Mass Spectrometry-based Serum Proteome Pattern Analysis in Prophylactic Oophorectomy

Abstract

Objective: To study role of ovary reflecting from serum proteomic pattern changes before and after prophylactic oophorectomy

Materials and Methods: A prospective cohort study with 20 premenopausal women diagnosed benign gynecologic condition but not ovary and scheduled for prophylactic oophorectomy was conducted. Blood was drawn and serum was centrifuged and kept at -80 degree Celsius before and at 6 weeks after oophorectomy. All serum specimens were prepared and then analyzed by MALDI-TOF.

Results: Age, BMI and duration of follow up were 49.30±1.59 years, 24.56±4.07 kg/m² and 45.70±6.13 days, respectively. A comparison of mass spectrum intensity between before and after oophorectomy showed that five m/z 1882, 2467, 8766, 8923 and 9316 were statistically significant different (7.96 and 16.93, P < 0.001, 2.42 and 8.57, P = 0.01, 4.12 and 6.04, P =0.005, 16.06 and 18.40, P =0.002, 8.54 and 12.59 P =0.016 , respectively).

Conclusion: MS revealed a significant change of serum proteomic pattern from women undergone prophylactic oophorectomy. The protein identification study is currently ongoing in order to identify the ovarian function.