Studies on diarrheagenic Escherichia coli isolated from rectal swab patients in Thammasat University Hospital
Keywords:
diarrheagenic Escherichia coli (DEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC),, enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enterohaemorrhagic E. coli (EHEC))Abstract
Background : Diarrheagenic Escherichia coli (DEC) causing diarrhea is an important problem both in developed countries and developing countries including Thailand. However, microbiology laboratories in the hospitals do not detect DEC in routine work.
Objective : This study was to detect DEC from rectal swabs patients in Thammasat Hospital.
Material and methods : Seventy samples of rectal swab were enriched in tryptic soy broth for preparing boiled templates. The PCR method for investigation of DEC was used to detect a) aggR and Eagg for enteroaggregative E. coli (EAEC), b) bfpA and eaeA for enteropathogenic E. coli (EPEC), c) elt and est for enterotoxigenic E. coli (ETEC), d) invE for enteroinvasive E. coli (EIEC), e) eaeA, stx1 and stx2 for enterohaemorrhagic E. coli (EHEC).
Result : From this result, none of EPEC, ETEC, EHEC and EIEC was detected while 5 isolates of EAEC (7.14%) were detected in 5 rectal swab samples. These 5 EAEC isolates were subcultured and tested antimicrobial susceptibility test. Of all 5 EAEC isolates, the resistances to ampicillin, co-trimoxazole and gentamicin were 100% (5/5), 60% (3/5) and 40% (2/5), respectively. Eighty percent of isolates were found under the age of 2 years.
Conclusion : These DEC may be associated with diarrheal children, therefore, Identification of these strains are helpful for treatment. Furthermore, epidemiologic surveillance for DEC would be useful for diarrheal control and prevention in children.
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