Hypoglycemic and hypolipidemic properties of herbal tea on Wistar rat
Article Sidebar
Main Article Content
Abstract
The present study was to evaluate the inhibitory capability of two different herbal tea formulas (i) formula I (T1); 40% oolong 30% jiaogulan and 30% dear’s horn fruit and (ii) formula II (T2); 90% green tea, 4% mulberry leaf, 3% pandanus, 3% jiaogulan on enzymes associated with hyperglycemia and hyperlipidemia as well as the lipid profile values in rat model. The effects of two tea formulas on α-amylase, α-glucosidase, pancreatic lipase and lipoprotein lipase was conducted using colorimetric enzyme activity assay kits. In addition, Wistar rats intraperitoneally injected with 75% egg yolk were orally administrated with the two formulas (T1&T2). Our results showed that T1 had better α-amylase and α-glucosidase inhibitory properties, whereas T2 had better pancreatic lipase inhibitory properties. Infusion time had trivial effect on the inhibitory properties of both formulas. Nevertheless, both herbal mixed tea formulas could not inhibit lipoprotein lipase activity. In vivo study in hyperlipidemia-induced male wistar rat model revealed that T2 had a trend of improving hyperlipidemia rat lipid profile by lowering TC, TG and LDL-C contents along with increasing HDL-C level in blood plasma when compared with the control but not for T1. The in vitro and in vivo findings of this study demonstrated that T2 may serve as an inhibitory agent against enzymes involved with hyperglycemia and hyperlipidemia.
Article Details
Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.
References
Azlan, W.M.B.W.M. 2010. Extracts of the Aerial Roots from Pandanus amaryllifolius. Doctoral dissertation, Universiti Teknologi Mara. Bandana, D., Neha, S., Dinesh, K., Kama, J. 2013. Morus alba Linn: A phytophamacological review. Int. J. Pharm. Pharm. 5(2): 14-18.
Cabrera, C., Artacho, R., Gimenez, R. 2006. Beneficial effects of green tea-a review. J. Am. Coll. Nutr. 25(2): 79-99.
Chiabchalard, A., Nooron, N. 2015. Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract. Pharmacogn Mag. 11(41): 117–122.
Crespy, V., Williamson, G. 2004. A Review of the health effects of green tea catechins in in vivo animal models. J. Nutr. 134(12): 3431-3440.
Eichhorn, P., Schwandt, P., Richter, W.O. 1994. Proopiomelanocorticotropin (POMC) peptides lipoprotein lipase activity in vitro. Pergamon. 16(4): 665-671.
Guerciolini, R. 1997. Mode of action of orlistat. Int. J. Obes. Relat. Metab. Disord. 21: 12-23.
Hsu, Y.W., Tsai, C.F., Chen, W.K., Huang, C.F., Yen, C.C. 2011. A subacute toxicity evaluation of green tea (Camellia sinensis) extract in mice. J. Food Chem Toxical. 49(10): 2624-2630.
Imai, K., Nakachi, K. 1995. Cross sectional study of effects of drinking green tea on cardiovascular and liver diseases. British Med. J. 310(6981): 693-696.
Kaewpiboon, C., Lirdprapamongkol, K., Srisomsap, C., Winayanuwattikun, P., Yongvanich, T., Puwaprisirisan, P., Svasti, J., Assavalapsakul, W. 2012. Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants. BMC Complement. Altern. Med. 12(1): 217-226.
Kumar, G., Sharmila Banu, G., Murugesan, A.G., Rajasekara Pandian, M. 2007. Preliminary Toxicity and Phytochemical Studies of Aqueous Bark Extract of Helixteres isora L. Int. J. Pharm. 3(1): 96-100.
Kyle, A.M. J., Morrice, P. C., McNeill, G., Duthie G. G. 2007. Effects of Infusion Time and Addition of Milk on Content and Absorption of Polyphenols from Black Tea. J. Agric. Food Chem. 55: 48894894.
Megally, S., Aktan, F., Davies, N.M., Roufogalis, B.D. 2005. Phytopreventative anti-hyperlipidemic effects of Gynostemma Pentaphyllum in rats. J. Pharm. Sci. 8(3): 507-515.
Mishra, R.N., Dharnidhar, J. 2011. Jiao Gu Lan (Gynostemma pentaphyllum): The Chinese Rasayan-current research scenario. Int. J. Res. Pharm. Biomed. Sci. 2(4): 1483-1502.
Nakai, M., Fukui, Y., Asami, S., Toyoda-ono, Y., Iwashita, T., Shibata, H., Mitsunaga, T., Hashimoto, F., Kiso, Y., 2005. Inhibitory effects of Oolong tea polyphenols on pancreatic lipase in vitro. J. Agric. Food. Chem. 53(11): 4593-4598.
Nguyen, T.T.H., Jung, S.H., Lee, S., Ryu, H.J., Kang, H.K., Moon, Y.H., Kim, Y.M. 2012. Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition. Biotech. Bioprocess. Eng. 17(5): 966-971.
Raja, A.B., Elanchezhiyan, C., Sethupathy, S. 2010. Antihyperlipidemic activity of Helicteres isora fruit extract on streptozotocin induced diabetic male Wistar rats. Eur Rev. Med. Pharmacol. Sci. 14(3): 191-196.
Rohn, S., Rawel, H.M., Kroll, J. 2002. Inhibitory effects of plant phenols on the activity of selected enzymes. J. Agric. Food. Chem. 50(12): 3566-3571.
Sharangi, A.B. 2009. Medicinal and therapeutic potentialities of tea (Camellia sinensis L.) – A review. Food Res. Int. 42(5): 529-535.
Shepard, T.Y., Jensen, D.R., Blotner, S., Zhi, J., Guerciolini, R., Pace D., Eckel, R.H. 2002. Orlistat fails to alter postprandial plasma lipid excursions or plasma lipases in normal-weight male volunteers. Int. J. Obesity. 24(2): 187-194.
Shi, Q.Y., Schlegel, V. 2012. Green tea as an agricultural based health promoting food: the past five to ten years. J. Agric. Food. Chem. 2(4): 393-413.
Song, L., Dong, L., Bo, H., Yuxin, C., Xiaocong, L., Youwei, W. 2013. Inhibition of pancreatic lipase, αglucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves. J. Ethnopharmacol. 149(1): 263– 269.
Stancu, C., Sima, A. 2001. Statins: mechanism of action and effects. J. Mol. Cell. Med. 5(4): 378-387.
Sumbul, S., Ahmed, S.I. 2012. Anti-hyperlipidemic activity of Carissa carandas (Auct.) leaves extract in egg yolk induced hyperlipidemic rats. J. Basic Appl. Sci. 8: 40-50.
Tadera, K., Minami, Y., Takamatsu, K. Matsuoka, T. 2006. Inhibition of α-glucosidase and α-amylase by flavonoids. J. Nat. Sci. Vitaminol. 52(2): 149-153.
Thilagam, E., Parimaladevi, B., Kumarappan, C., Mandal, S.C. 2013. α-Glucosidase and α-amylase inhibitory activity of Senna surattensis. J. Acupunct. Meridian. Stud. 6: 24-30.
Tsubono, Y., Tsugane, S. 1997. Green tea intake in relation to serum lipid levels in middle-aged Japanese men and women. Ann. Epidemiol. 7(4): 280-284.
Venkatesh, S., Reddy, G.D., Reddy, Y.S.R., Sathyavathy, D., Madhava, R.B. 2004. Effect of Helicteres isora root extracts on glucose tolerance in glucoseinduced hyperglycemic rats. Fitoterapia. 75(3): 364-367.
Yadav, A.V., Kawale, L.A., Nade, V.S. 2008. Effect of Morus alba L. (mulberry) leaves on anxiety in mice. Indian J. Phamacol. 40(1): 32-36.
Zhao, H.L., Sim, J.S., Shim, S.H., Ha, Y.W., Kang, S.S., Kim, Y.S. 2005. Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction. Int. J. Obesity 29(8): 983990.