Green synthesized copper nanoparticles and their anti-bacterial properties against bullfrog multidrug resistant gram negative bacteria
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Published:
Apr 17, 2019
Keywords:
antioxidant; copper nanoparticles; bullfrog; multidrug resistance; antibacterial susceptibility
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Abstract
The emergence of multidrug-resistant (MDR) gram-negative bacteria in bullfrog (Hoplobatrachus rugulosus) farming has been increasing dramatically resulted in searching for new types of antimicrobial agents. Although some new drugs against MDR bacteria have been introduced or presently in clinical trials, the efficiency of them are still limited by species of pathogen. Therefore, copper nanoparticles (CuNPs) has been immerged in MDR treatment due to their greater exhibition in broad-spectrum bactericidal properties. To prepare green synthesized CuNPs, the high antioxidant property of plant aqueous extracts assessed by scavenging free radicals of DPPH and Reducing Power (RP) of Ferric acid were used. It was indicated that the IC50 value of extract was greatest in Garcinia mangostana following Camellia sinensis, Phyllanthus urinaria, P. amarus and P. virgatus, respectively ranging from 226.59±9.27 to 487.35±6.31 (ug/mL) and positive related to RP antioxidant activities. The formation CuNPs were characterized using UV-visible spectroscopy revealed a maximum absorbance at 340 nm. CuNPs using G. mangostana (GM-CuNPs) exhibited the greatest significant bactericidal activity against multi-drug resistant gram negative bacterial strains such as Aeromonase sorbia, Edwardsiella tarda, Enterobacter spp., Klebsiella pneumoniae, and Pseudomonas spp. by agar well diffusion method. Moreover, the results from Dynamic light scattering (DLS) demonstrated that only size of GM-CuNPs was in the nano-size range of 254±144.9 nm whereas the zeta potential was in the range of -0.37±11.3 mV. It can be concluded that GM-CuNPs exhibit greatest antibacterial properties for MDR treatment and should be candidate for future bullfrog MDR therapeutic application.
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Sassa-deepaeng, tanongsak, Yodthong, W., & khamphira, T. (2019). Green synthesized copper nanoparticles and their anti-bacterial properties against bullfrog multidrug resistant gram negative bacteria. Veterinary Integrative Sciences, 17(1), 33–49. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/136649
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References
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Lutsenko, S., Barnes, N.L., Bartee, M.Y., Dmitriev O.Y., 2007. Function and Regulation of Human Copper-Transporting ATPases. Physiol. Rev. 87 (3), 1011-1046.
Lv, H., Zhang Y., Shi, J., Lin, Z., 2017. Phytochemical profiles and antioxidant activities of Chinese dark teas obtained by different processing technologies. Food Res. Int. 100 (3), 486-493.
Mandava, K., Kadimcharla, K., Keesara, N.R., Fatima, S.N., Bommena, P., Batchu, U.R., 2017. Green Synthesis of Stable Copper Nanoparticles and Synergistic Activity with Antibiotics. Indian J. Pharm. Sci. 79(5), 695-700.
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Mathur, A., Kushwaha, A., Dalakoti, V., Dalakoti, G., Singh, D.S., 2014. Green synthesis of silver nanoparticles using medicinal plant and its characterization, Pharm. Lett. 5, 118-122.
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Milugo, T.K., Omosa, L.K., Owuor, B., Ochanda, J.O., Ochieng J. W., 2013. Antagonistic effect of alkaloids and saponins on bioactivity in the quinine tree (Rauvolfia caffra sond.): further evidence to support biotechnology in traditional medicinal plants. BioMed Central. 13 (285) doi: 10.1186/1472-6882-13-285.
Murphy, C.J., Jana, N.R., 2002. Controlling the aspect ratio of inorganic nanorods and nanowires. Adv. Mater. 14 (1), 80-82.
Naika, H.R., Lingaraju, K., Manjunath, K., Kumar, D., Nagaraju, G., Suresh, D., Nagabhushana, H., 2015. Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity. Journal of Taibah University for Science. 9, 7-12.
Navarro, M., Moreira, I., Arnaez, E., Quesada, S., Azofeifa, G., Vargas, F., Alvarado, D., Chen, P., 2017. Flavonoids and Ellagitannins Characterization, Antioxidant and Cytotoxic Activities of Phyllanthus acuminatus Vahl. Plants (Basel). 6(4). doi: 10.3390/plants6040062
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Poompachee, K., Chudapongse, N., 2012. Comparison of the Antioxidant and Cytotoxic Activities of Phyllanthus virgatus and Phyllanthus amarus Extracts. Med. Princ. Pract. 21 (1), 24-29.
Rajesh, K.M., Ajitha, B., Reddy, Y.A.K, Suneetha, Y., Reddy, P.S.Z., 2018. Assisted green synthesis of copper nanoparticles using Syzygium aromaticum bud extract: Physical, optical and antimicrobial properties. Optik. 154, 593-600.
Sassa-Deepaeng, T., Khamphira, T., Yodthong, W., Wanapichit, V., 2017. Antibacterial and Antioxidant composition of Eclipta prostrata. Prodeeding book 4th CRCI & 2nd ISHPMNB 2017.
Sassa-deepaeng, Pikulkaew, S., Okonogi, S., 2016. Development of chrysin loaded poloxamer micelles and toxicity evaluation in fish embryos. 10(3), 150-155.
Selvan, D.A., Mahendiran, D., Kumar, R.S., Rahiman, A.K., 2018. Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: Phytochemical, antioxidant and in vitro cytotoxicity studies. J. Photochem. Photobiol. B: Biology 180, 243-252.
Shikha, J.A., Ankita, J.A., Kachhawah, P., Devra, V., 2015. Synthesis and size control of copper nanoparticles and their catalytic application. Trans Nonferrous Met. Soc. China. 25(12), 3995-4000.
Siddiqui, A.A., Ali, M., 1997. Practical Pharmaceutical Chemistry,1st ed., CBS Publishers
Singh, K., Panghal, M., Kadyan, S., Chaudhary, U., Yadav, J.P., 2014. Green silver nanoparticles of Phyllanthus amarus: as an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa. Journal of nanobiotechnology, 12(1), 40.
Sua´rez-Cerda, J., Espinoza-Go´mez, H., Alonso-Nu´n˜ez, G., Rivero, I. A., Gochi-Ponce, Y., Flores-Lo´pez, L.Z., 2017. A green synthesis of copper nanoparticles using native cyclodextrins as stabilizing agents. Journal of Saudi Chemical Society. 21, 341-348.
Tanwar, J., Das, S., Fatima, Z., Hameed, S., 2014. Multidrug Resistance: An Emerging Crisis. Hindawi Publishing Corporation Interdisciplinary Perspectives on Infectious Diseases. Article ID 541340, https://dx.doi.org/10.1155/2014/541340
Veerasamy, R., Xin, T.Z., Gunasagaran, S., Xiang, T.F.W., Yang, E.F.C., Jeyakumar, N., Dhanaraj, S.A., 2011. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society, 15(2), 113120.
WHO.,2017. https://www.who.int/mediacentre/news/releases/2017/bacteria-antibiotics-needed/en/ (accessed 13 December 2017).
Yadav, L., Tripathi, R.M., Prasad, R., Pudake, R.N., Mittal, J. 2017. Antibacterial Activity of Cu Nanoparticles against E. coli, Staphylococcus aureus and Pseudomonas aeruginosa. Nano Biomed. Eng. 9(1), 9-14.
Yoon, K., Byeon, H.J., Park, J.H. and Hwang, J., 2007. Susceptibility Constants of Escherichia coli and Bacillus subtilis to Silver and Copper Nanoparticles. Science of the Total Environment, 373, 572-575.
Zengin, H., Baysal, A. H., 2014. Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy. Molecules. 19(11), 17773-98.
Zhang A, Fang Y, Wang H, Li H, Zhang Z. 2011. Free-radical scavenging properties and reducing power of grape cane extracts from 11 selected grape cultivars widely grown in china. Molecules. 16, 10104–10122. doi: 10.3390/molecules161210104.
Ahmad, A., Singh, D.K., Fatima, K., Tandon, S., Luqman, S., 2014. New constituents from the roots of Oenothera biennis and their free radical scavenging and ferric reducing activity. Ind Crop Prod, 58, 125-132
Ajitha, B., Reddy, Y.A.K., Jeon, H.J., Ahn, C.W., 2018. Synthesis of silver nanoparticles in an eco-friendly way using Phyllanthus amarus leaf extract: Antimicrobial and catalytic activity. Advanced Powder Technology. 29, 86-93.
Alsultan, Q.M.N., Sijam, K., Rashid, T.S., Ahmad, K.B., Awla, H.K., 2017. Investigation of Phytochemical Components and Bioautography of Garcinia mangostana L. Methanol Leaf Extract. Journal of Experimental Agriculture International 15 (3), 1-7.
Ashtaputrey, S.D., Ashtaputrey, P.D., Yelane, N., 2017. Green synthesis and characterization of copper nanoparticles derived from Murraya koenigii leaves extract, J. Chem. Pharm. Sci. 10 (3), 1288-1291.
Bargah, R.K., 2015. Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of Moringa pterygosperma Gaertn. Journal of Pharmacognosy and Phytochemistry 4(1), 07-09.
Brand-Williams, W., Cuvelier, M.E., Berset, C., 1995. Use of free radical method to evaluate antioxidant activity. LWT- Food Science and Technology 28 (1), 25-30.
Buranasinsup, S., Kulpeanprasit, S., Kong-ngoen, T., Jangsangthong, A., Sookrung, N., Chaicumpa, W., and Indrawattana, N., 2018. Prevelence of the Multi-drug Resistance of Shiga Toxin-producting Escherichia coli Isolated from Pigs in Central Thailand. Chiang Mai J. Sci. 45(1), 21-32.
Chang C.C., Yang, M.H., Wen H.M., Chern, J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 10, 178-182.
Chung, I.M., Rahuman, A.A., Sampath, M., Kirthi, A.V., Anbarasan, K., Parthasarathy, P. Rajakumar, G., 2017. Green synthesis of copper nanoparticles using Eclipta prostrata leaves extract and their antioxidant and cytotoxic activities. Exp Ther Med. 14, 18-24.
Clinical and Laboratory Standards Institute CLSI., 2012. Performance standards for antimicrobial susceptibility testing: twenty-two informational supplement. CLSI Document M 100-S22. CLSI, Wayne, PA. 32.
Chitra, K., Reena, K., Manikandan, A., Antony, S.A., 2015. Antibacterial Studies and Effect of Poloxamer on Gold Nanoparticles by Zingiber Officinale Extracted Green Synthesis. J Nanosci Nanotechnol, 15(7), 4984-4991.
DeAlba-Montero, I., Guajardo-Pacheco, J., Morales-Sánchez, E., Araujo-Martínez, R., Loredo-Becerra, G. M., Martínez-Castañón, G.A., Ruiz, F., Compeán Jasso M. E., 2017. Antimicrobial properties of copper nanoparticles and amino acid chelated copper nanoparticles produced by using a soya extract. Bioinorg Chem Appl. 2017:1064918. doi: 10.1155/2017/1064918.
Falcão, L. and Araújo, M.E.M., 2011. Tannins Characterisation in New and Historic Vegetable Tanned Leather Fibres by Spot Tests. Journal of Cultural Heritage 12(2),149-156.
Gibbs, E.L., Gibbs, T.J., Van Dyck, P.C., 1966. Rana pipiens: Health and disease. Lab. Anim. Care 16, 142-160.
Glorioso, J.C., Amborski, R.L., Amborski, G.F., Culley, D.D., 1974. Microbiological studies on septicemic bullfrogs (Rana catesbeiana). Am. J. Vet. Res. 35, 1241-1245.
Green, S. L, Bouley, D.M., Tolwani, R.J., Waggie, K.S., Lifland. B.D., Otto. G.M., Ferrell, J.E. Jr., 1999. Identification and management of an outbreak of Flavobacterium meningosepticum infection in a colony of South African clawed frogs. (Xenopus laevis). J Am Vet Med Assoc. 214, 1833–1838.
Harborne, J.B., 1973. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd ed., Chapman and Hall Ltd, London.
Hedayatianfard, K., Akhlaghi, M., and Sharifiyazdi, H., 2014. Detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction. Vet Res Forum. Autumn; 5(4): 269–275.
Lv, J., Wang, Y., Zhang, L., Lin, H., Zhao, J., Ma, Y., 2012. Stabilization of Fullerene-like Boron Cages by Transition Metal Encapsulation. Journal of Nanoscale. DOI: 10.1039/C5NR01659B.
Li, Y.F., Ouyang, S. H., Chang, Y.Q., Wang, T.M., Li, W. X., Tian, H. Y., Cao, H., Kurihara, H., He, R. R., 2017. A comparative analysis of chemical compositions in Camellia sinensis var. puanensis Kurihara, a novel Chinese tea, by HPLC and UFLC-Q-TOF-MS/MS. Food Chemistry. 216, 282-288.
Lutsenko, S., Barnes, N.L., Bartee, M.Y., Dmitriev O.Y., 2007. Function and Regulation of Human Copper-Transporting ATPases. Physiol. Rev. 87 (3), 1011-1046.
Lv, H., Zhang Y., Shi, J., Lin, Z., 2017. Phytochemical profiles and antioxidant activities of Chinese dark teas obtained by different processing technologies. Food Res. Int. 100 (3), 486-493.
Mandava, K., Kadimcharla, K., Keesara, N.R., Fatima, S.N., Bommena, P., Batchu, U.R., 2017. Green Synthesis of Stable Copper Nanoparticles and Synergistic Activity with Antibiotics. Indian J. Pharm. Sci. 79(5), 695-700.
Mao, X., Wu, L.F, Guo, H.L., Chen, W.J., Cui, Y.P., Qi, Q., Li, S., Liang, W.Y., Yang, G.H., Shao, Y.Y., Zhu, D., She, G.M., You, Y., Zhang, L.Z., 2016. The Genus Phyllanthus: An Ethnopharmacological, Phytochemical, and Pharmacological Review. Evid Based Complement Alternat Med. Article ID 7584952, https://dx.doi.org/10.1155/2016/7584952
Mathur, A., Kushwaha, A., Dalakoti, V., Dalakoti, G., Singh, D.S., 2014. Green synthesis of silver nanoparticles using medicinal plant and its characterization, Pharm. Lett. 5, 118-122.
Mauel, M.J., Miller, D.L., Frazier, K.S., Hines, M.E., 2002. Bacterial pathogens isolated from cultured bullfrogs (Rana catesbeiana) . J Vet Diag Invest. 14, 431-433.
Milugo, T.K., Omosa, L.K., Owuor, B., Ochanda, J.O., Ochieng J. W., 2013. Antagonistic effect of alkaloids and saponins on bioactivity in the quinine tree (Rauvolfia caffra sond.): further evidence to support biotechnology in traditional medicinal plants. BioMed Central. 13 (285) doi: 10.1186/1472-6882-13-285.
Murphy, C.J., Jana, N.R., 2002. Controlling the aspect ratio of inorganic nanorods and nanowires. Adv. Mater. 14 (1), 80-82.
Naika, H.R., Lingaraju, K., Manjunath, K., Kumar, D., Nagaraju, G., Suresh, D., Nagabhushana, H., 2015. Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity. Journal of Taibah University for Science. 9, 7-12.
Navarro, M., Moreira, I., Arnaez, E., Quesada, S., Azofeifa, G., Vargas, F., Alvarado, D., Chen, P., 2017. Flavonoids and Ellagitannins Characterization, Antioxidant and Cytotoxic Activities of Phyllanthus acuminatus Vahl. Plants (Basel). 6(4). doi: 10.3390/plants6040062
Omara, S.T., 2017. MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis. Journal of Genetic Engineering and Biotechnology 15, 219-230.
Poompachee, K., Chudapongse, N., 2012. Comparison of the Antioxidant and Cytotoxic Activities of Phyllanthus virgatus and Phyllanthus amarus Extracts. Med. Princ. Pract. 21 (1), 24-29.
Rajesh, K.M., Ajitha, B., Reddy, Y.A.K, Suneetha, Y., Reddy, P.S.Z., 2018. Assisted green synthesis of copper nanoparticles using Syzygium aromaticum bud extract: Physical, optical and antimicrobial properties. Optik. 154, 593-600.
Sassa-Deepaeng, T., Khamphira, T., Yodthong, W., Wanapichit, V., 2017. Antibacterial and Antioxidant composition of Eclipta prostrata. Prodeeding book 4th CRCI & 2nd ISHPMNB 2017.
Sassa-deepaeng, Pikulkaew, S., Okonogi, S., 2016. Development of chrysin loaded poloxamer micelles and toxicity evaluation in fish embryos. 10(3), 150-155.
Selvan, D.A., Mahendiran, D., Kumar, R.S., Rahiman, A.K., 2018. Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: Phytochemical, antioxidant and in vitro cytotoxicity studies. J. Photochem. Photobiol. B: Biology 180, 243-252.
Shikha, J.A., Ankita, J.A., Kachhawah, P., Devra, V., 2015. Synthesis and size control of copper nanoparticles and their catalytic application. Trans Nonferrous Met. Soc. China. 25(12), 3995-4000.
Siddiqui, A.A., Ali, M., 1997. Practical Pharmaceutical Chemistry,1st ed., CBS Publishers
Singh, K., Panghal, M., Kadyan, S., Chaudhary, U., Yadav, J.P., 2014. Green silver nanoparticles of Phyllanthus amarus: as an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa. Journal of nanobiotechnology, 12(1), 40.
Sua´rez-Cerda, J., Espinoza-Go´mez, H., Alonso-Nu´n˜ez, G., Rivero, I. A., Gochi-Ponce, Y., Flores-Lo´pez, L.Z., 2017. A green synthesis of copper nanoparticles using native cyclodextrins as stabilizing agents. Journal of Saudi Chemical Society. 21, 341-348.
Tanwar, J., Das, S., Fatima, Z., Hameed, S., 2014. Multidrug Resistance: An Emerging Crisis. Hindawi Publishing Corporation Interdisciplinary Perspectives on Infectious Diseases. Article ID 541340, https://dx.doi.org/10.1155/2014/541340
Veerasamy, R., Xin, T.Z., Gunasagaran, S., Xiang, T.F.W., Yang, E.F.C., Jeyakumar, N., Dhanaraj, S.A., 2011. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society, 15(2), 113120.
WHO.,2017. https://www.who.int/mediacentre/news/releases/2017/bacteria-antibiotics-needed/en/ (accessed 13 December 2017).
Yadav, L., Tripathi, R.M., Prasad, R., Pudake, R.N., Mittal, J. 2017. Antibacterial Activity of Cu Nanoparticles against E. coli, Staphylococcus aureus and Pseudomonas aeruginosa. Nano Biomed. Eng. 9(1), 9-14.
Yoon, K., Byeon, H.J., Park, J.H. and Hwang, J., 2007. Susceptibility Constants of Escherichia coli and Bacillus subtilis to Silver and Copper Nanoparticles. Science of the Total Environment, 373, 572-575.
Zengin, H., Baysal, A. H., 2014. Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy. Molecules. 19(11), 17773-98.
Zhang A, Fang Y, Wang H, Li H, Zhang Z. 2011. Free-radical scavenging properties and reducing power of grape cane extracts from 11 selected grape cultivars widely grown in china. Molecules. 16, 10104–10122. doi: 10.3390/molecules161210104.