Antioxidant, anti-allergic and anti-inflammatory activities and total phenolic compounds of Tregaysornmas formula

Abstract

Introduction: Tregaysornmas formula, including Jatropha multifida L., Nelumbo nucifera Gaertn. and Aegle marmelos (L.) Corrêa, was discovered in Thai Pharmacy scripture. In Thai traditional medicine, it has been used to treat fatigue and maintain body balance of patients. The objective of this study was to investigate the antioxidant, anti-allergic and anti-inflammatory activities of Tregaysornmas formula and determine their total phenolic content.
Methods: Two extraction methods (maceration and decoction) were used to extract Tregaysornmas formula. DPPH assays were used to evaluate antioxidant activity. Inhibitory effects on the release of β-hexosaminidase and nitric oxide inhibitory assay were used to evaluate anti-allergic and anti-inflammatory activities. Folin-Ciocalteu’s reagent was used to determine total phenolic
content.
Results: The 95% ethanol and water extracts displayed the high antioxidant activity on DPPH assay (EC50 = 3.86 ± 0.11 and 16.00 ± 3.60 μg/ml, respectively). Furthermore, both extracts showed
the total phenolic content with 300.24 ± 2.23 and 184.92 ± 4.33 mg GAE/g, respectively. For anti-allergic activity, the 95% ethanol extract exhibited the potent allergic activity by inhibitory effects on the release of β-hexosaminidase (IC50 = 93.51 ± 0.83 μg/ml), whereas, water extract had no anti-allergic activity. In addition, both were not show anti-inflammatory activity on nitric
oxide inhibitory assay.
Conclusion: Tregaysornmas formula showed antioxidant and ant-allergic activities and revealed high total phenolic compounds. These results support the use of Tregaysornmas formula in Thai traditional medicine.