Discriminatory power and genotypic characterization of salmonella by PFGE and rep-PCR

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Pakamas Sukroongruang
Raweewan Rungrudeesombatkit
Nipa Choksajjawatee
Sarinya Pornaem
Phacharaporn Boonkhot
Pakpoom tadee
Prapas Patchanee
Panuwat Yamsakul

Abstract

AbstractThis study wasaimed tocomparea discriminatory powerand to differentiateSalmonella Rissen and Salmonella Typhimurium DNA fingerprint by 2 molecular genotyping methods: Pulsed-Field Gel Electrophoresis (PFGE) and Repetitive Element Sequence-based PCR (rep-PCR). Salmonellaisolates from pig farms in Chiang Mai and Lampoon province, including 14 Salmonella Rissen and 16 Salmonella Typhimurium isolates samples were included in the study. Salmonella serotypes were previously confirmed by National Salmonellae and Shigellae Center, Department of Medical Sciences, Ministry of Public Health, Thailand. All samples were then characterized genotypically by PFGE and rep-PCR. Salmonella DNA fingerprint was analyzed, and dendrogram was generated by Bionumerics software program version 3.5. The results of discriminatory power from PFGE were 0.43, 0.47, 0.85 and 0.9 at similarity index 80%, 85%, 90% and 95% respectively and discriminatory power from rep-PCR were0.55,0.69,0.88and 0.95at similarity index80%,85%,90% and 95% respectively.

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Sukroongruang, P., Rungrudeesombatkit, R., Choksajjawatee, N., Pornaem, S., Boonkhot, P., tadee, P., Patchanee, P., & Yamsakul, P. (2014). Discriminatory power and genotypic characterization of salmonella by PFGE and rep-PCR. Veterinary Integrative Sciences, 12(1), 19–29. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/146664
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Research Articles

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