The relationship between the plasma serotonin level and degenerative mitral valve disease in dogs

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Peechanit Kamkampol
Thatawan Kaewsakorn
Veerasak Punyapornwithaya
Chavalit Boonyapakorn


Degenerative mitral valve disease (DMVD) is a most common cardiac disease in dogs whichareusually found inold small breed dogs. Several factors can bethecauseof degeneration and serotonin is the one. In the present study, serotonin (5-hydroxytryptamine, 5HT) signaling can inducevalvular interstitialcell (VIC) differentiationand myxomatousvalve damage, but theetiology of this disease is unclear. The purpose of this study was to investigate the relationship between plasma serotonin and degenerative mitral valve disease in dogs. Dogs were divided into a normal group (n=7) and a treatment group (n=23). The treatment group was classified by the severity of the clinical signs in class I, II, III and IV group Blood samples were collected for an analysis of plasma serotonin level. The plasma serotonin level from varies group were compared by using the generalized linear mixed model. The results revealed that the plasma serotonin level in each group were different, the tendency of plasma serotonin level decreased when the severity of the disease was increased (P<0.05). The plasma serotonin level in class I group was higher than class II, III and IVgroups (P<0.05) and class I and II groupshad ahigher plasma serotoninlevel thanclass III and IV groups (P<0.05).


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Kamkampol, P., Kaewsakorn, T., Punyapornwithaya, V., & Boonyapakorn, C. (2014). The relationship between the plasma serotonin level and degenerative mitral valve disease in dogs. Veterinary Integrative Sciences, 12(1), 41–48. Retrieved from
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Arndt, J. W., Reynolds, C. A., Singletary, G. E., Connolly, J. M., Levy, R. J., & Oyama, M. A. (2009). Serum serotonin concentrations in dogs with degenerative mitral valve disease. Journal of Veterinary Internal Medicine / American College of Veterinary Internal Medicine, 23(6), 1208–1213. doi:10.1111/j.1939-1676.2009.0378.x

Aupperle, H., März, I., Thielebein, J., Kiefer, B., Kappe, A., & Schoon, H.-A. (2009). Immunohistochemical characterization of the extracellular matrix in normal mitral valves and in chronic valve disease (endocardiosis) in dogs. Research in Veterinary Science, 87(2), 277–283. doi:10.1016/j.rvsc.2009.01.009

Aupperle, H., Thielebein, J., Kiefer, B., März, I., Dinges, G., & Schoon, H.-A. (2009). An immunohistochemical study of the role of matrix metalloproteinases and their tissue inhibitors in chronic mitral valvular disease (valvular endocardiosis) in dogs. Veterinary Journal (London, England: 1997), 180(1), 88–94. doi:10.1016/j.tvjl.2007.11.011

Best, J., Nijhout, H. F., & Reed, M. (2010). Serotonin synthesis, release and reuptake in terminals: a mathematical model. Theoretical Biology & Medical Modelling, 7, 34. doi:10.1186/1742-4682-7-34

Black, A., French, A. T., Dukes-McEwan, J., & Corcoran, B. M. (2005). Ultrastructural morphologic evaluation of the phenotype of valvular interstitial cells in dogs with myxomatous degeneration of the mitral valve. American Journal of Veterinary Research, 66(8), 1408–1414.

Disatian, S., Ehrhart, E. J., 3rd, Zimmerman, S., & Orton, E. C. (2008). Interstitial cells from dogs with naturally occurring myxomatous mitral valve disease undergo phenotype transformation. The Journal of Heart Valve Disease, 17(4), 402–411; discussion 412.

Disatian, S., Lacerda, C., & Orton, E. C. (2010). Tryptophan hydroxylase 1 expression is increased in phenotype-altered canine and human degenerative myxomatous mitral valves. The Journal of Heart Valve Disease, 19(1), 71–78.

Disatian, S., & Orton, E. C. (2009). Autocrine serotonin and transforming growth factor beta 1 signaling mediates spontaneous myxomatous mitral valve disease. The Journal of Heart Valve Disease, 18(1), 44–51.

Erling, P., & Mazzaferro, E. M. (2008). Left-sided congestive heart failure in dogs: pathophysiology and diagnosis. Compendium (Yardley, PA), 30(2), 79–90; quiz 91.

Fuenmayor, L. D., & García, S. (1984). The effect of fasting on 5-hydroxytryptamine metabolism in brain regions of the albino rat. British Journal of Pharmacology, 83(2), 357–362. doi:10.1111/j.1476-5381.1984.tb16495.x

Hadian, M., Corcoran, B. M., Han, R. I., Grossmann, J. G., & Bradshaw, J. P. (2007). Collagen Organization in Canine Myxomatous Mitral Valve Disease: An X-Ray Diffraction Study. Biophysical Journal, 93(7), 2472–2476. doi:10.1529/biophysj.107.107847

Han, R. I., Black, A., Culshaw, G., French, A. T., & Corcoran, B. M. (2010). Structural and cellular changes in canine myxomatous mitral valve disease: an image analysis study. The Journal of Heart Valve Disease, 19(1), 60–70.

Jian, B., Xu, J., Connolly, J., Savani, R. C., Narula, N., Liang, B., & Levy, R. J. (2002). Serotonin Mechanisms in Heart Valve Disease I. The American Journal of Pathology, 161(6), 2111–2121.

Jose Pinheiro, Douglas Bates, Saikat DebRoy, Deepayan Sarkar and the R Development Core Team (2013). nlme: Linear and Nonlinear Mixed Effects Models. R package version 3.1-111.

Kittleson, M. D., & Kienle, R. D. (1998). Myxomatous atrioventricular valvular degeneration. Small Animal Cardiovascular Medicine. Mosby, St. Louis

Kvart, C., Häggström, J., Pedersen, H. D., Hansson, K., Eriksson, A., Järvinen, A.-K., Corfitzen, J. (2002). Efficacy of enalapril for prevention of congestive heart failure in dogs with myxomatous valve disease and asymptomatic mitral regurgitation. Journal of Veterinary Internal Medicine / American College of Veterinary Internal Medicine, 16(1), 80–88.

Levy, R. J. (2006). Serotonin Transporter Mechanisms and Cardiac Disease. Circulation, 113(1), 2–4. doi:10.1161/CIRCULATIONAHA.105.593459

Ljungvall, I., Höglund, K., Lilliehöök, I., Oyama, M. A., Tidholm, A., Tvedten, H., & Häggström, J. (2013). Serum serotonin concentration is associated with severity of myxomatous mitral valve disease in dogs. Journal of Veterinary Internal Medicine / American College of Veterinary Internal Medicine, 27(5), 1105–1112. doi:10.1111/jvim.12137

Oyama, M. A., & Levy, R. J. (2010). Insights into serotonin signaling mechanisms associated with canine degenerative mitral valve disease. Journal of Veterinary Internal Medicine / American College of Veterinary Internal Medicine, 24(1), 27–36. doi:10.1111/j.1939-1676.2009.0411.x

R Core Team (2013). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Retrieve from;

Rajamannan, N. M., Caplice, N., Anthikad, F., Sebo, T. J., Orszulak, T. A., Edwards, W. D., Schwartz, R. S. (2001). Cell proliferation in carcinoid valve disease: a mechanism for serotonin effects. The Journal of Heart Valve Disease, 10(6), 827–831.

Russell V. Lenth (2013). lsmeans: Least-squares means. R package version 1.10-01. Retrieve from;

Wey, A., Deborah, C., Dac, V., & Kate, H. (2009). Valvular Heart Disease. Small Animal Critical Care Medicine. Saint Louis: W.B. Saunders.

Xu, J., Jian, B., Chu, R., Lu, Z., Li, Q., Dunlop, J., Liang, B. (2002b). Serotonin mechanisms in heart valve disease II: the 5-HT2 receptor and its signaling pathway in aortic valve interstitial cells. The American Journal of Pathology, 161(6), 2209–2218. doi:10.1016/S0002-9440(10)64497-5