Multi-drug resistance of aerobic bacteria from open fractures in dogs and cats

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Luddawon Somrup
Areerath Akatvipat
Duangporn Pichpol
Kannika Nalampang


Antimicrobial resistance patterns provide initial information for treatment planning. The purpose of this study was to identify aerobic bacterial contamination and antimicrobial drugs resistance patterns from open fracture by collected sampling from 25 dogs and 13 cats. Samples were obtained from open fracture wound at three points of time, the first point were collected immediately before the wound was cleaned, the second point were sampling after the wound was cleaned and the third point were collected after complete surgery, before the wound was closed. The samples were identified for bacterial contamination and antimicrobial resistance pattern. Seventeen bacterial species was identified and the most common bacteria was Pseudomonas spp. There was a high variation of aerobic bacterial identification and antimicrobial resistance patterns results. While the bacterial identification and resistance result is not achieved, the first-line antibiotics for bone fracture were recommended. Once the laboratory results obtain, antibiotic selection should base on each patient result


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Somrup, L., Akatvipat, A., Pichpol, D., & Nalampang, K. (2018). Multi-drug resistance of aerobic bacteria from open fractures in dogs and cats. Veterinary Integrative Sciences, 16(3), 173–182. Retrieved from
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Cheng, Q., Zhang, X. F., Di, D. H., Zhao, G. Y., & Cui, X. W. (2015). Efficacy of different irrigation solutions on the early debridement of open fracture in rats. Exp Ther Med, 9(5), 1589-1592. doi:10.3892/etm.2015.2325

Gustilo, R. B., & Anderson, J. T. (1976). Prevention of infection in the treatment of one thousand and twenty-five open fractures of long bones: retrospective and prospective analyses. J Bone Joint Surg Am, 58(4), 453-458.

Hauser, C. J., Adams, C. A., Jr., & Eachempati, S. R. (2006). Surgical infection society guideline: prophylactic antibiotic use in open fractures: an evidence-based guideline. Surg Infect, 7(4), 379-405. doi:10.1089/sur.2006.7.379

Isaac, S. M., Woods, A., Danial, I. N., & Mourkus, H. (2016). Antibiotic prophylaxis in adults with open tibial fractures: what is the evidence for duration of administration? a systematic review. J Foot Ankle Surg, 55(1), 146-150. doi:10.1053/j.jfas.2015.07.012

Jackson, L. C., & Pacchiana, P. D. (2004). Common complications of fracture repair. Top Companion Anim Med, 19(3), 168-179. doi:10.1053/j.ctsap.2004.09.008

Millard, R. P., Towle, H.A. (2011). Open fractures in Veterinary surgery (K. M. Tobias, Johnston, S.A. Ed.): Elsevier.

Millard, R. P., & Weng, H. Y. (2014). Proportion of and risk factors for open fractures of the appendicular skeleton in dogs and cats. J Am Vet Med Assoc, 245(6), 663-668. doi:10.2460/javma.245.6.663

Ness, M. G. (2006). Treatment of inherently unstable open or infected fractures by open wound management and external skeletal fixation. J Small Anim Pract, 47(2), 83-88. doi:10.1111/j.1748-5827.2006.00034.x

Neubauer, T., Bayer, G. S., & Wagner, M. (2006). Open fractures and infection. Acta Chir Orthop Traumatol Cech, 73(5), 301-312.

Otchwemah, R., Grams, V., Tjardes, T., Shafizadeh, S., Bathis, H., Maegele, M., . . . Probst, C. (2015). Bacterial contamination of open fractures - pathogens, antibiotic resistances and therapeutic regimes in four hospitals of the trauma network Cologne, Germany. Injury, 46 Suppl 4, S104-108. doi:10.1016/s0020-1383(15)30027-9

Patzakis, M. J., Bains, R. S., Lee, J., Shepherd, L., Singer, G., Ressler, R., . . . Holtom, P. (2000). Prospective, randomized, double-blind study comparing single-agent antibiotic therapy, ciprofloxacin, to combination antibiotic therapy in open fracture wounds. J Orthop Trauma, 14(8), 529-533.

Perry, K. (2016). CPD article: Management of open fractures: part 2 (Vol. 21).

PerryBVM, K. L., & MRCVS, S. C. D. F. (2016). Management of open fractures: part 1. Companion Animal, 21(3), 165-170. doi:10.12968/coan.2016.21.3.165

Robinson, D., On, E., Hadas, N., Halperin, N., Hofman, S., & Boldur, I. (1989). Microbiologic flora contaminating open fractures: its significance in the choice of primary antibiotic agents and the likelihood of deep wound infection. J Orthop Trauma, 3(4), 283-286.

Rodriguez, L., Jung, H. S., Goulet, J. A., Cicalo, A., Machado-Aranda, D. A., & Napolitano, L. M. (2014). Evidence-based protocol for prophylactic antibiotics in open fractures: improved antibiotic stewardship with no increase in infection rates. J Trauma Acute Care Surg, 77(3), 400-407; discussion 407-408; quiz 524. doi:10.1097/ta.0000000000000398

Siqueira, E. G., Rahal, S. C., Ribeiro, M. G., Paes, A. C., Listoni, F. P., & Vassalo, F. G. (2014). Exogenous bacterial osteomyelitis in 52 dogs: a retrospective study of etiology and in vitro antimicrobial susceptibility profile (2000-2013). Vet Q, 34(4), 201-204. doi:10.1080/01652176.2014.974000