Increased Urinary Excretions of Oxidative Stress Biomarkers and Sialic Acid Associated with Severity of Bladder Tumors

Authors

  • Chavalit Honglertsakul Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Julin Opanuraks Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Wipawee Kittikowit Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Chanchai Boonla Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Rattiporn Wunsuwan Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Piyaratana Tosukhowong Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Abstract

Introduction: Reactive oxygen species-induced damage to lipids, proteins and DNA plays a major role in carcinogenesis. This study aimed to measure urinary excretions of 8-hydroxydeoxyguanosine (8-0HdG), malondialdehyde (MDA) and total sialic acid (TSA) in patients with bladder tumor and to determine whether their excretory levels were associated with severity of the tumors.

Materials and Methods: Morning urine samples were collected from 48 patients with bladder tumor and 30 healthy subjects. Concentration of creatinine, proteins, 8-OHdG, MDA and TSA were measured in the urine samples. The severity of transitional cell carcinoma of the bladder (TCC) was assessed by urine cytology (grading C1 to C5). The bladder tumor patients were classified into 2 groups, patients with negative urinary malignant cells (C1 and C2; n = 28) and those with positive urinary malignant cells (C3 to C5; n= 20).

Results: Levels of urinary proteins, 8-OHdG, MDA and TSA in patients with bladder tumor were significantly higher than in heal thy controls (p = 0.002, p = 0.009, p <0.001 and p <0.001, respectively). Urinary proteins, 8-OHdG, MDA and TSA levels in patients with C3 to C5 tumors were significantly higher than in those with C1 and C2 tumors (p = 0.043, p = 0.007, p = 0.024 and p = 0.018, respectively). In addition, urinary TSA levels in patients with C1 and C2 tumors were significantly higher than in healthy controls (p = 0.004).

Conclusions: The present study showed that urinary excretions of oxidative stress biomakers and TSA were significantly higher in bladder tumor patients and their levels correlated with the severity of the tumor. These noninvasive determinations may be clinically useful for diagnosing, prognosing and monitoring bladder cancer. Reduction of oxidative stress is recommended for primary prevention of bladder tumor.

References

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Published

2007-12-27

How to Cite

1.
Honglertsakul C, Opanuraks J, Kittikowit W, Boonla C, Wunsuwan R, Tosukhowong P. Increased Urinary Excretions of Oxidative Stress Biomarkers and Sialic Acid Associated with Severity of Bladder Tumors. Thai J Surg [Internet]. 2007 Dec. 27 [cited 2024 Dec. 23];28(4):133-7. Available from: https://he02.tci-thaijo.org/index.php/ThaiJSurg/article/view/241050

Issue

Vol. 28 No. 4 (2007): October - December

Section

Original Articles

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Articles must be contributed solely to The Thai Journal of Surgery and when published become the property of the Royal College of Surgeons of Thailand. The Royal College of Surgeons of Thailand reserves copyright on all published materials and such materials may not be reproduced in any form without the written permission.