Comparison of Sperm Motility and Sperm Recovery Rate After 40 %/80% and 50%/100% Discontinuous Percoll Gradients Preparation

Abstract

Two discontinuous Percoll gradients (40%/80% and 50%/100% Percoll gradients) were assessed for efficacy of selection of motile sperm from 55 normozoospermic semen samples. Each semen sample was assessed for basic semen parameters before and after preparation by both techniques. The prepara tion of sperm on both two step Percoll gradients allowed about one third of the progressively motile sperm to be recovered. Sperms prepared by the 50%/100% Percoll gradients had higher percent progressive motility and percent normal morphology but there were no statistically significant differences between both techniques. Sperm preparation by 40%/80% Percoll gradients seems to be better than 50%/100% Percoll gradients because total sperm number, total motile sperm number, and total sperm recovery rate after preparation by 40%/80% Percoll gradients were greater than 50%/100% Percoll gradients significantly. (Thai J Obstet Gynaecol 1995;7:25-32.)