The distribution of streptococcus agalactiae in nile tilapia (Oreochromis niloticus) after oral inoculation
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Abstract
Streptococcus agalactiae is an important pathogen that causes severe mortality in the Nile tilapia fish within a short period of time. The objective of this study was to determine the distribution of S. agalactiae in fish organs at various intervals of time after oral inoculation in order to understand the systemic dissemination of S. agalactiae after inducing the fish with the disease. Thirty Nile tilapia with an average weight of 14 g were used in this study. Nile tilapia (N=27) were each orally infected with 0.3 ml of bacterial concentration of 5.92x108 CFU/ml and were maintained out of water for 5 min. Three fish were consecutively and randomly sampled within 30 min to 48 h. The control fish (N=3) were uninfected with S. agalactiae and sampled prior to S. agalactiae inoculation. All samples were investigated for S. agalactiae by bacteriological and polymerase chain reaction (PCR) techniques. S. agalactiae was recovered from the stomach, intestine, spleen, liver, kidneys and eyes after 30 min post inoculation but for the heart and brain, where were recovered S. agalactiae at 1 h post inoculation. All samples consistently recovered S. agalactiae by the end of the experiment, except the intestine and the eyes following 48 h of a period. PCR was performed on all sampled fish organs throughout the experiment. The results in this study demonstrated that the gastrointestinal tract successfully produced S. agalactiae infection that was then differently disseminated throughout important organs in the Nile tilapia.
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References
Abuseliana, A. F., Daud, H. H. M., Aziz, S. A., Bejo, S. K., & Alsaid, M., 2011. Pathogenicity of Streptococcus agalactiae Isolated from a Fish Farm in Selangor to Juvenile Red Tilapia (Oreochromis sp.). Journal of Animal and Veterinary Advances, 10(7), 914-919.
Abdullah, S., Omar, N., Yusoff, S. M., Obukwho, E. B., Nwunuji, T. P., Hanan, L., & Samad, J., 2013. Clinicopathological features and immunohistochemical detection of antigens in acute experimental Streptococcus agalactiae infection in red tilapia (Oreochromis spp.). Springerplus 2, 286.
Austin, B. & Austin, D.A., 2007. Bacterial Fish Pathogens. Diseases of Farmed and Wild Fish. 4th ed. Springer/Prazis Publishing, Chichester. 552 pp.
Azad, I.S., Al-Marzouk, A., James, C.M., Almatar, S., Al-Gharabally, H., Qasem, J.A., 2012.
Outbreak of natural Streptococcosis in hatchery produced silver pomfret (Pampus argenteus Euphrasen) larvae in Kuwait. Aquaculture 330-333, 15-20.
Bromage, E.S. & Owens, L., 2002. Infection of barramundi Lates calcarifer with Streptococcus iniae: effects of different routes of exposure. Diseases of aquatic organisms 52, 199-205.
Delamare-Deboutteville, J., Bowater, R., Condon, K., Reynolds, A., Fisk, A., Aviles, F., & Barnes, A. C., 2015. Infection and pathology in Queensland grouper, Epinephelus lanceolatus, (Bloch), caused by exposure to Streptococcus agalactiae via different routes. Journal of Fish Diseases 38(12), 1021-1035.
Eldar, A., Bejerano, Y., Livoff, A., Horovitcz, A. & Bercovier, H., 1995. Experimental streptococcal meningo-encephalitis in cultured fish. Veterinary Microbiology 43, 33-40.
Evans, J. J., Shoemaker, C. A., & Klesius, P. H., 2000. Experimental Streptococcus iniae infection of hybrid striped bass (Morone chrysops x Morone saxatilis) and tilapia (Oreochromis niloticus) by nares inoculation. Aquaculture 189, 197-210.
Evans, J. J., Shoemaker, C. A., & Klesius, P. H., 2001. Distribution of Streptococcus iniae in hybrid striped bass (Morone chrysops x Morone saxatilis) following nares inoculation. Aquaculture 194(3-4), 233-243.
Evans, J. J., Klesius, P. H., Glibert, P. M., Shoemaker, C. A., Al Sarawi, M. A., Landsberg, J. & Al Zenki, S., 2002. Characterization of beta-haemolytic Group B Streptococcus agalactiae in cultured seabream, Sparus auratus L., and wild mullet, Liza klunzingeri (Day), in Kuwait. Journal of Fish Diseases 25(9), 505-513.
Geng, Y., Wang, K. Y., Huang, X. L., Chen, D. F., Li, C. W., Ren, S. Y. & Lai, W. M., 2012. Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya-Fish, Schizothorax prenanti, in China. Transboundary and Emerging Diseases 59(4), 369-375.
Hernandez, E., Figueroa, J., & Iregui, C., 2009. Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study. Journal of Fish Diseases 32(3), 247-252.
Iregui, C. A., Comas, J., Vasquez, G. M., & Verjan, N., 2015. Experimental early pathogenesis of Streptococcus agalactiae infection in red tilapia (Oreochromis spp.) Journal of Fish Diseases 39(2), 205-215.
Klesius, P.H., Shoemaker, C.A. & Evans, J.J., 2000. Efficacy of single and combined Streptococcus iniae isolate vaccine administered by intraperitoneal and intramuscular routes in tilapia. Aquaculture 188, 237-246.
Laith, A. A., Ambak, M. A., Hassan, M., Sheriff, S. M., Nadirah, M., Draman, A. S. & Najiah, M., 2017. Molecular identification and histopathological study of natural Streptococcus agalactiae infection in hybrid tilapia (Oreochromis niloticus). Veterinary World 10(1), 101-111.
Li, Y. W., Liu, L., Huang, P. R., Fang, W., Luo, Z. P., Peng, H. L. & Li, A. X., 2014. Chronic streptococcosis in Nile tilapia, Oreochromis niloticus (L.), caused by Streptococcus agalactiae. Journal of Fish Diseases 37(8), 757-763.
McNulty, S.T., Klesius, P.H., Shoemaker, C.A. & Evans, J.J., 2003. Streptococcus iniae infection and tissue distribution in hybrid striped bass (Morone chrysops x Morone saxatilis) following inoculation of the gills. Aquaculture 220, 165-173.
Meiri-Bendek, I., Lipkin, E., Friedmann, A., Leitner, G., Saran, A., Friedman, S., & Kashi, Y., 2002. A PCR-based method for the detection of Streptococcus agalactiae in milk. Journal of Dairy Science 85(7), 1717-1723.
Mian, G. F., Godoy, D. T., Leal, C. A. G., Yuhara, T. Y., Costa, G. M., & Figueiredo, H. C. P., 2009. Aspects of the natural history and virulence of S. agalactiae infection in Nile tilapia. Veterinary Microbiology 136(1-2), 180-183.
Nguyen, H.T., Kanai, K. & Yoshikoshi, K., 2001. Streptococcus iniae Infection in Japanese
Flounder Paralichthys olivaceus. Fish Pathology 36(1), 40-41.
Perera, R.P., Johnson, S.K. & Lewis, D.H., 1997. Epizootiological aspects of Streptococcus iniae affecting tilapia in Texas. Aquaculture 152, 25-33.
Robinson, J.A. & Meyer, F.P., 1996. Streptococcal Fish Pathogen. Journal of Bacteriology 92(2), 512.
Soto, E., Zayas, M., Tobar, J., Illanes, O., Yount, S., Francis, S., & Dennis, M. M., 2016. Laboratory-controlled Challenges of Nile Tilapia (Oreochromis niloticus) with Streptococcus agalactiae: Comparisons between Immersion, Oral, Intracoelomic and Intramuscular Routes of Infection. Journal of Comparative Pathology 155(4), 339- 345.
Suanyuk, N., Kong, F. R., Ko, D., Gilbert, G. L., & Supamattaya, K., 2008. Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia (Oreochromis sp.) and Nile tilapia (O. niloticus) in Thailand-Relationship to human isolates?. Aquaculture 284(1-4), 35-40.