Effect of Lactobacillus rhamnosus SD11 as a probiotic in yogurt on oral microbial and caries outcomes in 12-15 years old children: A double blind randomized controlled trial
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Abstract
This study aims to evaluate effects of probiotic Lactobacillus rhamnosus SD11 on salivary mutans streptococci and lactobacilli count, and dental caries in children. A double-blind randomized control trial was conducted. A total of 102 children, aged 12-15 years, were recruited, and randomly assigned to either the probiotic group receiving yogurt containing L. rhamnosus SD11 or control group receiving standard yogurt containing L. bulgaricus. One hundred milliliters of yogurt were provided once daily for 3 months. The salivary samples were collected at baseline (T0), 3 months (T3), 6 months (T6) and 9 months (T9) for counting the numbers of mutans streptococci and lactobacilli. Dental caries was examined at baseline and at the end of study (T9). At T3, mutans streptococci in probiotic group was significantly lower (p<0.05) than the control group and a significant reduction (p<0.05) were found when compare with T0. Simultaneously, the numbers of lactobacilli in probiotic group were significantly increased (p<0.01) at T3 when compared with T0 and with control group. However, there were no significant differences between the groups at T6 and T9. The caries progression (transitional probability) was not significantly different between groups. No adverse event was found in both groups. In conclusion, the consumption of L. rhamnosus SD11 daily for 3 months is safe. The increasing of lactobacilli and reduction of cariogenic pathogen, mutans streptococci were found within 3 months of the study. However, the effect to prevent caries progression was not presented.
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References
Petersen PE, Ogawa H. Prevention of dental caries through the use of fluoride-the WHO approach. Community Dent Health 2016; 33(2): 66-8.
Bureau of Dental Health. The 8th national oral health survey 2017 of Thailand. Bangkok: Samcharoen-panit Co.; 2018. (in Thai)
Lemos JA, Palmer SR, Zeng L, Wen ZT, Kajfasz JK, Freires IA, et al. The biology of Streptococcus mutans. Microbiol Spectr 2019; 7(1): 1-26. doi:10.1128/microbiolspec. GPP3-0051-2018.
World Health Organization, Food and Agriculture Organization of the United Nations. Probiotics in food health and nutritional properties and guidelines for evaluation. Rome: World Health Organization, Food and Agriculture Organization of the United Nations; 2006.
Twetman S, Stecksén-Blicks C. Probiotics and oral health effects in children. Int J Paediatr Dent 2008; 18(1): 3-10. doi:10.1111/ j.1365-263X.2007.00885.x.
Pramanik S, Venkatraman S, Karthik P, Vaidyanathan VK. A systematic review on selection characterization and implementation of probiotics in human health. Food Sci Biotechnol 2023; 32(4): 423-40. doi:10.1007/ s10068-022-01210-z.
Homayouni Rad A, Pourjafar H, Mirzakhani E. A comprehensive review of the application of probiotics and postbiotics in oral health. Front Cell Infect Microbiol 2023; 13:1-19. doi:10.3389/fcimb.2023.1120995.
Sivamaruthi BS, Kesika P, Chaiyasut C. A review of the role of probiotic supplementation in dental caries. Probiotics Antimicrob Proteins 2020; 12(4): 1300–9. doi:10.1007/s12602-020-09652-9.
Bermudez-Brito M, Plaza-Díaz J, Muñoz-Quezada S, Gómez-Llorente C, Gil A. Probiotic mechanisms of action. Ann Nutr Metab 2012; 61(2): 160-74. doi:10.1159/000 342079.
Piwat S, Teanpaisan R, Thitasomakul S, Thearmontree A, Dahlén G. Lactobacillus species and genotypes associated with dental caries in Thai preschool children. Mol Oral Microbiol 2010; 25(2): 157-64. doi:10.11 11/j.2041-1014.2009.00556.x.
Teanpaisan R, Piwat S. Lactobacillus paracasei SD1, a novel probiotic, reduces mutans streptococci in human volunteers: A randomized placebo-controlled trial. Clin Oral Investig 2014; 18(3): 857-62. doi:10.100 7/s00784-013-1057-5.
Teanpaisan R, Piwat S, Tianviwat S, Sophatha B, Kampoo T. Effect of long-term consumption of Lactobacillus paracasei SD1 on reducing mutans streptococci and caries risk:a randomized placebo-controlled trial. Dent J 2015; 3(2): 43-54. doi:10.3390/dj 3020043.
Piwat S, Sophatha B, Teanpaisan R. An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity. Lett Appl Microbiol 2015; 61(1): 98-105. doi:10.11 11/lam.12434.
Teanpaisan R, Piwat S, Dahlén G. Inhibitory effect of oral Lactobacillus against oral pathogens. Lett Appl Microbiol 2011; 53(4): 452-9. doi:10.1111/j.1472-765X.2011.03132.x.
Rungsri P, Akkarachaneeyakorn N, Wongsuwanlert M, Piwat S, Nantarakchaikul P, Teanpaisan R. Effect of fermented milk containing Lactobacillus rhamnosus SD11 on oral microbiota of healthy volunteers: a randomized clinical trial. J Dairy Sci 2017; 100(10): 7780-7. doi:10.3168/jds.2017-12961.
Kadam P, Bhalerao S. Sample size calculation. Int J Ayurveda Res 2010; 1(1): 55-7. doi:10.4103/0974-7788.59946.
Shi J, Wang Q, Ruan G, Chen Y, Zhao M, Shi D, et al. Efficacy of probiotics against dental caries in children: a systematic review and meta-analysis. Crit Rev Food Sci Nutr 2022; 1: 1-18. doi:10.1080/10408398.2022.2077693.
McFarland LV, Evans CT, Goldstein EJC. Strain-specificity and disease-specificity of probiotic efficacy: a systematic review and meta-analysis. Front Med 2018; 5: 1-14. doi: 10.3389/fmed.2018.00124.
Näse L, Hatakka K, Savilahti E, Saxelin M, Pönkä A, Poussa T, et al. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res 2001; 35(6): 412-20. doi:10.1159/ 000047484.
Glavina D, Gorseta K, Skrinjarić I, Vranić DN, Mehulić K, Kozul K. Effect of LGG yoghurt on Streptococcus mutans and Lactobacillus spp. salivary counts in children. Coll Antropol 2012; 36(1): 129-32.
Rodríguez G, Ruiz B, Faleiros S, Vistoso A, Marró ML, Sánchez J, et al. Probiotic compared with standard milk for high-caries children: a cluster randomized trial. J Dent Res 2016; 95(4): 402-7. doi:10.1177/0022034 515623935.
Sandoval F, Faleiros S, Cabello R, Díaz-Dosque M, Rodríguez G, Escobar A. The consumption of milk supplemented with probiotics decreases the occurrence of caries and the salivary concentration of hβD-3 in children. Clin Oral Investig 2021; 25(6): 3823-30. doi:10.1007/s00784-020-03712-8.
Angarita-Díaz MP, Forero-Escobar D, Cerón-Bastidas XA, Cisneros-Hidalgo CA, Dávila-Narvaez F, Bedoya-Correa CM, et al. Effects of a functional food supplemented with probiotics on biological factors related to dental caries in children: a pilot study. Eur Arch Paediatr Dent 2020; 21(1): 161-9. doi: 10.1007/s40368-019-00468-y.
Villavicencio J, Villegas LM, Arango MC, Arias S, Triana F. Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. J Appl Oral Sci Rev 2018; 26: 1-9. doi:1 0.1590/1678-7757-2017-0318.
World Health Organization, Food and Agriculture Organization of the United Nations. Guidelines for the evaluation of probiotics in food. [online] 1 May 2002 [cited 2019 Jun 19]; Available form: URL:http://fanus.com.ar/posgrado/10-09-25/fao%20probiotics.pdf
Petti S, Tarsitani G, D’Arca AS. A randomized clinical trial of the effect of yoghurt on the human salivary microflora. Arch Oral Biol 2001; 46(8): 705-12. doi:10.1016/s0003-9969(01)00033-4.
Wannun P, Piwat S, Teanpaisan R. Purification, characterization, and optimum conditions of fermencin SD11, a bacteriocin produced by human orally Lactobacillus fermentum SD11. Appl Biochem Biotechnol 2016; 179(4): 572-82. doi:10.1007/s12010-016-2014-y.
Saetang P, Teanpaisan R, Piwat S. In vitro the impact of surface components and environmental factors on the adhesion and aggregation ability of oral Lactobacillus fermentum. J Dent Assoc Thai 2021; 71(3): 176-83. doi:10.14456/jdat.2021.19.
Caglar E, Topcuoglu N, Cildir SK, Sandalli N, Kulekci G. Oral colonization by Lactobacillus reuteri ATCC 55730 after exposure to probiotics. Int J Paediatr Dent 2009; 19(5): 377-81. doi: 10.1111/j.1365-263X.2009.00989.x.
Ravn I, Dige I, Meyer RL, Nyvad B. Colonization of the oral cavity by probiotic bacteria. Caries Res 2012; 46(2): 107-12. doi:10.1159/000336960.
Janczarek M, Bachanek T, Mazur E, Chałas R. The role of probiotics in prevention of oral diseases. Postepy Hig Med Dosw 2016; 70: 850-7. doi:10.5604/17322693.1214381.
Piwat S, Teanpaisan R, Manmontri C, Wattanarat O, Pahumunto N, Makeudom A, et al. Efficacy of probiotic milk for caries regression in preschool children: a multicenter randomized controlled trial. Caries Res 2020; 54(5-6): 491-501. doi:10.115 9/000509926.
Dettori JR. Loss to follow-up. Evid Based Spine Care J 2011; 2(1): 7-10. doi:10.1055/s-0030-1267080.
Pahumunto N, Piwat S, Chankanka O, Akkarachaneeyakorn N, Rangsitsathian K, Teanpaisan R. Reducing mutans streptococci and caries development by Lactobacillus paracasei SD1 in preschool children: a randomized placebo-controlled trial. Acta Odontol Scand 2018; 76(5): 331-7. doi:10.10 80/00016357.2018.1453083.
Liong MT. Safety of probiotics: translocation and infection. Nutr Rev 2008; 66(4): 192-202. doi:10.1111/j.1753-4887.2008.00024.x.