Occurrence of extended-spectrum beta-lactamase producing E. coli in broiler farm workers and the farm environment in Chiang Mai-Lamphun, Thailand https://doi.org/10.12982/VIS.2021.002

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Published: Sep 14, 2020
Keywords:
Broiler, E. coli, ESBL, antimicrobial resistance

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Jutamart Rodroo
Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
Montira Intanon
Department of Veterinary Bioscience and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
Khwanchai Kreausukon
Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
Aphisek Kongkaew
Research Administration Section, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
Jeff Bender
School of Public Health, University of Minnesota, Saint Paul, Minneapolis, USA
Nattakarn Awaiwanont
Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand Veterinary Public Health and Food Safety Centre for Asia Pacific (VPHCAP), Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand

Abstract

Antimicrobial resistance has become a major global public health threat. Extended-spectrum beta-lactamase (ESBL) producing E. coli appears as an emergence cause of treatment failure and increase mortality due to limited available effective antimicrobial agents. This study was conducted to determine the occurrence and antimicrobial resistance of ESBL producing E. coli in broilers, farm workers and environment in broiler farms in Chiang Mai-Lamphun, Thailand. The prevalence of ESBL producing E. coli in the broiler farms was 60.4% (29/48). The prevalence of ESBL producing E. coli from boot swabs, farm worker’s rectal swabs, feed and water samples were 43.8%, 55.7%, 12.5% and 2.1%, respectively. All isolates showed susceptible to imipenem and, in contrast, resistant to ampicillin. The results demonstrated high antimicrobial resistant rate to streptomycin (94.3%), gentamicin (86.8%), tetracycline (77.4%), chloramphenicol (66.0%), nalidixic acid (58.5%), and sulfamethoxazole/trimethoprim (56.6%). High percentage (96.2%) of isolates was classified as multidrug resistance (MDR). Thirty-five antimicrobial resistance profiles were identified with AMP-GEN-SXT-NAL-TET-CHL-STR, AMP-GEN-SXT-TET-CHL-STR (14.3%) as the 2 most prevalent profiles. The common resistance profiles between farm workers and broilers was demonstrated. These findings are suggestive for possible transmission between poultry and humans in broiler farms, most likely via close contact. Antimicrobial usage should be strictly controlled together with increase awareness on hygiene practices in broiler farms.

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How to Cite
Rodroo, J., Intanon, M., Kreausukon, K., Kongkaew, A., Bender, J., & Awaiwanont, N. (2020). Occurrence of extended-spectrum beta-lactamase producing E. coli in broiler farm workers and the farm environment in Chiang Mai-Lamphun, Thailand: https://doi.org/10.12982/VIS.2021.002. Veterinary Integrative Sciences, 19(1), 23–35. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/244129
Issue
Vol. 19 No. 1 (2021): January-April
Section
Research Articles
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