Preliminary assessment of probiotic Bacillus subtilis C-3102 in feces: evaluation of their survival after oral supplementation in goats https://doi.org/10.12982/VIS.2021.014

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Watcharapong Choonkham
Axel (A. H.) van Ruitenbeek
Jan Thomas Schonewille
Witaya Suriyasathaporn

Abstract

The objective of the study was to assess the presence of Bacillus subtilis C-3102 in feces after oral supplementation in goats. Six, rumen-fistulated, 3.5 year-old, non-lactating female Saanen goats (average initial body weight of 65 ± 8 kg) were assigned to two treatments 1) a basal diet (CON), and 2) a basal diet supplemented with B. subtilis C-3102 probiotic product (BS) in a cross-over design. Each experimental period lasted 21 days with was preceded by a 20 days adaptation period. On the last day of each experimental period, rumen fluid and fecal samples were collected. Body weights were recorded weekly throughout the experiment. Body weight and rumen pH were found to be similar between dietary treatments. The goats that received BS had higher numbers of B. subtilis C-3102 in fecal samples than CON. It was therefore concluded that supplemental B. subtilis C-3102 met an important precondition of probiotics in that they can survive the passage through the gastrointestinal tract without evident adverse effects. The current result provides a solid basis for future research involving any effects after supplementing probiotic B. subtilis in goats.

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How to Cite
Choonkham, W., van Ruitenbeek , A. (A. H. ., Schonewille , J. T. ., & Suriyasathaporn, W. . (2021). Preliminary assessment of probiotic Bacillus subtilis C-3102 in feces: evaluation of their survival after oral supplementation in goats: https://doi.org/10.12982/VIS.2021.014. Veterinary Integrative Sciences, 19(2), 153–159. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/246119
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Research Articles

References

Choonkham, W., Schonewille, J. T., Bernard, J. K., Suriyasathaporn, W., 2020. Effects of on-farm supplemental feeding of probiotic Bacillus subtilis on milk production in lactating dairy cows under tropical conditions. J. Anim. Feed Sci. 29, 199–205.
Choonkham, W., Suriyasathaporn, W., 2018. Antioxidant capacity of dairy cows after supplementation with dietary probiotic Bacillus subtilis during the transition period. 2018 American Dairy Science Association Annual Meeting. J. Dairy Sci. 101, 354.
CVB (Centraal Veevoederbureau), 2012. Tabellenboek Veevoeding. Productschap Diervoeder, Den Haag.
FAO (Food and Agriculture Organization of the United Nations), 2016. Probiotics in animal nutrition – Production, impact and regulation by Yadav S. Bajagai, Athol V. Klieve, Peter J. Dart and Wayne L. Bryden. FAO, Rome.
Kritas, S. K., Govaris, A., Christodoulopoulos, G., Burriel, A. R., 2006. Effect of Bacillus licheniformis and Bacillus subtilis Supplementation of ewe’s feed on sheep milk production and young lamb mortality. J. Vet. Med. A Physiol. Pathol. Clin. Med. 53, 170–173.
Menegat, M. B., DeRouchey, J. M, Woodworth, J. C., Dritz, S. S., Tokach, M. D., Goodband, R. D., 2019. Effects of Bacillus subtilis C-3102 on sow and progeny performance, fecal consistency, and fecal microbes during gestation, lactation, and nursery periods1,2. J. Anim. Sci. 97, 3920–3937.
Menegat, M. B., DeRouchey, J. M., Woodworth, J. C., Tokach, M. D., Goodband, R. D., Dritz, S. S., 2020. Effects of oral administration of Bacillus subtilis C-3102 to nursing piglets on preweaning growth performance, fecal consistency, and fecal microbes. J. Swine Health Prod. 28, 12–20.
Mousa, S., Elsayed, A., Marghani, B., Ateya, A., 2019. Effects of supplementation of Bacillus spp. on blood metabolites, antioxidant status, and gene expression pattern of selective cytokines in growing Barki lambs. J. Adv. Vet. Anim. Res. 6, 333–340.
Peng, H., Wang, J. Q., Kang, H. Y., Dong, S. H., Sun, P., Bu, D. P., Zhou, L. Y., 2012. Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows. J. Anim. Physiol. Anim. Nutr. 96, 506–512.
Song, D. J., Kang, H. Y., Wang, J. Q., Peng, H., Bu, D. P., 2014. Effect of feeding Bacillus subtilis natto on hindgut fermentation and microbiota of Holstein dairy cows. Asian-Australas. J. Anim. Sci. 27, 495–502.
Souza, V. L., Lopes, N. M., Zacaroni, O. F., Silveira, V. A., Pereira, R. A. N., Freitas, J. A., Almeida, R., Salvati, G. G. S., Pereira, M. N., 2017. Lactation performance and diet digestibility of dairy cows in response to the supplementation of Bacillus subtilis spores. Livest. Sci. 200, 35–39
Sun, P., Li, J., Bu, D., Nan, X., Du, H., 2016. Effects of Bacillus subtilis natto and different components in culture on rumen fermentation and rumen functional bacteria in vitro. Curr. Microbiol. 72, 589–595.
Sun, P., Wang, J. Q., Deng, L. F., 2013. Effects of Bacillus subtilis natto on milk production, rumen fermentation and ruminal microbiome of dairy cows. Animal. 7, 216–222.