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The present study was to evaluate the inhibitory capability of two different herbal tea formulas (i) formula I (T1); 40% oolong 30% jiaogulan and 30% dear’s horn fruit and (ii) formula II (T2); 90% green tea, 4% mulberry leaf, 3% pandanus, 3% jiaogulan on enzymes associated with hyperglycemia and hyperlipidemia as well as the lipid profile values in rat model. The effects of two tea formulas on α-amylase, α-glucosidase, pancreatic lipase and lipoprotein lipase was conducted using colorimetric enzyme activity assay kits. In addition, Wistar rats intraperitoneally injected with 75% egg yolk were orally administrated with the two formulas (T1&T2). Our results showed that T1 had better α-amylase and α-glucosidase inhibitory properties, whereas T2 had better pancreatic lipase inhibitory properties. Infusion time had trivial effect on the inhibitory properties of both formulas. Nevertheless, both herbal mixed tea formulas could not inhibit lipoprotein lipase activity. In vivo study in hyperlipidemia-induced male wistar rat model revealed that T2 had a trend of improving hyperlipidemia rat lipid profile by lowering TC, TG and LDL-C contents along with increasing HDL-C level in blood plasma when compared with the control but not for T1. The in vitro and in vivo findings of this study demonstrated that T2 may serve as an inhibitory agent against enzymes involved with hyperglycemia and hyperlipidemia.
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