Effects of quercetin on chicken heterophil cellular functions
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Published:
Dec 30, 2014
Keywords:
chicken heterophil quercetin cellular functions
Main Article Content
Abstract
Chicken heterophils responds to microbial invasions by utilizing innately equipped-intracellular and extracellular killing mechanisms. The objective of this research was to study the effects of quercetin (QH) on chicken heterophil effector functions, namely; ROS generation, phagocytosis & bacterial killing, and Heterophil Extracellular Traps (HETs). To conduct the experiments, BM-derived heterophils were fi rst primed with 50 μM QH for 30 min and subsequently stimulated with PMA. The results showed that ROS molecules were remarkably increased in QH-primed group (p=0.005) whereas quercetin slightly suppressed phagocytosis of fl uorescent labeling of Salmonella Enteritidis (SE) (p=0.62). Supplementation of quercetin aided heterophils in killing of live SE more effi ciently than PBS (p=0.04). Extracellular killing was assessed by enumeration of HETs. The observation of HET structures obviously revealed that the QH-primed cells had note worthy HET-DNA release (p=0.007). Taken together, 50 μM of quercetin supplementation enhances functional activities of heterophils in the killing of bacteria
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How to Cite
Nojit, P., Ampaipun, J., & Chuammitri, P. (2014). Effects of quercetin on chicken heterophil cellular functions. Veterinary Integrative Sciences, 12(3), 167–178. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/146488
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Research Articles
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References
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Souto, F. O., Zarpelon, A. C., Staurengo-Ferrari, L., Fattori, V., Casagrande, R., Fonseca, M. J. (2011). Quercetin reduces neutrophil recruitment induced by CXCL8, LTB4, and fMLP: inhibition of actin polymerization. Journal of Natural Products, 74(2), 113-118.
Suri, S., Taylor, M. A., Verity, A., Tribolo, S., Needs, P. W., Kroon, P. A. (2008). A comparative study of the effects of quercetin and its glucuronide and sulfate metabolites on human neutrophil function in vitro. Biochemical Pharmacology, 76(5), 645-653.
Temrangsee, P., Kondo, S., & Itharat, A. (2011). Antibacterial activity of extracts from fi ve medicinal plants and their formula against bacteria that cause chronic wound infection. Journal of the Medical Association of Thailand, 94(12), S166-171.
Wells, L. L., Lowry, V. K., Deloach, J. R., & Kogut, M. H. (1998). Age-dependent phagocytosis and bactericidal activities of the chicken heterophil. Developmental & Comparative Immunology, 22(1), 103-109.
Arima, H., Ashida, H., & Danno, G.-i. (2002). Rutin-enhanced antibacterial activities of fl avonoids against Bacillus cereus and Salmonella enteritidis. Bioscience, Biotechnology, and Biochemistry, 66(5), 1009-1014.
Boots, A. W., Haenen, G. R., & Bast, A. (2008). Health effects of quercetin: from antioxidant to nutraceutical. European Journal of Pharmacology, 585(2), 325-337.
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S. (2004). Neutrophil extracellular traps kill bacteria. Science, 303 (5663), 1532-1535.
Brooks, R. L., Bounous, D. I., & Andreasen, C. B. (1996). Functional comparison of avian heterophils with human and canine neutrophils. Comparative Haematology International, 6(3), 153-159.
Chan, S.-T., Chuang, C.-H., Yeh, C.-L., Liao, J.-W., Liu, K.-L., Tseng, M.-J. (2012). Quercetin supplementation suppresses the secretion of pro-infl ammatory cytokines in the lungs of Mongolian gerbils and in A549 cells exposed to benzo [a] pyrene alone or in combination with β-carotene: in vivo and ex vivo studies. The Journal of Nutritional Biochemistry, 23(2), 179-185.
Chuammitri, P., Ostojic, J., Andreasen, C. B., Redmond, S. B., Lamont, S. J., & Palic, D. (2009). Chicken heterophil extracellular traps (HETs): Novel defense mechanism of chicken heterophils. Veterinary Immunology and Immunopathology, 129(1-2), 126-131.
Chuammitri, P., Redmond, S. B., Kimura, K., Andreasen, C. B., Lamont, S. J., & Palić, D. (2011). Heterophil functional responses to dietary immunomodulators vary in genetically distinct chicken lines. Veterinary Immunology and Immunopathology, 142(3), 219-227.
Cushnie, T., & Lamb, A. J. (2005). Antimicrobial activity of fl avonoids. International Journal of Antimicrobial Agents, 26(5), 343-356.
Das, T., Mukherjee, S., & Chaudhuri, K. (2012). Effect of quercetin on Vibrio cholerae induced nuclear factor-B activation and interleukin-8 expression in intestinal epithelial cells. Microbes and Infection, 14(9), 690-695.
Ermert, D., Urban, C. F., Laube, B., Goosmann, C., Zychlinsky, A., & Brinkmann, V. (2009). Mouse neutrophil extracellular traps in microbial infections. Journal of Innate Immunity, 1(3), 181-193.
Genovese, K. J., He, H., Swaggerty, C. L., & Kogut, M. H. (2013). The avian heterophil. Developmental & Comparative Immunology, 41(3), 334-340.
He, H., Farnell, M. B., & Kogut, M. H. (2003). Infl ammatory agonist stimulation and signal pathway of oxidative burst in neonatal chicken heterophils. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 135(1), 177-184.
He, H., Genovese, K. J., Swaggerty, C. L., Nisbet, D. J., & Kogut, M. H. (2008). Differential induction of nitric oxide, degranulation, and oxidative burst activities in response to microbial agonist stimulations in monocytes and heterophils from young commercial turkeys. Veterinary Immunology and Immunopathology, 123(3–4), 177-185.
Kim, H. P., Son, K. H., Chang, H. W., & Kang, S. S. (2004). Anti-infl ammatory plant fl avonoids and cellular action mechanisms. Journal of Pharmacological Sciences, 96(3), 229-245. Lambeth, J. D. (2004). NOX enzymes and the biology of reactive oxygen. Nature Reviews Immunology, 4(3), 181-189.
Liu, J., Li, X., Yue, Y., Li, J., He, T., & He, Y. (2005). The inhibitory effect of quercetin on IL-6 production by LPS stimulated neutrophils. Cellular & Molecular Immunology, 2(6), 455-460.
Liu, Y., Li, Y., Liu, H., Suo, Y., Hu, L., Feng, X. (2013). Effect of quercetin on performance and egg quality during the late laying period of hens. British Poultry Science, 54(4), 510-514.
Piccoli, C., D’Aprile, A., Ripoli, M., Scrima, R., Lecce, L., Boffoli, D. (2007). Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species. Biochemical and Biophysical Research Communications, 353(4), 965-972.
Röhm, M., Grimm, M. J., D'Auria, A. C., Almyroudis, N. G., Segal, B. H., & Urban, C. F. (2014). NADPH oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis. Infection and Immunity, 82(5), 1766-1777.
Rupasinghe, H. P. V., Ronalds, C. M., Rathgeber, B., & Robinson, R. A. (2010). Absorption and tissue distribution of dietary quercetin and quercetin glycosides of apple skin in broiler chickens. Journal of the Science of Food and Agriculture, 90(7), 1172-1178.
Souto, F. O., Zarpelon, A. C., Staurengo-Ferrari, L., Fattori, V., Casagrande, R., Fonseca, M. J. (2011). Quercetin reduces neutrophil recruitment induced by CXCL8, LTB4, and fMLP: inhibition of actin polymerization. Journal of Natural Products, 74(2), 113-118.
Suri, S., Taylor, M. A., Verity, A., Tribolo, S., Needs, P. W., Kroon, P. A. (2008). A comparative study of the effects of quercetin and its glucuronide and sulfate metabolites on human neutrophil function in vitro. Biochemical Pharmacology, 76(5), 645-653.
Temrangsee, P., Kondo, S., & Itharat, A. (2011). Antibacterial activity of extracts from fi ve medicinal plants and their formula against bacteria that cause chronic wound infection. Journal of the Medical Association of Thailand, 94(12), S166-171.
Wells, L. L., Lowry, V. K., Deloach, J. R., & Kogut, M. H. (1998). Age-dependent phagocytosis and bactericidal activities of the chicken heterophil. Developmental & Comparative Immunology, 22(1), 103-109.