In Vitro Fertilization of Human In vitro Matured Oocytes : Report of the First 22 Cases in Thailand

Abstract

This is the first report in Thailand, of the success of in vitro matu ration (IVM) of human oocytes and in vitro fertilization (IVF) of those IVM-eggs. Preliminary results from a study period of 6 months showed that, from 22 patients, forty-nine immature oocytes were obtained via laparotomy or laparoscopy. About half (24/49) of those oocytes matured in vitro, two-thirds (16/24) of which were fertilizable. Among those fertilized eggs, nearly half (7/16) of them normally fertilized, and the remainders (9/16) ended up with polyspermic fertilization (>2 pronuclei). A number of normally cleaved embryos of 2- to 8-cell stage were transferred, while some of them were further cultured in vitro. Eventhough a few expanded blastocysts were obtained in vitro, none of the transferred embryos implanted successfully. Detailed materials and methods are described, and the results are compared to those of standard IVF. Physiology of normal and abnormal fertilization of IVM-oocytes are also discussed. (Thai J Obstet Gynaecol 1994; 6: 71-78.)