The Effects of Autologous Platelet-rich Plasma Supplement during Sperm Cryopreservation on Post-cryopreserved Sperm Quality
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Abstract
Objectives: This study aimed to evaluate human sperm vitality after cryopreservation by comparing a cryopreservation medium with and without the addition of autologous platelet-rich plasma (aPRP).
Materials and Methods: Semen samples were collected from normozoospermic men. Each sample was separated into two tubes: one without aPRP supplementation and one with 5% aPRP supplementation. Both tubes were cryopreserved for 14 days. Sperm parameters, including sperm concentration, motility, morphology, vitality, and deoxyribonucleic acid (DNA) fragmentation index (DFI), were measured and analyzed.
Results: Fifteen semen samples were included. After cryopreservation, sperm vitality was significantly higher in specimens with aPRP supplementation compared to those without (32.13% ± 12.02% vs 26.07% ± 9.30%, respectively, p = 0.009). There were no significant differences between the two groups in other sperm parameters, including sperm concentration (12.73 x 106/mL (interquartile range (IQR) 21.95 x 106/mL) vs 14.50 x 106/mL (IQR 25.31 x 106/mL), p = 0.053), morphology (4.40% ± standard deviation (SD) 0.98% vs 4.40% ± SD 1.18%, p > 0.999), total motility (8.73% (IQR 9.16%) vs 8.56% (IQR 11.24%), p = 0.410), progressive motility (5.67% (IQR 8.66%) vs 6.61% (IQR 6.35%), p = 0.887), and DFI (12.00% (IQR 15%) vs 17.00% (IQR 26%), p = 0.139).
Conclusion: The supplementation of cryopreservation media with aPRP significantly increased sperm survival rates after the freeze-thaw process. These findings suggest that aPRP may be an effective adjunct in cryopreservation protocols to improve sperm viability. The impact of combined additional high efficiency cryoprotectants on relevant reproductive outcomes, including fertilization, blastocyst formation, and pregnancy rates, needs further investigation.
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