Effect of Steaming Process on Chemical Content and Biological Activity of Curcuma zedoaria Extract
Introduction: The rhizomes of Curcuma zedoaria Roscoe have been used to treat infection and inflammation in the Thai traditional medicine. The traditional preparation of C.zedoaria is done performed by steaming the rhizome before drying and using. There has been no information about the effect of the steaming on the chemical components and the activity of C.zedoaria. In this report, therefore, we compare the chemical content, anti-inflammatory and antimicrobial activities of steamed, and non-steamed rhizomes of C.zedoaria.
Methods: The steamed and non-steamed rhizomes of C.zedoaria were extracted by maceration with 50% and 95% ethanol to obtain the ethanolic extracts. Then, the extracts were analyzed for curcuminoid content by HPLC technique, antimicrobial activity and anti-inflammatory activity by nitric oxide (NO) production inhibition assay.
Results: The highest curcuminoid content (152.23 ± 1.80 mg/g of dried extract) was found in the 95% ethanolic extract of the steamed rhizome. Both the ethanolic extracts of the steamed and non-steamed rhizomes exhibited anti-microbial activity against S.aureus, MRSA and C.albicans. The 95% ethanolic extract of the steamed rhizomes showed the highest inhibition effect on nitric oxide production with IC50 value of 11.22 ± 1.21 μg/mL.
Conclusion: From these results, it was concluded that the steaming process can increase the curcuminoid content and biological activities of C.zedoaria rhizomes. The best solvent for extraction of this plant part is 95% ethanol which give the highest percent yield of extract and strongest antimicrobial and anti-inflammatory activities.
2. Paramapojn S, Gritsanapan W. Free radical scavenging activity determination and quantitative analysis of curcuminoids in Curcuma zedoaria rhizome extracts by HPLC method. Current Science. 2009;97(7):1069-1073.
3. Dosoky NS, Setzer WN. Chemical composition and biological activities of essential oils of Curcuma species. Nutrients. 2018;10(9):1196.
4. Mun SH, Joung DK, Kim YS, et al. Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine. 2013;20(8-9):714-718.
5. Wilson B, Abraham G, Manju VS, et al. Antimicrobial activity of Curcuma zedoaria and Curcuma malabarica tubers. Journal of Ethnopharmacology. 2005;99(1):147-151.
6. Uechi S, Ishimine Y, Hongo F. Antibacterial activity of essential oil derived from Curcuma sp. (Zingiberaceae) against foodborne pathogenic bacteria and its heat stability. Science Bulletin of the College of Agriculture-University of the Ryukyus (Japan). 2000;47:129–136.
7. Makabe H, Maru N, Kuwabara A, Kamo T, Hirota M. Anti-inflammatory sesquiterpenes from Curcuma zedoaria. Natural product research. 2006;20(7):680-685.
8. Mau JL, Lai EY, Wang NP, Chen CC, Chang CH, Chyau CC. Composition and antioxidant activity of the essential oil from Curcuma zedoaria. Food Chemistry. 2003;82(4):583-
9. Roekruangrit N, Jaiarree N, Itharat A, et al. Comparative Study on Biological Activities of Steamed and Non-Steamed Ginger Extracts. Science & Technology Asia. 2019;24(4):
10. Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007;42(4):321-324.
11. Elshikh M, Ahmed S, Funston S, et al. Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants.
Biotechnology letters. 2016;38(6):1015-1019.
12. Makchuchit S, Rattarom R, Itharat A. The anti-allergic and anti-inflammatory effects of Benjakul extract (a Thai traditional medicine), its constituent plants and its some pure constituents using in vitro experiments. Biomedicine & Pharmacotherapy. 2017;89:1018-1026.
13. Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin.
Journal of agricultural and food chemistry. 2002;50(13):3668-3672.
14. Zhan PY, Zeng XH, Zhang HM, Li HH. High-efficient column chromatographic extraction of curcumin from Curcuma longa. Food chemistry. 2011;129(2):700-703.
15. Naczk M, Shahidi F. Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. Journal of pharmaceutical and biomedical analysis. 2006;41(5):1523-1542.
16. Mun SH, Joung DK, Kim YS, et al. Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine. 2013;20(8-9):714-718.
17. Gunes H, Gulen D, Mutlu R, Gumus A, Tas T, Topkaya AE. Antibacterial effects of curcumin: an in vitro minimum inhibitory concentration study. Toxicology and industrial
18. Khan N, Shreaz S, Bhatia R, et al. Anticandidal activity of curcumin and methyl cinnamaldehyde. Fitoterapia. 2012;83(3):434-440.
19. Luo J, Yang M. Demethoxycurcumin: a potential antimicrobial agent. Journal ofThermal Analysis and Calorimetry. 2013;115(3): 2331-2338.
20. Bugno A, Nicoletti MA, Almodóvar AA, Pereira TC, Auricchio MT. Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with
commercial mouthrinses. Brazilian Journal of Microbiology. 2007;38(3):440-445.
21. Lee TK, Trinh TA, Lee SR, et al. Bioactivitybased analysis and chemical characterization of anti-inflammatory compounds from Curcuma zedoaria rhizomes using LPS-stimulated RAW264. 7 cells. Bioorganic chemistry. 2019;82:26-32.
22. Ullah HA, Zaman S, Juhara F, et al. Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC
complementary and alternative medicine. 2014;14(1):346.
23. Kaushik ML, Jalalpure SS. Evaluation of antiinflammatory effect of ethanolic and aqueous extracts of Curcuma zedoaria Rosc root. Internation Journal of Drug Development &
24. Kim KI, Shin KS, Jun WJ, et al. Effects of polysaccharides from rhizomes of Curcuma zedoaria on macrophage functions. Bioscience, biotechnology, and biochemistry.
25. Zhang LJ, Wu CF, Meng XL, et al. Comparison of inhibitory potency of three different curcuminoid pigments on nitric oxide and tumor necrosis factor production of rat primary microglia induced by lipopolysaccharide. Neuroscience letters. 2008;447(1):48-53.
26. Zhang L, Wu C, Zhao S, et al. Demethoxycurcumin, a natural derivative of curcumin attenuates LPS-induced pro-inflammatory responses through down-regulation of intracellular ROS-related MAPK/NF-KB signaling pathways in N9 microglia induced by lipopolysaccharide. International immunopharmacology. 2010;10(3):331-338.
27. Priyanka R, Vasundhara M, Jayaram A, Nuthan D. A study on curcuminoid profile of Curcuma longa L. varieties as affected by processing method. Journal of Spices and
Aromatic Crops. 2016;25(1):26-33.
28. Keereekoch T, Srisuwan T, Taleh R, Hemtrakoonwong R, Chesa-ES, Subhadhirasakul S. Effects of Steam Sterilization Time and Temperature on Quality of Turmeric Powder.
Thaksin University Journal. 2014;17(1):57-67.
29. Tako M, Tamaki Y, Teruya T, Takeda Y. The principles of starch gelatinization and retrogradation. Food and Nutrition Sciences. 2014;5(3):280-291.