Anti-allergic activity of Trikatuk Tripha and Trisarn remedies

Abstract

Introduction: Trikatuk remedy (TK) has long been used in Thai traditional medicine for adaptogen or immunomodulatory agent and for treating diseases in the rainy season. Triphala remedy (TP) is used to adjust patients’ elements in summer. Trisarn remedy (TS) is a Thai traditional medicine used in winter. Ya-Tri-Pi-kad is a Thai traditional medicine for adaptogens. It is composed of 9 plants. It is on National herbal drug list of Thailand. The objective of this research was to investigate the anti-allergic activity of TK, TP and TS remedies.
Method: Three remedies were extracted by 95% ethanolic and boiling in water and plants ingredients were extracted by 95% ethanol. All extracts were calculated the percentage of yield and tested for anti-allergic activity by the inhibition of β-hexosaminidase release from RBL-2H3 cell lines.
Result: The results showed that the ethanolic extract of TK displayed the highest anti-allergic activity with IC50 = 38.02 µg/ml followed by the ethanolic extract of Piper nigrum and the ethanolic extractof Piper retrofractum (IC50 = 44.97 and 50.91 µg/ml, respectively) but were not significantly different from standard chlorpheniramine (CPM) with 26.12 ± 1.89 µg/ml (p-value < 0.05).
Discussion and Conclusion: The ethanolic extract of TK and its plants ingredients showed the most potent of anti-allergic activity. These results can support using Trikatuk remedy in Thai traditional medicine for adaptogen and allergy-related diseases.