Prevalence and antimicrobial resistance of Salmonella spp. isolated from chicken meat in upper northeastern Thailand https://doi.org/10.12982/VIS.2021.011
Article Sidebar
Main Article Content
Abstract
Foodborne disease associated with Salmonella spp. occurs in some parts of the world, including Thailand. The present study aimed to determine prevalence, antimicrobial resistance, and serotypes of Salmonella spp. isolated from chicken meat in upper northeastern Thailand. A total of 326 swab samples of fresh chicken meat were collected from wet markets in Khon Kaen, U-don Thani, Nong Khai, Loei, Kalasin, Maha Sarakham, and Bueng Kan Provinces, northeastern Thailand, between August and November 2019. All samples were analyzed for Salmonella spp. using the ISO 6579:2002/AMD 1:2007 method. The isolates were tested for antimicrobial susceptibility using the Kirby-Bauer method, and the serotypes of isolates positive were identified. The overall prevalence of Salmonella spp. isolated from chicken meat was 36.2% (118/326), and prevalence in the provinces was as follows: U-don Thani, 70.9%; Bueng Kan, 66.67%; Khon Kaen, 45.9%; Kalasin, 31.25%; Nong Khai, 29.8%; Maha Sarakham, 26.42% and Loei, 12.5%. Resistance was highest for nalidixic acid at 31%, followed by ampicillin (24%), tetracycline (19%), sulfamethoxazole trimethoprim (8%), norfloxacin (5%), ciprofloxacin (4%), amoxicillin (4%), and chloramphenicol (1%). However, all isolates were susceptible to ceftazidime. Twenty-seven serotypes were detected, with the Corvallis, Singapore, Kentucky and Agona serotypes being the most common. Salmonella spp. were detected in a large percentage of the swab samples of chicken meat in every city, indicating a high level of contamination of chicken meat. Given the high resistance of Salmonella strains to some antibiotics, it may be beneficial to find other drugs for salmonellosis treatment and to use antibiotics more wisely.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.
References
Clinical, & Institute, L. S. (2017). Performance standards for antimicrobial susceptibility testing: Clinical and Laboratory Standards Institute Wayne, PA.
Demirbilek, S. K. (2017). Salmonellosis in animals. Salmonella-A Re-emerging Pathogen.
Duc, V. M., Nakamoto, Y., Fujiwara, A., Toyofuku, H., Obi, T., & Chuma, T. (2019). Prevalence of Salmonella in broiler chickens in Kagoshima, Japan in 2009 to 2012 and the relationship between serovars changing and antimicrobial resistance. BMC veterinary research, 15(1), 108.
Hur, J., Jawale, C., & Lee, J. H. (2012). Antimicrobial resistance of Salmonella isolated from food animals: A review. Food Research International, 45(2), 819-830.
Jeon, H. Y., Seo, K. W., Kim, Y. B., Kim, D. K., Kim, S. W., & Lee, Y. J. (2019). Characteristics of third-generation cephalosporin-resistant Salmonella from retail chicken meat produced by integrated broiler operations. Poultry science, 98(4), 1766-1774.
Lampang, K. N., Chailangkarn, S., & Padungtod, P. (2014). Prevalence and antimicrobial resistance of Salmonella serovars in chicken farm, Chiang Mai and Lamphun province, Northern of Thailand. Veterinary Integrative Sciences, 12(2), 85-93.
Minami, A., Chaicumpa, W., Chongsa-Nguan, M., Samosornsuk, S., Monden, S., Takeshi, K., . . . Kawamoto, K. (2010). Prevalence of foodborne pathogens in open markets and supermarkets in Thailand. Food control, 21(3), 221-226.
Mølbak, K., Gerner-Smidt, P., & Wegener, H. C. (2002). Increasing quinolone resistance in Salmonella enterica serotype Enteritidis. Emerging infectious diseases, 8(5), 514.
Nair, D. V., & Johny, A. K. (2019). Salmonella in Poultry Meat Production Food Safety in Poultry Meat Production (pp. 1-24): Springer.
Neunchat, P., Yangkao, K., Earmsaard, N., Chatuphonprasert, S., Wiwattrakul, J., Manaijsin, J., . . . Kanistranon, K. (2017). SALMONELLA PREVALENCE, ANTIBIOGRAMS AND SEROTYPES FROM CHICKEN MEAT AND EGG IN KHON KAEN CITY, THAILAND. Southeast Asian journal of tropical medicine and public health, 48(6), 1241-1247.
Nidaullah, H., Abirami, N., Shamila-Syuhada, A. K., Chuah, L.-O., Nurul, H., Tan, T. P., . . . Rusul, G. (2017). Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia. Veterinary world, 10(3), 286.
Padungtod, P., & Kaneene, J. B. (2006). Salmonella in food animals and humans in northern Thailand. International journal of food microbiology, 108(3), 346-354.
Phongaran, D., Khang-Air, S., & Angkititrakul, S. (2019). Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand. Veterinary world, 12(8), 1311.
Prevention, C. f. D. C. a. (2020). Current Outbreaks Salmonella Infections (August 27, 2020).
Procura, F., Bueno, D. J., Bruno, S. B., & Rogé, A. D. (2019). Prevalence, antimicrobial resistance profile and comparison of methods for the isolation of Salmonella in chicken liver from Argentina. Food Research International, 119, 541-546.
Quinn, P. J., Markey, B. K., Leonard, F. C., Hartigan, P., Fanning, S., & Fitzpatrick, E. (2011). Veterinary microbiology and microbial disease: John Wiley & Sons.
Shah, A., & Korejo, N. (2012). Antimicrobial resistance profile of Salmonella serovars isolated from chicken meat. J Vet Anim Sci, 2, 40-46.
Sharma, J., Kumar, D., Hussain, S., Pathak, A., Shukla, M., Kumar, V. P., . . . Singh, S. (2019). Prevalence, antimicrobial resistance and virulence genes characterization of nontyphoidal Salmonella isolated from retail chicken meat shops in Northern India. Food control, 102, 104-111.
Soyer, Y., Switt, A. M., Davis, M., Maurer, J., McDonough, P., Schoonmaker-Bopp, D., . . . Gröhn, Y. (2009). Salmonella enterica serotype 4, 5, 12: i:−, an emerging Salmonella serotype that represents multiple distinct clones. Journal of clinical microbiology, 47(11), 3546-3556.
Thai, T. H., Hirai, T., Lan, N. T., & Yamaguchi, R. (2012). Antibiotic resistance profiles of Salmonella serovars isolated from retail pork and chicken meat in North Vietnam. International journal of food microbiology, 156(2), 147-151.
Trongjit, S., Angkititrakul, S., Tuttle, R. E., Poungseree, J., Padungtod, P., & Chuanchuen, R. (2017). Prevalence and antimicrobial resistance in Salmonella enterica isolated from broiler chickens, pigs and meat products in Thailand–Cambodia border provinces. Microbiology and immunology, 61(1), 23-33.
VT Nair, D., Venkitanarayanan, K., & Kollanoor Johny, A. (2018). Antibiotic-resistant Salmonella in the food supply and the potential role of antibiotic alternatives for control. Foods, 7(10), 167.
Wahyono, N., & Utami, M. (2018). A review of the poultry meat production industry for food safety in Indonesia. Paper presented at the Journal of Physics: conference series.
Yan, H., Li, L., Alam, M. J., Shinoda, S., Miyoshi, S.-i., & Shi, L. (2010). Prevalence and antimicrobial resistance of Salmonella in retail foods in northern China. International journal of food microbiology, 143(3), 230-234.
Yang, X., Huang, J., Zhang, Y., Liu, S., Chen, L., Xiao, C., . . . Li, Y. (2020). Prevalence, abundance, serovars and antimicrobial resistance of Salmonella isolated from retail raw poultry meat in China. Science of The Total Environment, 713, 136385.
Zhang, L., Fu, Y., Xiong, Z., Ma, Y., Wei, Y., Qu, X., . . . Liao, M. (2018). Highly prevalent multidrug-resistant Salmonella from chicken and pork meat at retail markets in Guangdong, China. Frontiers in microbiology, 9, 2104.