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AbstractThis study wasaimed tocomparea discriminatory powerand to differentiateSalmonella Rissen and Salmonella Typhimurium DNA fingerprint by 2 molecular genotyping methods: Pulsed-Field Gel Electrophoresis (PFGE) and Repetitive Element Sequence-based PCR (rep-PCR). Salmonellaisolates from pig farms in Chiang Mai and Lampoon province, including 14 Salmonella Rissen and 16 Salmonella Typhimurium isolates samples were included in the study. Salmonella serotypes were previously confirmed by National Salmonellae and Shigellae Center, Department of Medical Sciences, Ministry of Public Health, Thailand. All samples were then characterized genotypically by PFGE and rep-PCR. Salmonella DNA fingerprint was analyzed, and dendrogram was generated by Bionumerics software program version 3.5. The results of discriminatory power from PFGE were 0.43, 0.47, 0.85 and 0.9 at similarity index 80%, 85%, 90% and 95% respectively and discriminatory power from rep-PCR were0.55,0.69,0.88and 0.95at similarity index80%,85%,90% and 95% respectively.
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enterica isolates. Journal of microbiological methods, 81(1), 11-16.
CDC U.S. Centers for Disease Control and Prevention (2000). Standardized Molecular Subtyping of Foodborne Bacterial Pathogens by Pulsed-Field Gel Electrophoresis. Atlanta, GA.
DeBusser,E.V., Maes, D., Houf,K., Dewulf, J., Imberechts,H.,Bertrand,S., & De Zutter,L. (2011). Detection and characterization of Salmonella in lairage,on pig carcasses and intestines in five
slaughterhouses. International journalof food microbiology, 145(1), 279-286.
Gunawardana, G. A., Townsend, K. M., & Frost, A. J.(2000). Molecular characterisation of avian PasteurellamultocidaisolatesfromAustraliaand Vietnam by REP-PCR and PFGE. Veterinary
microbiology, 72(1-2), 97-109.
Ibarra, J. A., & Steele-Mortimer, O. (2009).Salmonella--the ultimate insider. Salmonella virulence factors that modulate intracellular survival. Cellular microbiology, 11(11), 1579-1586.
Kiliç, A.,Senses, Z., Aydoğan, H., & Başustaoglu, A. (2006). [Molecular analysis of vancomycin-resistant enterococci isolated from clinical samples]. Mikrobiyoloji bülteni, 40(4), 295–299.
Kilic, A., Bedir, O., Kocak, N., Levent, B., Eyigun, C. P.,Tekbas, O. F., . . . Basustaoglu, A. C. (2010).AnalysisofanoutbreakofSalmonellaenteritidis byrepetitive-sequence-based PCRand pulsed-
field gelelectrophoresis. Internalmedicinejournal , 49(1), 31-36.
Lin, C. W., Chiou, C. S., Chang, Y. C., & Yang, T. C. (2008).Comparisonof pulsed-field gelelectrophoresis and threerep-PCR methods forevaluating the genetic relatedness of Stenotrophomonas
maltophilia isolates. Letters in applied microbiology, 47(5), 393-398.
Liu,B., Zhang,L., Zhu, X.,Shi, C., Chen, J.,Liu, W., . . .Shi,X. (2011). PCR identification of Salmonella serogroups based onspecific targetsobtained bycomparativegenomics. International journal
of food microbiology, 144(3), 511-518.
Magistrali, C., Dionisi, A. M., De Curtis,P., Cucco,L.,Vischi,O., Scuota, S., . . . Pezzotti, G. (2008).Contamination of Salmonella spp. in a pig finishing herd, from the arrival of the animals to the slaughterhouse. Research in Veterinary Science, 85(2), 204-207.
Nassonova,E.S. (2008).Pulsed field gelelectrophoresis:Theory, instruments and application. Cell and Tissue Biology, 2(6), 557–565.
Pasanen, T., Kotila, S. M., Horsma, J., Virolainen, A.,Jalava, J., Ibrahem, S., . . . Tissari, P. (2011).Comparisonof repetitiveextragenic palindromic sequence-based PCR with PCR ribotyping and
pulsed-field gel electrophoresis in studying the clonality of Clostridium difficile. Clinical Microbiology and Infection, 17(2), 166-175.
Patchanee,P., Molla,B., White, N.,Line, D.E., & Gebreyes,W. A. (2010).TrackingSalmonellacontamination in various watersheds and phenotypic and genotypic diversity.Foodborne pathogensand
disease, 7(9), 1113–1120.
Ross,T.L., Merz, W. G.,Farkosh, M., & Carroll,K. C. (2005).Comparison of an automated repetitive sequence-based PCRmicrobial typingsystemto pulsed-field gelelectrophoresis foranalysisof
outbreaksofmethicillin-resistantStaphylococcusaureus. Journalof Clinical Microbiology,43(11),5642-5647.
Severiano, A.,Pinto,F.R.,Ramirez, M., Carrico, J. A. (2011).Adjusted Wallace Coefficient as a measure of congruence between typing methods.Journal of Clinical Microbiology, 49(11),3997-4000.
Severiano, A., Carrico, J. A. (2011). Comparing partition.Retrieved from https://darwin.phyloviz.net/ComparingPartitions/index.php?link=Tool Stepan,R. M.,Sherwood, J.S.,Petermann,S.R., & Logue,
C. M. (2011). Molecular and comparative analysisofSalmonellaentericaSenftenbergfrom humans and animals using PFGE, MLST and NARMS. BMC Microbiol, 11, 153.
Universityof the Basque Country. (2013). Discriminatory Power Calculator.Retrieved fromhttps://insilico.ehu.es/mini_tools/discriminatory_power/
Wattiau,P.,Boland, C., & Bertrand,S. (2011). Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives. Applied and environmental microbiology, 77
Weigel,R. M., Qiao,B.,Teferedegne,B.,Suh, D.K.,Barber,D. A., Isaacson, R. E., & White, B. A. (2004).Comparisonof pulsed field gelelectrophoresis and repetitive sequence polymerase chain reactionasgenotypingmethods for detectionof genetic diversity and inferring transmission of Salmonella.Veterinary microbiology,100(3-4),205-217.