Performance of test kit for antimicrobial residue in meats using microbial inhibition assay

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Warangkhana Chaisowwong
Chuleeporn Saksangawong
Ravisa Warin
Ratchadaporn Boripun
Jamras Lerdsri
Montira Intanon


Antimicrobial residue in meats causes negative effects to consumer especially antimicrobial resistance which is public health problem worldwide. The research aims to study a performance of the test kit that has been developed using microbial inhibition assay. The test kit had Limit of Detection (LOD) for tetracyclines at 400 μg/kg. One hundred-fifty meat samples that sold in Chiang Mai province were tested and 8 (5.33%) samples showed positive result which was concordant to CM®test and discordant to Premi®test. Drug concentration of positive samples were analysed using High performance liquid chromatography. Two samples were found a combination of drug residues including oxytetracycline with chlortetracycline and tetracycline with oxytetracycline at 141, 128, 154, and 145 μg/kg respectively. The test kit can be applied for screening of tetracyclines in meats under the limit of detection as mentioned. It is not expensive and do not require a complicated protocol. Furthermore, this study showed a situation of antimicrobial residue in meats which identified risk to human and needs recognition and collaboration from every stakeholder for solutions.


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Chaisowwong, W., Saksangawong, C., Warin, R., Boripun, R., Lerdsri, J., & Intanon, M. (2018). Performance of test kit for antimicrobial residue in meats using microbial inhibition assay. Veterinary Integrative Sciences, 16(1), 27–36. Retrieved from
Research Articles


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