In vitro cytotoxicity test and antiviral activity of curcuminoids from turmeric extract against PRRS virus

Main Article Content

Pemika Anantikulchai
Pandhira Emprom
Kidsadagon Pringproa
Panuwat Yamsakul

Abstract

Porcine Reproductive and Respiratory Syndrome (PRRS) caused by PRRS virus is an important viral infectious disease of swine that causes immense economic loss in swine industries worldwide. Presently, the defensive and controls of PRRS outbreak are mostly depend on the farm biosecurity, gilt management and successive vaccination program. However, the disease control by those strategies remained uncertain and unpredictable. Therefore, seeking an alternative antiviral compound for preventing and controlling PRRS is of great interest. In the present study, we aim to investigate the anti-PRRSv property of the curcuma, compound derived from medicinal plant in Zingiberaceae family. Turmeric extraction (curcuminoids) was obtained and was increase dissolved by the solid dispersion technique and found that at the concentration of 0.00005 – 1.56 µg/mL showed no cytotoxicity effect on the MARC-145 cells. Interestingly, at the concentration of 1.56 µg/mL, curcuminoids showed significantly inhibit PRRS virus replication. This result is interesting in use herb extraction against PRRS infection and should be study, in vivo

Article Details

How to Cite
Anantikulchai, P., Emprom, P., Pringproa, K., & Yamsakul, P. (2017). In vitro cytotoxicity test and antiviral activity of curcuminoids from turmeric extract against PRRS virus. Veterinary Integrative Sciences, 15(3), 199–205. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/145707
Section
Research Articles

References

Chae, C. 2016. Porcine respiratory disease complex: Interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory
syndrome virus, and Mycoplasma hyopneumoniae. Vet. J. 212: 1-6.

Chen, D.Y., Shien, J.H., Tiley, L., Chiou, S.S., Wang, S.Y., Chang, T.J. 2010. Curcumin inhibits influenza virus infection and haemagglutination activity. Food. Chem. 119: 1346-1351.

Du, Y., Yoo, D., Paradis, M.A., Scherba, G. 2011. Antiviral Activity of Tilmicosin for Type 1 and Type 2 Porcine Reproductive And Respiratory Syndrome Virus In Cultured Porcine Alveolar Macrophages. J. Antivir. Antiretrovir. 3: 28-33.

Erik, D.C. 2004. Antiviral drug in current clinical use. J. Clin. Vir. 30: 115-133.

Ho, J.Y., Chang, H.W., Lin, C.F., Liu, C.J., Hsieh, C.F., Horng, J.T. 2014. Characterization of the AntiInfluenza Activity of the Chinese Herbal Plant Paeonia lactiflora. Viruses. 6: 1861-1875.

Ingkaninan, K., de Best, C.M., Irth, H., van der Heijden, R., Hofte, A.J.P., Karabatak, B. 2000. High performance liquid chromatography with on-line coupled UV-mass spectrophotometric-biochemical detection for identification of acetyl cholinesterase inhibitors from natural products. J. Chromatogr. A. 872: 61-73.

Ingkaninan, K., Hazekamp, A., Hoek, A.C., Balconi, S., Verpoorte, R. 2000. Application of a centrifugal partition chromatography in a general separation and dereplication procedure for plant extracts. J. Liq. Chrom. & Rel. Technol. 23: 2195-2208.

Labarque, G., Van Gucht, S., Van Reeth, K., Nauwynck, H., Pensaert, M. 2003. Respiratory tract protection upon challenge of pigs vaccinated with attenuated porcine reproductive and respiratory syndrome virus vaccines. Vet. Microbiol. 95: 187-197.

Lambert, M.È., Poljak. Z., Arsenault. J., D’Allaire, S. 2012. Epidemiological investigations in regard to porcine reproductive and respiratory syndrome (PRRS) in Quebec, Canada. Part 1: Biosecurity practices and their geographical distribution in two areas of different swine density. Prev. Vet. Med. 104: 74-83.

Pringproa, K., Khonghiran, O., Kunanoppadol, S., Potha, T., Chuammitri, P. 2014. In vitro Virucidal and Virustatic Properties of the Crude Extract of Cynodon dactylon Against Porcine Reproductive and Respiratory Syndrome Virus. Vet. Med. Inter. ID. 947589.

Olanratmanee, E.O., Wongyanin, P., Thanawongnuwech, R., Tummaruk, P. 2015. Prevalence of porcine reproductive and respiratory syndrome virus detection in aborted fetuses, mummified fetuses and stillborn piglets using quantitative polymerase chain reaction. J. Vet. Med. Sci. 77: 1071-1077.

Ouyang, K., Hiremath, J., Binjawadagi, B., Shyu, D.L., Dhakal, S., Arcos, J. 2016. Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSv) vaccine in a heterologous virus challenge study. Vet. Res. 47: 1-11.

Oyuntsetseg, N., Khasnatinov, M.A., Molor-Erdene, P., Oyunbileg, J., Liapunov, A.V., Danchinova, G.A. 2014. Evaluation of direct antiviral activity of the Deva-5 herb formulation and extracts of five Asian plants against influenza A virus H3N8. BMC. Complement. Altern. Med. 14: 1-9.

Sornpet, B., Potha, T., Tragoolpua, Y., Pringproa K. 2017. Antiviral activity of five Asian medicinal pant crude extracts against highly pathogenic H5N1 avian influenza virus. Asian Pac J Trop Med. 10: 871– 876.

Tian, K., Yu, X., Zhao, T., Feng, Y., Cao, Z., Wang, C. 2007. Emergence of Fatal PRRSv Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark. PLoS. ONE. 2: e526.

Wang, Y., Pan, M., Cheng, A., Lin, L., Ho, Y., Hsieh, C. 1997. Stability of curcumin in buffer solutions and characterization of its degradation products. J. Pharm. Biomed. Anal. 12: 1867-76.

Zandi, K., Ramedani, E., Mohammadi, K., Tajbakhsh, S., Deilami, I., Rastian, Z. 2010. Evaluation of antiviral activities of curcumin derivatives against HSV-1 in Vero cell line. Nat. Prod .Commun. 5: 1935-1938.

Zhang, Y.J., Stein, D.A., Fan, S.M., Wang, K.Y., Kroeker, A.D., Meng, X.J., Iversen, P.L., Matson, D.O. 2006. Suppression of porcine reproductive and respiratory syndrome virus replication by morpholino antisene oligomers. Vet. Micro. 117: 117-129.

Zorofchian, M.S., Abdul, K.H., Hassandarvish, P., Tajik, H., Abubakar, S., Zandi, K. 2014. A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin. BioMed. Res. Inter. ID. 186864.