The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken https://doi.org/10.12982/VIS.2021.015
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Published:
Jan 25, 2021
Keywords:
Pradu-hangdum chicken, Semen quality, Sperm morphology, Longevity, Freezability
Main Article Content
Abstract
Pradu-hangdum is a distinctive Thai native pure breed chicken. To conserve the pure breed of this chicken species, the artificial insemination was invested. The key to the highly successful output of this technique is high quality of semen. Hence, the objective of the present study is to characterize the Pradu-hangdam sperms and their longevity and freezability in the BHSV extender. The semen of the 30 randomized male chicken were collected. The macroscopic and microscopic examination were used to determine the sperm characters. The results revealed that the Pradu-hangdam sperms contained normal a spiral-shaped head, mid-piece and tail and normal white cream color. The mean number of sperm concentration was 5.24×109 ± 1.54 sperms/mL. The mean volume was 0.22 ± 0.08 mL. The results of longevity in mean total motility of sperm was 85.20%, 56.00% and 36.33% storage for 1, 24, and 48 hours after semen collection in extender, respectively. The longevity of sperm storage in extender decreased significantly at 24 and 48 hours (P<0.05). The freezability of Pradu-hangdam semen significant decreased from 81.45% to 57.02% of motility (P<0.05) but in the range of acceptable result for insemination. In conclusion, this study provides the basic knowledge of sperm characters and their longevity which decreases in relation to the time after collection even though it was preserved and frozen in the BHSV of the acceptable data. Furthermore, the freezing technique and fertility rate should be a further study in the long-term preservation of Pradu-hangdam sperms.
Article Details
How to Cite
Khunkaew, C. ., Patchanee, P. ., Panasophonkul, S., Cao, Z. ., Zhang, Y. ., & Sathanawongs, A. (2021). The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken : https://doi.org/10.12982/VIS.2021.015. Veterinary Integrative Sciences, 19(2), 161–172. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/247021
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Research Articles
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References
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Thurston, R.J., 1995. Storage of poultry semen above freezing for 24–48 hours. In: Bakst, M.R., Wishart, G.J.Eds., Proc. 1st Int. Symp. on Artificial Insemination in Poultry. Poultry Science Association, Savoy, IL, pp. 107–122.
Blanco, J.M., Wildt, D.E., Hofle, U., Voelker, W., Donoghue, A.M.2008. Implementing artificial insemination as an effective tool for conservation of endangered avian species. Theriogenology. 71, 200-213.
Blesbois, E. 2007. Current status in avian semen cryopreservation. World’s Poul. Sci. J. 63, 213-222.
Burrows, W.H. and J.P. Quinn. 1937. The collection of spermatozoa from the domestic fowl and turkey. Poultry Sci. 16, 19-24.
Cole, H.H., Cupps, P.T. 1977. Reproduction in domestic animals. 3rd Edition. New York. Academic press. pp. 195.
Donoghue, A.M., Wishart, G.J. 2000. Storage of poultry semen. Anim. Reprod. Sci. 62: 213–232.
Feyisa SG, Park YM, Lee BR, Jung KM, Choi SB, Cho CY, Han JY. Asian-Australas Journal of Animal Science 2018; 31(8):1160-1168.
Gee GF, Bertschinger H, Donoghue AM, Blanco J, Soley J. Reproduction in nondomestic birds: physiology, semen collection, artificial insemination and cryopreservation. Avian and Poultry Biology Reviews 2004; 15(2): 47-101.
Getachew T. A Review Article of Artificial Insemination in Poultry. World’s Veterinary Journal 2016; 6(1): 25-33.
Giesen, A.F., Sexton, T.J., 1983. Beltsville Poultry Semen Extender. 9. Effect of Storage Temperature on Turkey Semen Held Eighteen Hours. Poult. Sci. 62 (7), 1305-1311.
Hafez ESE. Reproductive in Farm Animals. 3rd ed. Lea and Fibiger, USA. 1974.409.
Kammonkun J, Leotaragul A. Estimation of Genetic parameters for Economic Traits in Thai Native Chicken (Pradu-Hangdum Chiangmai) for Fourteen Generations of Selection. Khon Kaen Agricultural Journal 2015; 43(2):196-199.
Lake PE. The history and future of the cryopreservation of avian germ plasm. Poult Sci 1986; 65:1-15.
Lake, P.E., 1989. Recent progress in poultry reproduction. World’s Poult. Sci. J. 45, 53–59.
Lake, P.E., 1995. Historical perspective of artificial insemination technology. In: Bakst, M.R., Wishart, G.J.(Eds). , Proc. 1st International Symposium on the Artificial Insemination of Poultry. Poultry Science Association, Savoy, IL, pp. 1–20.
Long JA, Kulkarni G. An effective method for improving the fertility of glycerol-exposed poultry semen. Poult Sci 2004; 83:1594-1601.
Mitchell RL, Buckland RB, Kennedy BW. Heritability of Fertility of Frozen and Fresh Chicken Semen and the Relationship between the Fertility of Frozen and Fresh Semen. Poultry Science 1977; 56(4): 1168-1177.
Mocé E, Grasseau I, Blesbois E. Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react. Animal Reproduction Sciences. 2010; 122(3-4): 359-366.
Mohann J, Sharma SK, Kolluri G, Dhama K. History of artificial insemination in poultry, its components and significance. World’s Poultry Science Journal 2018; 74(3): 475-488.
Phianmongkhol S, Wirjantoro TD, Chailungka C, Prathum C, Leotaragul A. Proceeding of the 2nd international seminar on animal industry. 2012; 656-662.
Pongduang U, Leotaragul A, Pianmongkol A, Thakeow P, Jaturasitha S. Journal of Agricultural Research and Extension. 2013; 30(2): 37-46.
Sanchez, Villagran E, Risopatron J, Celis R. Evaluation of nuclear maturity in human spermatozoa obtained by sperm preparation methods. Andrologia 1994; 26(3).173-6.
Seigneurin, F., Grasseau, I., Chapuis, H., Blesbois, E., 2013. An efficient method of guinea fowl sperm cryopreservation. Poultry science 92, 2988-2996.
Sexton, T.J., 1988. Comparison of commercial diluents for holding turkey semen 24 hours at 5oC. Poult. Sci.67, 131–134.
Singh P, Raina VS. Comparative effect of certain additives on preservation of buffalo bull semen at refrigeration temperature. Indian J Prod Manage. 1999;15:74–6.
Tarif AMM, Bhuiyan MMU, Ferdousy RN, Juyena NS, Mollah MBR. Journal of Agriculture and Veterinary Science 2013; 6(5):7-13.
Thurston, R.J., 1995. Storage of poultry semen above freezing for 24–48 hours. In: Bakst, M.R., Wishart, G.J.Eds., Proc. 1st Int. Symp. on Artificial Insemination in Poultry. Poultry Science Association, Savoy, IL, pp. 107–122.