Study of bacterial species and antimicrobial sensitivity in canine oral cavity at small animal teaching hospital, Chiang Mai University
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Abstract
The aims of this study were to determine bacteria species in oral cavity of canine and their antimicrobial susceptibility. Oral swabs were taken from 20 dogs at the Small Animal Teaching Hospital, Chiang Mai University. Bacterial identification and antimicrobial susceptibility test were revealed. The results presented 16 bacterial species in this study. The predominant grampositive bacteria were Staphylococcus aureus (6.25%), Corynebacterium spp. (6.25%) and Staphylococcus epidermidis (3.21%), respectively. The predominant gram-negative bacteria were Actinobacillus actinomycetemcomitans (17.19%) and Pseudomonas spp. (12.5%), respectively. Moreover, 12.59% of gram-negative rods and 6.25% of gram-negative cocci were species unidentified. This present study showed that gingivitis group was variously bacterial species than normal group. Furthermore, the antimicrobial sensitivity tests of gram-negative bacteria were susceptible to sulfamethoxazole and trimethoprim (62.5%), amoxicillin / clavulanic acid (37.5%), penicillin (50%). Moreover, the gram-negative bacteria were susceptible to cefazolin, enrofloxacin and gentamicin but 81.2% were resistant to penicillin.
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References
Albuquerque, C., Morinha, F., Requicha, J., Martins, T., Dias, I., Guedes-Pinto, H., Bastos E., Viegas, C., 2012. Canine periodontitis: the dog as an important model for periodontal studies. Vet J. 191: 299-305.
Baquero, F., 1997. Gram-positive resistance: challenge for the development of new antibiotics. J Antimicrob Chemother. 39: 1-6.
Daniluk, T., Tokajuk, G., Cylwik-Rokicka, D., Rozkiewicz, D., Zaremba, M.L., Stokowska, W., 2005. Aerobic and anaerobic bacteria in subgingival and supragingival plaques of adult patients with periodontal disease. Adv Med Sci. 51: 81-85.
Dogan, B., Antinheimo, J., Cetiner, D., Bodur, A., Emingil, G., Buduneli, E., Uygur, C., Firatli, E., Lakio, L., Asikainen, S., 2003. Subgingival microflora in Turkish patients with periodontitis. J Periodontol. 74: 803-814.
Goldstein, E.J., Citron, D.M., Wield, B., Blachman, U., Sutter, V.L., Miller, T.A., Finegold, S.M., 1978. Bacteriology of human and animal bite wounds. J Clin Microbiol. 8: 667-672.
Grayson, M.L., Thauvin-Eliopoulos, C., Eliopoulos, G.M., Yao, J.D., DeAngelis, D.V., Walton, L. , Woolley, J.L., Moellering, Jr R.C., 1990. Failure of trimethoprim-sulfamethoxazole therapy in experimental enterococcal endocarditis. Antimicrob Agents Chemother. 34: 1792-1794.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T., 1994. Bergey’s manual of determinative bacteriology. 9th edn. Baltimore: Williams and Wilkins, 75- 121.
Imbronito, A.V., Okuda, O.S., Maria de Freitas, N., Moreira Lotufo, R.F., Nunes, F.D., 2008. Detection of herpesviruses and periodontal pathogens in subgingival plaque of patients with chronic periodontitis, generalized aggressive periodontitis, or gingivitis. J Periodontol. 79: 2313-2321.
Lalitha, M.K., 2004. Manual on antimicrobial susceptibility testing. Performance standards for antimicrobial testing: Twelfth Informational Supplement, 56238: 454-456.
Luvira, V., 2006. Overveiw of antibiotic resistance. Songklanagarind Medical Journal. 24: 453-459.
Meyers, B., Schoeman, J.P., Goddard, A., Picard, J., 2008. The bacteriology and antimicrobial susceptibility of infected and non-infected dog bite wounds: fifty cases. Vet microbial. 127: 360-368.
Moellering, R.C. Jr, 1998. Problems with antimicrobial resistance in gram-positive cocci. Clin Infect Dis. 26: 1177-1178.
Offenbacher, S., 1996. Periodontal diseases: pathogenesis. Ann Periodontol. 1: 821-878.
Pedersen, K., Pedersen, K., Jensen, H., Finster, K., Jensen, V.F., Heuer, O.E., 2007. Occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs. J Antimicrob Chemother. 60: 775-781.
Prescott, J.F., Yielding, K.M., 1990. In vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin. Can J Vet Res. 54: 195. Rice, L.B., 2006. Antimicrobial resistance in gram-positive bacteria. Am J Infect Control. 34: S11-S19.
Schroeder, C.M., Meng, J., Zhao, S., DebRoy, C., Torcolini, J., Zhao, C. McDermott, P.F., Wagner, D.D., Walker, R.D., White, D.G., 2002. Antimicrobial resistance of Escherichia coli O26, O103, O111,
O128 and O145 from animals and humans. Emerg Infect Dis. 8: 1409-1414.
Soares, G.M.S., Figueiredo, L.C., Faveri, M., Cortelli, S.C., Duarte, P.M., Feres, M., 2012. Mechanisms of action of systemic antibiotics used in periodontal treatment and mechanisms of bacterial resistance to these drugs. J Appl Oral Sci. 20: 295-304.
Talan, D.A., Staatz, D., Staatz, A., Goldstein, E.J., Singer, K., Overturf, G.D., 1989. Staphylococcus intermedius in canine gingiva and canineinflicted human wound infections: laboratory characterization of a newly recognized zoonotic pathogen. J Clin Microbiol. 27: 78-81.
Zambori, C., Tirziu, E., Nichita, I., Cumpanasoiu, C., Gros, R.V., Seres, M., Mladin, B., Mot, D., 2012. Biofilm implication in oral diseases of dogs and cats. Scientific Papers Animal Science and Biotechnologies. 45: 208-212.