Evaluation of Liver and Kidney Toxicity in Rats Receiving Proteoglycans from Fish Cartilage for the Acceleration of Burn Wound Healing

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Published: Feb 19, 2016
DOI: https://doi.org/10.31524/bkkmedj.2016.02.003
Keywords:
proteoglycans, toxicity, liver, kidney, fish cartilage, burn wounds

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Sitthiphon Bunman, MSc
Narongpon Dumavibhat, MD, PhD
Noppadol Larbcharoensub, MD

Abstract

OBJECTIVE: The aim of this study was to evaluate the toxicity of proteoglycan extracted from fish cartilage for the acceleration of wound healing.

MATERIAL AND METHODS: Second degree burn wounds were induced by an electrical hot plate measuring 2 cm in diameter and set at a temperature of 90 oC and placed on the back of rats for 10 seconds. Rats were randomly assigned to receive 1 gram of cream base (control group), 1% silver sulfadiazine (SSD), 1% proteoglycans (PG), 2% PG, a combination of 1% SSD + 1% PG, or a combination of 1% SSD + 2% PG applied to burn wounds to accelerate wound healing immediately after burning and once daily until day 27 post-burn. The toxicity of the rats’ liver and kidney functions were evaluated on day 7, 14, 21 and 28.

RESULTS: An evaluation of liver function tests showed on day 28 that the level of aspartate aminotransferase, alanine transaminase, and alkaline phosphatase of rats treated with 1% SSD, 1% PG, 2% PG, combination of 1% SSD + 1% PG and combination of 1% SSD + 2% PG were not signifi cantly different when compared to the control group. An evaluation of blood urea nitrogen and creatinine levels, on day 28 showed the level of blood urea nitrogen and creatinine of rats treated with 1% SSD, 1% PG, 2% PG, combination of 1% SSD + 1% PG and combination of 1% SSD + 2% PG were not signifi cantly different when compared to the control group.

CONCLUSION: This study demonstrated that PG extracted from fish

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How to Cite
1.
Bunman S, Dumavibhat N, Larbcharoensub N. Evaluation of Liver and Kidney Toxicity in Rats Receiving Proteoglycans from Fish Cartilage for the Acceleration of Burn Wound Healing. BKK Med J [Internet]. 2016 Feb. 19 [cited 2024 Apr. 27];11(1):11. Available from: https://he02.tci-thaijo.org/index.php/bkkmedj/article/view/221407
Issue
Vol. 11 (2016): February
Section
Original Article

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References

1. Hettiaratchy S, and Dziewulski P. ABC of burns Introduction. BMJ 2004;328:1366-68.
2. Bunman S, Aramwit P, Larbcharoensub N, et al. Applica- tion of proteoglycans from fish cartilage for the accelera- tion of burn wound healing. TJPS 2015;39:64-9.
3. Cameron AM, Burn wound management: a surgical perspective. Wound Practice & Researc 2010;18:35-40.
4. Atiyeh BS, Hayek SN, and Gunn SW. New technologies for burn wound closure and healing-review of the literature. Burns 2005;31:944-56.
5. Atiyeh BS, Costagliola M, Hayek SN et al. Effect of silver on burn wound infection control and healing: review of the literature. Burns 2007;33:139-48.
6. Baldi C, Minoia C, Di Nucci A, et al. Effects of silver in isolated rat hepatocytes. Toxicol Lett 1988;41:261-68.
7. Chaby G, Viseux V, Poulain JF, et al. Topical silver sulfadiazine-induced acute renal failure. Ann Dermatol Venereol 2005;132:891-93.
8. Trop M, Novak M, Rodl S, et al. Silver-coated dressing acticoat caused raised liver enzymes and argyria-like symptoms in burn patient. J Trauma 2006;60:648-52.
9. Prydz K, Dalen KT, Synthesis and sorting of proteo glycans. J Cell Sci 2000;113:193-205.
10. Hardingham TE, and Fosang AJ, Proteoglycans: many forms and many functions. FASEB J 1992;6:861-70.
11. Perrimon N, and Bernfield M, Cellular functions of proteoglycans an overview. Semin. Cell Dev. Biol. 2001; 12:65-7.
12. Hardingham TE, and Fosang AJ, Proteoglycans: many forms and many functions. FASEB J 1992;6:861-70.
13. Neelam B, Titpawan N, and Teerapol, In vitro stimulatory effect of grandiflorenic acid isolated from Wedelia trilobata (L.) leaves on L929 fibroblast cells. TJPS 2013; 37:117-24.
14. Raab G, and Klagsbrun M, Heparin-binding EGF-like growth factor. Biochim Biophys Acta 1997;1333:179-99.
15. Somboonwong J, Kankaisre M, Tantisira B, et al. Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: an experimental animal study. BMC Complement Altern. Med. 2012;12:103.
16. Zawacki BE, The natural history of reversible burn injury. Surg. Gynecol. Obstet. 1974;139:867-72.
17. Diehl KH, Hull R, Morton D, et al. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol 2001; 21:15-23.
18. Koch M, Experimental modeling and research Methodology. The Laboratory Rat, 2nd ed. Suckow MA, Weisbroth SH, and Franklin CL (Eds.), Burlington, MA: Elsevier Academic Press. 2006;593-94.
19. Henry JB. Clinical diagnosis and management by labora- tory methods. 19th ed. Philadelphia : Saunders, 1996:1928- 2009.
20. Thavasu PW, Longhurst S, Joel SP, et al. Measuring cytokine levels in blood. Importance of anticoagulants, processing, and storage conditions. J Immunol Methods 1992;153:115-24.
21. Kanai S, Honda T, Uehara T, et al. Liver function tests in patients with bacteremia. J Clin Lab Anal 2008;22:66-9.
22. Diloke B, Mathurot D, Rapee I, et al. Chemical parameters in healthy Sprague-Dawley and Wistar rats from National Laboratory Animal Center, Mahidol University, 2011.
23. Salama SM, Bilgen M, Al Rashdi AS, et al. Efficacy of Boesenbergia rotunda treatment against thioacetamide- induced liver cirrhosis in a rat model. eCAM 2012.
24. Knudson CB, and Knudson W, Cartilage proteoglycans.Semin Cell Dev Biol 2001;12:69-78.
25. Thrall, Mary A, Weiser, et al. Veterinary hematology and clinical chemistry: John Wiley & Sons. 2012.
26. Zhang, Zong-Peng, Tian, et al. The comparison of the normal blood biochemical values of Wistar rats with different age and sex. Drug Metab Pharmacokinet 2004; 4:215-8.