183-8.
Trope M. Clinical management of the avulsed tooth: present strategies and future directions. Dent Traumatol. 2002;18:1-11.
Sigalas E, Regan JD, Kramer PR, Witherspoon DE, Opperman LA. Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth. Dent Traumatol. 2004;20:21-8.
Oswald RJ, Harrington GW, Van Hassel HJ. A postreplantation evaluation of air-dried and saliva-stored avulsed teeth. J Endod. 1980;6:546-51.
Andreasen JO, Kristerson L. The effect of limited drying or removal of the periodontal ligament. Periodontal healing after replantation of mature permanent incisors in monkeys. Acta Odontol Scand. 1981;39:1-13.
Andersson L, Bodin I. Avulsed human teeth replanted within 15 minutes-A long-term clinical follow-up study. Endod Dent Traumatol. 1990;6:37-42.
Andreasen JO, Andreasen FM. Textbook and colour atlas of traumatic injuries to the teeth. St Louis: Mosby; 1994.
Chamorro MM, Regan JD, Opperman LA, Kramer PR. Effect of storage media on human periodontal ligament cell apoptosis. Dent Traumatol. 2008;24:11-6.
Poi WR, Sonoda CK, Martins CM, Melo ME, Pellizzer EP, de Mendonca MR, et al. Storage media for avulsed teeth: a literature review. Braz Dent J. 2013;24:437-45.
Thakur B, Pawar AM. Storage media used for avulsed teeth- The first aid. Eur J Pharm Med Res. 2017;4:433-7.
American Association of Endodontists. ENDODONTICS: Colleagues for Excellence. The Treatment of Traumatic Dental Injuries. [Internet]. 2014. [cited 2019 Jun 14]. Available from: https://www.aae.org/specialty/wp content/uploads/sites/2/2017/06/ecfe_summer2014-final.pdf.
McIntyre JD, Lee JY, Trope M, Vann WF Jr. Permanent tooth replantation following avulsion: using a decision tree to achieve the best outcome. Pediatr Dent. 2009;31:137-44.
Blomlof L, Lindskog S, Andersson L, Hedstrom KG, Hammarstrom L. Storage of experimentally avulsed teeth in milk prior to replantation. J Dent Res. 1983;62:912-6.
Khademi AA, Saei S, Mohajeri MR, Mirkheshti N, Ghassami F, Torabinia N, et al. A new storage medium for an avulsed tooth. J Contemp Dent Pract. 2008;9:25-32.
Wu G, Bryant MM, Voitle RA, Roland DA Sr. Effect of dietary energy on performance and egg composition of Bovans White and Dekalb White hens during phase I. Poult Sci. 2005;84:1610-5.
Udoye CI, Jafarzadeh H, Abbott PV. Transport media for avulsed teeth: a review. Aust Endod J. 2012;38:129-36.
Sharma M, Sharma S, Reddy YG, Mittal R, Agarwal V, Singh C, et al. Evaluation of periodontal ligament cell viability in three different storage media: an in vitro study. J Dent (Tehran). 2015;12:524-31.
Khademi AA, Atbaee A, Razavi SM, Shabanian M. Periodontal healing of replanted dog teeth stored in milk and egg albumen. Dent Traumatol. 2008;24:510-4.
Hasan MR, Takebe H, Shalehin N, Obara N, Saito T, Irie K. Effects of tooth storage media on periodontal ligament preservation. Dent Traumatol. 2017;33:383-92.
Thadavirul N, Pavasant P, Supaphol P. Development of polycaprolactone porous scaffolds by combining solvent casting, particulate leaching, and polymer leaching techniques for bone tissue engineering. J Biomed Mater Res A. 2014;102:3379-92.
Saxena P, Pant VA, Wadhwani KK, Kashyap MP, Gupta SK, Pant AB. Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study. Dent Traumatol. 2011;27: 102-8.
Badakhsh S, Eskandarian T, Esmaeilpour T. The use of aloe vera extract as a novel storage media for the avulsed tooth. Iran J Med Sci. 2014;39:327-32.
Courts FJ, Mueller WA, Tabeling HJ. Milk as an interim storage medium for avulsed teeth. Pediatr Dent. 1983;5:183- 6.
Is Khinda V, Kaur G, S Brar G, Kallar S, Khurana H. Clinical and practical implications of storage media used for tooth avulsion. Int J Clin Pediatr Dent. 2017;10:158-65.
Bag I, Yildirim S. Effect of avulsion storage media on periodontal ligament fibroblast differentiation. Dent Traumatol. 2017;33:458-64.
Salujah KS, Anegundi RT. Assessment of viability of human periodontal ligament cells in different fat content of milk at different time intervals. Int J Contemp Med Res. 2016;3:1376-9.
Anegundi RT, Daruwalla SF. Assessment of viability of periodontal ligament cells in different storage media. Int J Oral Health Med Res. 2016;3:21-7.
Goswami M, Chaitra T, Chaudhary S, Manuja N, Sinha A. Strategies for periodontal ligament cell viability: an overview. J Conserv Dent. 2011;14:215-20.
Blomlof L, Otteskog P, Hammarstrom L. Effect of storage in media with different ion strengths and osmolalities on human periodontal ligament cells. Scand J Dent Res. 1981;89:180-7.
Doyle DL, Dumsha TC, Sydiskis RJ. Effect of soaking in Hank’s balanced salt solution or milk on PDL cell viability of dry stored human teeth. Endod Dent Traumatol. 1998; 14:221-4.
Pohl Y, Filippi A, Kirschner H. Results after replantation of avulsed permanent teeth. II. Periodontal healing and the role of physiologic storage and antiresorptive-regenerative therapy. Dent Traumatol. 2005;21:93-101.
Andersson L, Andreasen JO, Day P, Heithersay G, Trope M, DiAngelis AJ, et al. Guidelines for the management of traumatic dental injuries: 2. avulsion of permanent teeth. Pediatr Dent. 2017;39:412-9