Structure of the heart wall and existence of the blood cells in the heart of the dog-faced water snake Cerberus rynchops (Schneider, 1799)

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Lamai Thongboon
Sinlapachai Senarat
Jes Kettratad
Pisit Poolprasert
Sansareeya Wangkulangkul
Wannee Jiraungkoorskul
Ezra Mongkolchaichana
Theerakamol Pengsakul
Shuaiqin Huang
Chamnan Para

Abstract

A plurality of investigations on the heart structure of reptiles has been extensively reported; however, it has yet to conduct studies in Cerberus rynchops, the so-called common estuarine water-snake. In this current study, we histologically started to examine the heart wall of the atrium, ventricle chambers, and study the existence of the blood cells in the heart of sexually mature females. The selected samples were those C. rynchops from the Paknam Pranburi Estuary in Thailand using accurate histochemical techniques. The results showed that the heart structure of C. rynchops consisted of three primary compartments (sinus venosus, atrium, and ventricle). All compartments were surrounded by the heart wall, which was typically classified into three layers (the epicardium, the myocardium, and the endocardium), as likely seen in underlying patterns of reptilian heart. In particular, the myocardium of the ventricle in this snake was the thickest layer of the heart wall. Major components of the cardiac muscle fibers in the myocardium with green/ pinkness colors were associated with Massonʼs trichrome and Periodic Shift reagent methods, indicating to present various fibers and glycoproteins. Consequently, several blood cells were also detected in different areas. The normal and atretic red blood cells were seen in the cardiac fluid, as referred to the fluid inside the heart sac, whereas the mast cell was also observed in the heart wall especially myocardium.

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How to Cite
Thongboon, L., Senarat, S., Kettratad, J., Poolprasert, P., Wangkulangkul, S., Jiraungkoorskul, W., Mongkolchaichana, E., Pengsakul, T., Huang, S., & Para, C. (2018). Structure of the heart wall and existence of the blood cells in the heart of the dog-faced water snake Cerberus rynchops (Schneider, 1799). Veterinary Integrative Sciences, 16(2), 79–86. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/141530
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Research Articles

References

Alvarez-Pellitero, P., Palenzuela O., Sitjà-Bobadilla A. 2007. Histopathology and cellular response in Enteromyxum leei (Myxozoa) infections of Diplodus puntazzo (Teleostei). Parasitol. Int 57, 110-120.

Agius, C., Roberts, R.J. 2003. Melano-macrophage centres and their role in fish pathology. J. Fish Dis. 26, 499–509.

Burggren, W.W. 1987. Form and function in reptilian circulations. Am. Zool.27, 5–19.

Cerra, M.C., Canonaco, M., Acierno, R., Tota, B. 1997. Different binding activities of A- and B-type natriuretic hormones in the heart of two Antarctic teleosts, the red-blooded Trematomus bernacchii and the hemoglobinless Chionodraco hamatus. Comp. Biochem. Physiol. 118A, 993–999.

Dietrich, D., Krieger, H.O., 2009. Histological analysis of endocrine disruptive effects in small laboratory fish. Wiley-Interscience, Hoboken, N.J.
Farrell, A.P., Graperil, A.K., Frances, E.T.B. 1998. Comparative aspects of heart morphology. In: Gans, C., Gaunt, A.S. (Eds.), Biology of the Reptilia, Visceral organs. New York: Society for the Study of Amphibians and Reptiles. 19, 375–424.

Galli, S.J., Tsai M. 2008. Mast cells: Versatile regulators of inflammation, tissueremodeling, host defense and homeostasis. J. Dermatol. Sci.49, 7–19.

Genten, F., Terwinghe, E., Danguy, A., 2008. Atlas of fish histology. Science Publishers, Enfield, NH.

Icardo, J.M. 2012. The teleost heart: A morphological approach. In: Sedmera,D., Wang, T. (Eds.), Ontogeny and Phylogeny of the Vertebrate Heart Springer Science, Business Media, LLC, pp. 35–53.

Jensen, B., Moorman, A.F., Wang, T. 2014. Structure and function of the hearts of lizards and snakes. Biol. Rev. Camb. Philos. Soc. 89, 302–336.

Jensen, B., ven den Berg, G., ven den Doel, R., Wang, T. and Moorman, A.F.2013. Development of the hearts of lizards and snakes and perspective to cardiac evolution. Plos One. 8, e63651.

Metz, M., Grimbaldeston, M.A., Nakae, S., Piliponsky, A.M., Tsai, M., Galli,S.J. 2007. Mast cells in the promotion and limitation of chronic inflammation. Immunol. Rev. 217, 304–328

Murphy, J. 2010. Cerberus rynchops. The IUCN red list of threatened species. Version 2014. 2. www.iucnredlist.org, 18 September 2016.Olson, K.R. 2000. Circulation system. In: Ostrander, G.K. (ed.), The Laboratory Fish. 2nd ed, United Kingdom, Academic Press. pp. 369–378.

Presnell, M.J.K., Schreibman, P.M.P., 1997. Humason’s animal tissue techniques, 5th edition. ed. The Johns Hopkins University Press, Balti more.

Perni, S., Lyer, V.R. Franzini-Armstrong, C. 2012. Ultrastructure of cardiac muscle in reptiles and birds: optimizing and/or reducing the probability of transmission between calcium releases unites. J Muscle.Res. Cell. Motil. 33, 145-152.

Qayyum, M.A. 1972. Anatomy and histology of the specialized tissues of theheart of the domestic cat (Felis catus). Acta. Hictomica. 82, 352-367.

Roberts, R.J. (Ed.), 2012. Fish pathology, 4th ed. ed. Wiley-Blackwell, Chich ester, West Sussex ; Ames, Iowa.Senarat, S., Kettretad, J., Jiraungkoorskul, W. 2016. Histological evidence of the heart and ovarian vessels in the short mackerel, Rastrelliger brachysoma. EurAsian J. BioSci. 10, 13-21.

Sitja-Bobadilla, A. 2008. Fish immune response to myxozoan parasites. Parasite, 15, 420-425.

Snyder, P.S., Shaw, N.G., Heard, D.J. 1999. Two-dimensional echocardiographic anatomy of the snake heart (Python molurus bivittatus). Vet Radiol. Ultrasound. 40, 66-72.

Suvarna, S.K., Layton, C., Bancroft, J.D. (Eds.), 2013. Bancroft’s theory and practice of histological techniques, 7th ed.Churchill Livingstone,Oxford.

UNEP-WCMC. 2013. The Checklist of CITES Species Website. CITES Secretariat, Geneva, Switzerland. Compiled by UNEPWCMC, Cambridge, UK. https://checklist.cites.org, 18 September 2016

Webb, G.J.W., Heatwole, H., de Bavay, J. 1974. Comparative cardiac anatomy of the Reptilia. II. A critique of the literature on the Squamata and Rhynchocephalia. J. Morphol. 142, 1–20.

White, F.N. 1968. Functional anatomy of the heart of reptiles. Amer. Zool. 8,211–219.

Wyneken, J. 2009. Normal reptile heart morphology and function. Vet. Clin.North Am. Exo. Anim. Pract. 12, 51–63.