A survey on blood parasites of birds in Chiang Mai province

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Nithidol Buranapim
Pariya Chaiwisit
Attawit Wangkawan
Saruda Tiwananthagorn


Blood parasites are one of the most important bird diseases. Although, it is not the main reason that causes death in birds, it interferes with vital body function. This study providedthe occurrence of blood parasites in birds in Chiang maiProvince. Blood samples were collected from the 111 birds: red-backed sea eagle 1.8%,rock dove 46.8%, red junglefowl 12.6%, spotted wood-owl 0.9%, pheasant 11.7%, peafowl 1.8%, purple swamphen 0.9%, spotted dove 0.9%, common myna 2.7%, tree sparrow 5.4%, red-whiskered bulbul 11.7% and spotted owlet 2.7%.Blood was drawn from the brachial wing vein, stored in capillary tubes and excess blood was stored in EDTA microcentrifuge tubes. Samples were centrifuged to evaluate Packed cell volume (PCV) and Woo’s technique was performed. To identify other blood parasites, thin blood smear was created, stained with Giemsa’s stain and observed under all microscopic magnifications. From 111 samples, 54 samples were positive (48.65%). 18 samples were positive for more than one species of blood parasites. Four group of blood parasites were detected: Microfilaria 35.14% (39/111),Haemoproteusspp. 17.12% (19/111), Leucocytozoonspp. 8.11% (9/111), and Trypanosomaspp. 4.50% (5/111). Birds that are positive to blood parasites can have normal PCV and low PCV values. They might appear to be as healthy as normal birds and therefore act as a carrier. Vector-borne parasites should be study in the future to understand blood parasite distribution.

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Buranapim, N., Chaiwisit, P., Wangkawan, A., & Tiwananthagorn, S. (2019). A survey on blood parasites of birds in Chiang Mai province. Veterinary Integrative Sciences, 17(1), 65–73. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/150097
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Atkinson, C.T., Thomas, N.J., Hunter, D.B. (Eds.), 2008. Parasitic diseases of wild birds. Wiley-Blackwell, Ames, Iowa.
Bartlett, C.M., 2008. Avian Filarioid Nematodes of the World, in: Wildlife Disease Association. Presented at the Annual International Conference 2008, Edmonton, Canada.
Bennett, G.F., 1970. Simple techniques for making avian blood smears. Can. J. Zool. 48, 585–586. doi:10.1139/z70-098
Campbell, T.W., Ellis, C., 2007. Avian and exotic animal hematology and cytology, 3rd ed. ed. Blackwell Pub, Ames, Iowa.
Chanittawong, W., Chaipakdee, M., 2006. Migratory birds of Thailand. Wildlife Research Division, Department of national parks, wildlife and plant conservation. Wildlife Yearbook, Vol.7
Donovan, T.A., M. Schrenzel, T.A. Tucker, A.P. Pessier and I.H. Stalis,2008. Hepatic Hemorrhage, Hemocoelom and Sudden Death Due to Haemoproteus Infection in Passerine Birds: Eleven Cases.J.Vet Diagn Invest. 20(3): 304-313.
Dunn, J.C., Goodman, S.J., Benton, T.G., Hamer, K.C., 2013. Avian blood parasite infection during the non-breeding season: an overlooked issue in declining populations? BMC Ecology 13, 30. doi:10.1186/1472-6785-13-30
Garamszegi, L.Z., 2010. The Sensitivity of Microscopy and PCR-Based Detection Methods Affecting Estimates of Prevalence of Blood Parasites in Birds. J. Parasitology 96, 1197–1203. doi:10.1645/GE-2531.1
Pholpark, M., Pholpark, S., 2013. Trypanosoma evansi infections in Northeastern region of Thailand, Thai-NIAH eJournal, V8 N2: 32-55
Prompiram, P., Kaewviyudth, S., Sukthana, Y., Rattanakorn, P., 2015. Study of Morphological Characteristic and Prevalence of Haemoproteus Blood parasite in Passerines in Bung Boraphet. Thai J Vet Med. 45(3), 399-409.
Quillfeldt, P., Arriero, E., Martínez, J., Masello, J.F., Merino, S., 2011. Prevalence of blood parasites in seabirds - a review. Frontiers in Zoology 8, 26. doi:10.1186/1742-9994-8-26
Ritchie, B.W., Harrison, G.J., Harrison, L.R. (Eds.), 1994. Avian medicine: principles and application. Wingers Pub, Lake Worth, Fla. pp 176-198
Samour, J. (Ed.), 2016. Avian medicine, Third edition. ed. Elsevier, St. Louis, Missouri.
Sakas, P.S., Bauck, L., 2002. Essentials of avian medicine: a guide for practitioners, 2nd ed. American Animal Hospital Association Press, Lakewood, Colo.
Savage, A.F., Robert, V., Goodman, S.M., Raharimanga, V., Raherilalao, M.J., Andrianarimisa, A., Ariey, F., Greiner, E.C., 2009. Blood parasites in birds from Madagascar. J. Wild. Dis. 45, 907–920. doi:10.7589/0090-3558-45.4.907
Takang, P., Pikulkaew, S., Awaiwanont, N., Numee, S., 2017. Prevaleance and risk factors of blood parasites infection in backyard chickens in Chiang Mai. Chiang Mai Veterinary Journal, 157–167. doi:10.14456/cmvj.2017.15
Worasing, R., Kongkeaw, W., Tiptara, A., Anant, S., 2001. Leucocytoxoonosis with Avian Malaria in Layer Chicken and Treatment. The 39th Kasetsart University Annual Conference ,557-563