Molecular characterization of S1 gene of nephropathogenic infectious bronchitis virus isolated from disease outbreaks in Chiang Mai province, Thailand
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Published:
Aug 28, 2015
Keywords:
infectious bronchitis virus S1 gene phylogenetic analysis
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Abstract
The purpose of this study was to determine the molecular characteristic in S1 gene of nephropathogenic infectious bronchitis virus (IBV) isolated from disease outbreaks in Chiang Mai, Thailand. Five field isolates of IBV were collected from chicken flocks which were suffering from respiratory and nephropathological symptoms and lesions in Chiang Mai province during 2010-2011. After propagation of the viruses in embryonated chicken eggs, 988 base pairs of the S1 gene covering a hypervariable region was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and the purified RT-PCR products were sequenced. The homology and phylogenetic analysis showed that the isolates were grouped into group II Thai IBV, QX-like IBV. S1 gene of the isolates showed nucleotide identity of 99.4100% with each other and 97.3-97.5% with original QXIBV. Analysis of the deduced amino acid sequences revealed that the isolates had 98.8-100% identity with each other and 95-96.9% with original QXIBV. These findings indicated that nephropathogenic IBVs isolated from field outbreaks in Chiang Mai, Thailand during 2010-2011 are the QX-like IBV and they have a minor change in their S1 gene.
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Pohuang, T., Junnu, S., & Chotinun, S. (2015). Molecular characterization of S1 gene of nephropathogenic infectious bronchitis virus isolated from disease outbreaks in Chiang Mai province, Thailand. Veterinary Integrative Sciences, 13(2), 81–91. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/146327
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Research Articles
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References
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Pohuang, T., Chansiripornchai, N., Tawatsin, A. and Sasipreeyajan, J. (2009a). Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. Journal of Veterinary Science, 10, 219-223.
Pohuang, T., Chansiripornchai, N., Tawatsin, A. and Sasipreeyajan, J. (2009b). Pathogenesis of a new genotype infectious bronchitis virus isolated in chickens. The Indian Veterinary Journal, 86, 1110-1112.
Pohuang, T., Chansiripornchai, N., Tawatsin, A. and Sasipreeyajan, J. (2011). Sequence analysis of S1 genes of infectious bronchitis virus isolated in Thailand during 2008-2009: Identification of natural recombination in the field isolates. Virus Genes, 43(2), 254-260.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28(10), 2731-2739.
Tolskaya, E.A., Romanova, L.A., Blinow, V.M., Virtorova, E.G., Sinyakov, A.N., Kolesnikova, M.S. and Agol, V.I. (1987). Studies on the recombination between RNA genomes of poliovirus: the primary structure and nonrandom distribution of crossover regions in the genomes of intertypic poliovirus recombinants. Virology, 161, 54-61.
Wang, C.H. and Huang, Y.C. (2000). Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus. Archive Virology, 145, 291-300.
Ziegler, A.F., Ladman, B.S., Dunn, P.A., Schneider, A., Davison, S., Miller, P.G., Lu, H., Weinstock, D., Slem, M., Eckroade, R.J. and Gelb, J., Jr. (2002). Nephropathogenic infectious bronchitis in Pennsylvania chickens 1997-2000, Avian Diseases, 46,847-858.
Animas, S.B., Otsuki, K., Hanayama, M., Sanekata, T. and Tsubokura, M. (1994). Experimental infection with avian infectious bronchitis virus (Kagoshima-34 strain) in chicks at diferrent ages. Journal of Veterinary Medical Science, 56(3), 443447.
Beato, M.S., De Battisti, C., Terregino, C., Drago, A., Capua, I. and Ortali, G. (2005). Evidence of circulation of Chinese strain of infectious bronchitis virus (QXIBV) in Italy. Veterinary Record, 156, 720.
Benyeda, Z., Mato, T., Suveges, T., Szabo, E., Kardi, V., Abonyi-Toth, Z., Rusvai, M. and Palya, V. (2009). Comparison of the pathogenecity of QX-like, M41 and 793/B infectious bronchitis strains from different pathological conditions. Avian Pathology, 38, 449-456.
Bochkov, Y.A., Batchenko, G.V., Shcherbakova, L.O., Borisov, A.V. and Drygin, V.V. (2006). Molecular epizootiology of avian infectious bronchitis in Russia. Avian Pathology, 35, 379-393.
Cavanagh, D., Davis, P.J. and Cook, J.K.A. (1992). Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathology, 21, 401-408.
Cavanagh, D. and Naqi, S.A. (2003). Infectious bronchitis. Diseases of poultry 11th edition. Iowa, Iowa State Press. Dhinakar Raj, G. and Jones, R.C. (1997). Infectious bronchitis virus: immunopathogenesis of infection in the chicken. Avian Pathology, 26, 677-706.
Gelb, J., Jr., Wolff, J.B. and Moran, C.A. (1991). Variant serotypes of infectious bronchitis virus isolated from commercial layer and broiler chickens. Avian Diseases, 35(1), 82-87.
Gough, R.E., Randall, C.J., Dagless, M., Alexander, D.J., Cox, W.J. and Pearson, D. (1992). A ‘new’ strain of infectious bronchitis virus infecting domestic fowl in Great Britain. Veterinary Record, 130, 493-494.
Gough, R.E., Cox, W.J., Welchman, D., Worthington, K.J., Jones, R.C. (2008). Chinese QX strain of infectious bronchitis virus isolated in UK. Veterinary Record, 162, 99-100.
Hall, T.A. (1999). BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Huang, Y.P., Lee, H.C., Cheng, M.C. and Wang, C.H. (2004). S1 and N gene analysis of avian infectious bronchitis viruses in Taiwan. Avian Diseases, 48(3), 581-589.
Jia, W., Karaca, K., Parrish, C.R. and Naqi, S.A. (1995). A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains. Archive Virology, 140, 259-271.
Kant, A., Koch, G., Roozelaar, D.J., Kusters, J.G., Poelwijk, F.A.J. and van der Zeijst, B.A.M. (1992). Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopeptide. Journal of General Virology, 73, 591-596.
Keeler, C.L., Jr., Reed, K.L., Nix, W.A. and Gelb, J., Jr. (1998). Serotype identification of avian infectious bronchitis virus by RTPCR of the peplomer (S1) gene. Avian Diseases, 42, 275-284.
Kingham, B.F., Keeler, C.L., Jr, Nix, W.A., Ladman, B.S. and Gelb, J., Jr. (2000). Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S1 gene. Avian Diseases, 44, 325-335.
Kusters, G.J., Niesters, M.G.H., Lenstra, A.J., Horzinek, C.M. and van der Zeijst, M.A.B. (1989). Phylogeny of antigenic variants of avian coronavirus IBV. Virology, 169, 217-221.
Kwon, H.M., Jackwood, M.W., Brown, T.P. and Hilt, D.A. (1993). Polymerase chain reaction and a biotin-labeled DNA probe for detection of infectious bronchitis virus in chickens. Avian Diseases, 37, 149-156.
Lai, M.M.C. and Holmes, K.V. (2001). Coronaviridae: the viruses and their replication. Fields virology. 4th ed. Philadelphia, PA: Lippincott Williams and Wilkins.
Lee, C.W., Hilt, D.A. and Jackwood, M.W. (2001). Identification and analysis of the Georgia 98 serotype, a new serotype of infectious bronchitis virus. Avian Diseases, 45, 164-172.
Liu, S. and Kong, X. (2004). A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China. Avian Pathology, 33, 321-327.
Pohuang, T., Chansiripornchai, N., Tawatsin, A. and Sasipreeyajan, J. (2009a). Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. Journal of Veterinary Science, 10, 219-223.
Pohuang, T., Chansiripornchai, N., Tawatsin, A. and Sasipreeyajan, J. (2009b). Pathogenesis of a new genotype infectious bronchitis virus isolated in chickens. The Indian Veterinary Journal, 86, 1110-1112.
Pohuang, T., Chansiripornchai, N., Tawatsin, A. and Sasipreeyajan, J. (2011). Sequence analysis of S1 genes of infectious bronchitis virus isolated in Thailand during 2008-2009: Identification of natural recombination in the field isolates. Virus Genes, 43(2), 254-260.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28(10), 2731-2739.
Tolskaya, E.A., Romanova, L.A., Blinow, V.M., Virtorova, E.G., Sinyakov, A.N., Kolesnikova, M.S. and Agol, V.I. (1987). Studies on the recombination between RNA genomes of poliovirus: the primary structure and nonrandom distribution of crossover regions in the genomes of intertypic poliovirus recombinants. Virology, 161, 54-61.
Wang, C.H. and Huang, Y.C. (2000). Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus. Archive Virology, 145, 291-300.
Ziegler, A.F., Ladman, B.S., Dunn, P.A., Schneider, A., Davison, S., Miller, P.G., Lu, H., Weinstock, D., Slem, M., Eckroade, R.J. and Gelb, J., Jr. (2002). Nephropathogenic infectious bronchitis in Pennsylvania chickens 1997-2000, Avian Diseases, 46,847-858.