Association analysis of candidate gene polymorphisms with egg production in Japanese quails (Coturnix japonica)
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Abstract
This study was conducted to identify genotypes and evaluate the effect of polymorphisms in growth hormone (GH), prolactin (PRL), bone morphogenetic protein receptor-Type IB (BMPR-1B) and melatonin receptor-type 1C (MTNR-1C) genes on egg production of Japanese quail during 20 weeks of egg laying. PCR-RFLP and PCR-SSCP methods were used to determine the genotype of all genes. Our results showed that the genotypes at loci C2161G in PRL and G294A in MTNR-1C were monomorphic as C and A, respectively. For the A237B in GH and A290T BMPR-1B mutations, two alleles were detected, giving three genotypes at each locus. In addition, the 24-bp difference of two genotype patterns provided an Indel-358 mutation in PRL while three alleles were identified at locus A459TC (MTNR-1C). Taken together, the polymorphic sites did not affect egg production except the A459TC locus in MTNR-1C gene (P=0.002), where quails carrying AA and CC genotypes provided the highest egg number (115.6-116.6 eggs/quail). Thus, the A459TC single nucleotide polymorphism is suggested to apply in other populations and it should be considered as a candidate marker for egg production.
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