Preliminary Experiences with IVF & ET at Maharaj Nakorn Chiang Mai Hospital

Abstract

This is the first report of 35 patients who registered for IVF-ET at Ma haraj Nakorn Chiang Mai Hospital from January 1 to September 30, 1991. The patients' ages ranged from 29-42 years, with a duration of infertility of 2-16 years. Gonadotropin releasing hormone agonist and human menopausal gonadotropins were prescribed for superovulation. Follicular growth was monitored by serial measurement of serum estradiol levels and pelvic ultrasound. Human chorionic go nadotropin was given intramuscularly when sonogram demostrated two or more fol licles 2 17 mm in diameter. GnRH-a was discontinued and oocyte retrieval was performed 34-36 hours later. Sperm was prepared by the washing and swim-up technique. Mature oocyte was inseminated with 100000 motile sperms and was ob served for evidence of fertilization 12-16 hours later. Embryo transfer was per formed 48-72 hours after oocyte was retrieval. 

Of the 336 harvested oocytes, 183 (55.3%) achieved fertilization. Embryo transfer was done in 37 cycles at 2- to 8-cell stages and resulted in 4 clinical preg nancies (10.8% of ET). Two pregnancies ended in spontaneous miscarriages at 11 weeks of gestation, the other two are well on-going. (Thai J Obstet Gynaecol 1992;4: 23-32.)