Pathological microscopy in liver parenchyma of gray-eel catfish, Plotosus canius from Ang-Sila area, Chonburi Province, Thailand
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Dec 24, 2019
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Histopathology Liver Plotosus canius Thailand
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Abstract
This is the first discovered abnormal liver of gray-eel catfish (Plotosus canius), which was collected from the Ang-Sila area, Chonburi Province, Thailand. Anatomically, the liver tissue was observed to be both shrunken in overall appearance and with almost white colorization in all lobules. Based on histological and histochemical observations, the liver parenchyma in this species was clearly altered. A large number of vacuolar structures in the hepatocyte were shown as empty spaces (H&E stained), but these structures appeared after the ORO reaction as orange color, indicative of hepatocellular lipidosis. The loss of glycogen in hepatocytes was also observed by using PAS reaction. In addition, necrosis with poor sinusoids structure was occasionally seen in some liver areas. All abnormalities seen in these liver samples might be related to reduced functionality as well as health status of P. canius ; further, the altered health of these fish might interfere with other animals/ humans within ecological dynamic., Overall, results shown here demonstrate the importance of anatomical and histological studies and we suggest such studies should be incorporated into water quality monitoring in the Ang-Sila zone of Thailand and represent an important consideration for other locations around the world.
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Senarat, S., Kettratad, J., Gerald Plumley, F., Wangkulangkul, S., Jiraungkoorskul, W., Boonyoung, P., & Poolprasert, P. (2019). Pathological microscopy in liver parenchyma of gray-eel catfish, Plotosus canius from Ang-Sila area, Chonburi Province, Thailand. Veterinary Integrative Sciences, 17(3), 255–261. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/222964
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References
Adams, S.M. 2002. Biological indicators of aquatic ecosystem stress. Am. Fish. Soc. 3, 104–112.
Anderson, M.J., Cacela, D., Beltman, D., Teh, S.J., Okihiro, M.S., Hinton, D.E., Denslow, N., Zelikoff, J.T. 2003 Biochemical and toxicopathic biomarkers assessed in smallmouth bass recovered from a polychlorinated biphenyl-contaminated river. Biomark. 8, 371– 393.
Bancroft, J.D. and Gamble, M. 2002. Theory and Practice of Histological Techniques, Churchill Livingstone, London, UK.
Culling, C.F.A. 1963. Handbook of Histopathological Technique, 2nd. Butterworth, London, U.K.
Dietrich, D.R. and Krieger, H.O. 2009. Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish. John Wiley and Sons, New Jersey, U.S.A.
Diniz, M.S., de Matos, A.P., Lourenço, J., Castro, L., Peres, I., Mendonça., E., Picado. A. 2013. Liver Alterations in Two Freshwater Fish Species (Carassius auratus and Danio rerio) Following Exposure to Different TiO2 Nanoparticle Concentration. Microsc. Microanal. 19, 1131–1140.
Fatma, A.S.M. 2009. Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World J. Fish Mar. Sci. 1, 29–39.
Genc, E., Yilmaz, E., Akyurt, I. 2005. Effects of dietary fish oil, soy-acid oil, and yellow grease on growth and hepatic lipidosis of hybrid tilapia fry. Isr. J. Aquacult.–Bamid. 57(2), 90–96.
Genten, F., Terwinghe, E., Danguy, A. 2008. Atlas of Fish Histology. Science Publishers, Enfield. New Hampshire, U.S.A.
Greenfield, B.K., Teh, S.J., Ross, J.R., Hunt, J., Zhang, G., Davis, J.A., Ichikawa, G., Crane, D., Hung, S.S., Deng, D., The, F.C., Green, P.G. 2008. Contaminant Concentrations and Histopathological Effects in Sacramento Splittail (Pogonichthys macrolepidotus). Arch Environ Contam Toxicol. 55, 270–281.
Hendricks, J.D., Meyers, T.R., Shelton, D.W. 1984. Histological progression of hepatic neoplasia in rainbow trout (Salmo gairdneri). J. Natl. Cancer Inst. 65, 321–336.
Hinton, D.E., Laure´n, D.J. 1990. Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure. In: McCarthy, J.F., Shugart, L.R. (Eds) Biomarkers of Environmental Contamination. Lewis, Boca Raton, FL, pp. 17–57.
Hinton, D.E., Baumann, P.C., Gardner, G.R., Hawkins, W.E., Hendricks, J.D., Murchelano, R.A., Okihiro, M.S. 1992. Histopathologic Biomarkers. Biochemical, Physiological, and Histological Markers of Anthropogenic Stress. Biomarkers, Lewis Publishers, Boca Raton, FL, pp. 155–209.
Hinton, D.E., Segner, H., Braunbeck, T. 2001. Toxic responses of the liver. In Target Organ Toxicity in Marine and Freshwater Teleosts. In: Schlenk, D., Benson, W.H, (Eds.), Taylor and Francis, London, pp. 224–268.
Humason, G.L. 1979. Animal Tissue Techniques, vol 4. San Francisco, Freeman.
Keawjam, S. 2014. Gut content analysis of Plotosus canius (Hamilton, 1822) from Ang-Sila area, Chonburi Province, Thailand. Senior project, Faculty of Science, Chulalongkorn University.Nikalje, S.B., Muley, D.V., Angadi, S.M. 2012. Histopathological changes in gills of a freshwater major carp, Labeo rohita after acute and chronic exposure to textile mill effluent (tme). Int. J. Environ. Sci. 3, 108–118.
Robertson, J.C., Bradley, T.M. 1992. Liver ultrastructure of juvenile Atlantic salmon (Salmo salar). J. Morph. 211, 41–54.
Ruiz-Ramírez, J.A., Ramírez-Ayala, E., Tintos-Gómez, A., Salas-Villaseñor, C., García-Márquez, L.J., Borja-Gómez, I., Sepúlveda-Quiroz, C.A., Lezama-Cervantes, C., Morales-Blake, A.R. 2019. Hepatocellular steatosis as a response to nutritional stressors in Lutjanus guttatus (Steindachner, 1869) grown in floating cages: a case study. Lat. Am. J. Aquat. Res. 47(4), 709–715.
Sanad, S.M., Gamaal, M.A.A., Hemmaid, D.K. 2015. Histopathological Changes in the Liver of the Nile Fish Oreochromis niloticus Fed on the Blue-Green Algae Microcystis aeruginosa under Laboratory Conditions. International Conference on Biological, Civil and Environmental Engineering (BCEE-2015) Feb. 3–4, 2015 Bali (Indonesia).
Schrank, C.S., Cormier, S.M., Blazer, V.S. 1997. Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin. J. Great Lakes Res. 23, 119–130.
Senarat, S., Kettratad, J., Poolprasert, P. Jiraungkoorskul, W., Yenchum, W. 2015. Histopathological findings of liver and kidney tissues of the yellow mystus, Hemibagrus filamentus (Fang and Chaux, 1949), from the Tapee River, Thailand. Songklanakarin Journal of Science and Technology (SJST). 37, 1–5.
Senarat, S., Kettratad, J., Tipdomrongpong, S., Pengsakul, T., Jiraungkoorskul, W., Boonyoung, P., & Huang, S. (2018). Histopathology of kidney and liver in the captive broodstock (Rastrelliger brachysoma) during its juvenile stage. Vet. Integr. Sci. 16(2), 87–93.
Teh, S.J., Adams, S.M., Hinton, D.E. 1997. Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquat. Toxicol. 37, 51-70.
Yilmaz, E., Genc, E. 2006. Effects of Alternative Dietary Lipid Sources (Soy-acid oil and Yellow grease) on Growth and Hepatic Lipidosis of Common Carp (Cyprinus carpio) Fingerling: A Preliminary Study. Turk. J. Fish. Aquat. Sc.6, 37–42.
Yilmaz, E.C.E., Akyurt, I. 2005. Effects of Dietary Fish Oil, Soy-Acid Oil, and Yellow Grease on Growth and Hepatic Lipidosis of Hybrid Tilapia Fry. Isr. J. Aquacult.–Bamid. 57, 90–96.
Anderson, M.J., Cacela, D., Beltman, D., Teh, S.J., Okihiro, M.S., Hinton, D.E., Denslow, N., Zelikoff, J.T. 2003 Biochemical and toxicopathic biomarkers assessed in smallmouth bass recovered from a polychlorinated biphenyl-contaminated river. Biomark. 8, 371– 393.
Bancroft, J.D. and Gamble, M. 2002. Theory and Practice of Histological Techniques, Churchill Livingstone, London, UK.
Culling, C.F.A. 1963. Handbook of Histopathological Technique, 2nd. Butterworth, London, U.K.
Dietrich, D.R. and Krieger, H.O. 2009. Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish. John Wiley and Sons, New Jersey, U.S.A.
Diniz, M.S., de Matos, A.P., Lourenço, J., Castro, L., Peres, I., Mendonça., E., Picado. A. 2013. Liver Alterations in Two Freshwater Fish Species (Carassius auratus and Danio rerio) Following Exposure to Different TiO2 Nanoparticle Concentration. Microsc. Microanal. 19, 1131–1140.
Fatma, A.S.M. 2009. Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World J. Fish Mar. Sci. 1, 29–39.
Genc, E., Yilmaz, E., Akyurt, I. 2005. Effects of dietary fish oil, soy-acid oil, and yellow grease on growth and hepatic lipidosis of hybrid tilapia fry. Isr. J. Aquacult.–Bamid. 57(2), 90–96.
Genten, F., Terwinghe, E., Danguy, A. 2008. Atlas of Fish Histology. Science Publishers, Enfield. New Hampshire, U.S.A.
Greenfield, B.K., Teh, S.J., Ross, J.R., Hunt, J., Zhang, G., Davis, J.A., Ichikawa, G., Crane, D., Hung, S.S., Deng, D., The, F.C., Green, P.G. 2008. Contaminant Concentrations and Histopathological Effects in Sacramento Splittail (Pogonichthys macrolepidotus). Arch Environ Contam Toxicol. 55, 270–281.
Hendricks, J.D., Meyers, T.R., Shelton, D.W. 1984. Histological progression of hepatic neoplasia in rainbow trout (Salmo gairdneri). J. Natl. Cancer Inst. 65, 321–336.
Hinton, D.E., Laure´n, D.J. 1990. Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure. In: McCarthy, J.F., Shugart, L.R. (Eds) Biomarkers of Environmental Contamination. Lewis, Boca Raton, FL, pp. 17–57.
Hinton, D.E., Baumann, P.C., Gardner, G.R., Hawkins, W.E., Hendricks, J.D., Murchelano, R.A., Okihiro, M.S. 1992. Histopathologic Biomarkers. Biochemical, Physiological, and Histological Markers of Anthropogenic Stress. Biomarkers, Lewis Publishers, Boca Raton, FL, pp. 155–209.
Hinton, D.E., Segner, H., Braunbeck, T. 2001. Toxic responses of the liver. In Target Organ Toxicity in Marine and Freshwater Teleosts. In: Schlenk, D., Benson, W.H, (Eds.), Taylor and Francis, London, pp. 224–268.
Humason, G.L. 1979. Animal Tissue Techniques, vol 4. San Francisco, Freeman.
Keawjam, S. 2014. Gut content analysis of Plotosus canius (Hamilton, 1822) from Ang-Sila area, Chonburi Province, Thailand. Senior project, Faculty of Science, Chulalongkorn University.Nikalje, S.B., Muley, D.V., Angadi, S.M. 2012. Histopathological changes in gills of a freshwater major carp, Labeo rohita after acute and chronic exposure to textile mill effluent (tme). Int. J. Environ. Sci. 3, 108–118.
Robertson, J.C., Bradley, T.M. 1992. Liver ultrastructure of juvenile Atlantic salmon (Salmo salar). J. Morph. 211, 41–54.
Ruiz-Ramírez, J.A., Ramírez-Ayala, E., Tintos-Gómez, A., Salas-Villaseñor, C., García-Márquez, L.J., Borja-Gómez, I., Sepúlveda-Quiroz, C.A., Lezama-Cervantes, C., Morales-Blake, A.R. 2019. Hepatocellular steatosis as a response to nutritional stressors in Lutjanus guttatus (Steindachner, 1869) grown in floating cages: a case study. Lat. Am. J. Aquat. Res. 47(4), 709–715.
Sanad, S.M., Gamaal, M.A.A., Hemmaid, D.K. 2015. Histopathological Changes in the Liver of the Nile Fish Oreochromis niloticus Fed on the Blue-Green Algae Microcystis aeruginosa under Laboratory Conditions. International Conference on Biological, Civil and Environmental Engineering (BCEE-2015) Feb. 3–4, 2015 Bali (Indonesia).
Schrank, C.S., Cormier, S.M., Blazer, V.S. 1997. Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin. J. Great Lakes Res. 23, 119–130.
Senarat, S., Kettratad, J., Poolprasert, P. Jiraungkoorskul, W., Yenchum, W. 2015. Histopathological findings of liver and kidney tissues of the yellow mystus, Hemibagrus filamentus (Fang and Chaux, 1949), from the Tapee River, Thailand. Songklanakarin Journal of Science and Technology (SJST). 37, 1–5.
Senarat, S., Kettratad, J., Tipdomrongpong, S., Pengsakul, T., Jiraungkoorskul, W., Boonyoung, P., & Huang, S. (2018). Histopathology of kidney and liver in the captive broodstock (Rastrelliger brachysoma) during its juvenile stage. Vet. Integr. Sci. 16(2), 87–93.
Teh, S.J., Adams, S.M., Hinton, D.E. 1997. Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquat. Toxicol. 37, 51-70.
Yilmaz, E., Genc, E. 2006. Effects of Alternative Dietary Lipid Sources (Soy-acid oil and Yellow grease) on Growth and Hepatic Lipidosis of Common Carp (Cyprinus carpio) Fingerling: A Preliminary Study. Turk. J. Fish. Aquat. Sc.6, 37–42.
Yilmaz, E.C.E., Akyurt, I. 2005. Effects of Dietary Fish Oil, Soy-Acid Oil, and Yellow Grease on Growth and Hepatic Lipidosis of Hybrid Tilapia Fry. Isr. J. Aquacult.–Bamid. 57, 90–96.