The Effects of Autologous Platelet-rich Plasma Supplement during Sperm Cryopreservation on Post-cryopreserved Sperm Quality
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Abstract
Objective: This study aimed to evaluate human sperm vitality after cryopreservation by comparing a cryopreservation medium with and without the addition of autologous platelet-rich plasma (aPRP).
Methods: Semen samples were collected from normozoospermic men. Each sample was separated into two tubes: one without aPRP supplementation and one with 5% aPRP supplementation. Both tubes were cryopreserved for 14 days. Sperm parameters, including sperm concentration, motility, morphology, vitality, and DNA fragmentation index (DFI), were measured and analyzed.
Results: Fifteen semen samples were included. After cryopreservation, sperm vitality was significantly higher in specimens with aPRP supplementation compared to those without: 32.13% ±12.02% versus 26.07% ±9.30%, respectively (p=0.009). There were no significant differences between the two groups in other sperm parameters, including sperm concentration: 12.73x106/mL (IQR 21.95 x106/mL) versus 14.50x106/mL (IQR 25.31x106/mL) (p=0.053); morphology: 4.40% ±SD 0.98% versus 4.40% ±SD 1.18% (p>0.999); total motility: 8.73% (IQR 9.16%) versus 8.56% (IQR 11.24%) (p=0.410); progressive motility: 5.67% (IQR 8.66%) versus 6.61% (IQR 6.35%) (p=0.887), and DFI: 12.00% (IQR 15%) versus 17.00% (IQR 26%) (p=0.139).
Conclusions: The supplementation of cryopreservation media with aPRP significantly increased sperm survival rates after the freeze-thaw process. These findings suggest that aPRP may be an effective adjunct in cryopreservation protocols to improve sperm viability. The impact of combined additional high-efficiency cryoprotectants on relevant reproductive outcomes, including fertilization, blastocyst formation, and pregnancy rates, needs further investigation.
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