Comparison of the Mini Parasep SF kit and Modified Kato-Katz Techniques for Screening The eggs of Opisthorchis viverrini in Fieldwork of Regional Health 9

Abstract

Opisthorchis viverrini (OV) infection is still endemic areas with the highest incidence of liver fluke infections
among in the north-eastern region of Thailand. Most at risk groups are asymptomatic and usually undiagnosed of stool
for screening the eggs of OV. Fieldwork using the Modified Kato-Katz (MKK) methods for prevalence and intensity of
infections. In 2016, the Office of Disease Prevention and Control 9th Nakhon Ratchasima, has added a new diagnostic tool
is Mini Parasep SF kit (MPSF kit). The prevalence rates of MKK 16.3%, MPSF kit 6.4%, which gives different results.
A cross-sectional study to comparison of two tools. With the data from registry of liver fluke 12 areas (settings include
Nakhon Ratchasima 3 areas, Chaiyaphum 2 areas, Buriram 4 areas and Surin 3 areas) total 2,148 cases analyzed by
statistical techniques. The results of this study showed that proportion agreement (Po) 83.9% (Ppos 29.9%, Pneg 90.9%),
with fair agreement the two tools calculate Cohon’s kappa (kappa = 0.23, 95%CI; 0.18-0.28), when compared with
MPSF kit as the MKK, the tool had a sensitivity 20.6%, specificity 96.6%, PPV 54.7% and NPV 85.9%. However,
the survey of prevalence and intensity of OV in fieldwork, the MKK method is still appropriate for the community. A new
diagnostic tool (MPSF kit) which include closed system, safety, if the small amount of the sample stool not detected,
although the level of agreement and sensitivity were low for both, this method is a useful screening technique were not
able to detect the eggs of OV in some cases. Particularly, assays for detect infective larval stage parasites and protozoa
cysts. Therefore, the selection the MKK used in fieldwork and long-term, should take into account the ease of use and
efficient management of limited resources for primary care unit.