Histopathological observation and health status of the zebra-snout seahorse Hippocampus barbouri Jordan & Richardson, 1908 in captivity https://doi.org/10.12982/VIS.2021.027
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Abstract
The health status of the zebra-snout seahorse, Hippocampus barbouri in captivity has been required for approval for aquaculture. In this study, we investigated the histopathological appearance of three vital organs including gill, kidney and liver in captive H. barbouri during its juvenile and adult stages, by using histological techniques. In juveniles from stage 14-days (100% prevalence) towards stage 30-days adults (100% prevalence), the gills exhibited intraepithelial edema and necrosis while hepatic tissue showed evidence of intracytoplasmic vacuoles. In addition, histological alteration to renal tissues was observed the degeneration of renal tubules, the presence of melanomacrophage, and the infection of trematode parasites. The parasites were found in stage 30-days adult fish in the kidney (33.3 % prevalence). Taken together, this study highlights the issue of health in captive rearing of H. barbouri, in particular histopathological alterations in gill, liver and kidney tissues, suggesting that aquaculture of this seahorse species requires improved methods and protocols for maintenance and preventing infection.
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References
Alvarez-Pellitero, P., Palenzuela, O., Sitjà-Bobadilla, A., 2007. Histopathology and cellular response in Enteromyxum leei (Myxozoa) infections of Diplodus puntazzo (Teleostei). Parasitol. Int. 57, 110–120.
Anderson, M.J., Cacela, D., Beltman, D., The, S.J., Okihiro, M.S., Hinton, D.E., et al. 2003. Biochemical and toxicopathic biomarkers assessed in smallmouth bass recovered from a polychlorinated biphenyl-contaminated river. Biomark. 8, 371–393.
Aysel, C.K.B., Gulten, K., Ayhan, O., 2008. Sublethal ammonia exposure of nile tilapia (Oreochromis niloticus L.): Effects on gill, liver and kidney histology. Chemosphere. 72, 1355–1358.
Blazer, V.S., 2002. Histopathological assessment of gonadal tissue in wild fishes. ish Physiol. Biochem. 26, 85–101.
Boyd, R.B., De Vries, A.L., Eastman, J.T., Pietra, G.G., 1980. The secondary lamellae of the gills of cold water (high latitude) teleosts. A comparative light and electron microscopic study. Cell Tissue Res. 213, 361–367.
Bury, N.R., Li, J., Flik, G., Lock, R.A.C., Wendelaar-Bonga, S.E., 1998. Cortisol protects against copper induced necrosis and promotes apoptosis in fish gill chloride cells in vitro. Aquat. Toxicol. 40, 193–202.
Caballero, M.J., Lopez-Calero, G., Socorro, J., Roo, F.J., lzquierdo, M.S., Fernandez, A.J., 1999. Combined effect of lipid level and fish meal quality on liver histology of gilthead seabream (Sparus aurata). Aquaculture. 179, 277–290.
de Silva, P.M.C.S., Samayawardhena, L.A., 2002. Effects of chlorpyrifos on reproductive performances of guppy (Poecilia reticulata). Chemoshere. 58, 1293–1299.
Dietrich, D.R., Krieger, H.O., 2009. Histological analysis of endocrine disruptive effects in small laboratory fish. John Wiley and Sons, New Jersey, U.S.A.
Foster, S.J., Vincent, A.C., 2004. Life history and ecology of seahorses: implications for conservation and management. J. Fish Biol. 65, 1–61.
Georgieva, E., Stoyanova, S., Velcheva, I., Yancheva, V., 2014. Histopathological alterations in common carp (Cyprinus carpio L.) gills caused by thiamethoxam. Braz. Arch. Biol. Technol. 57, 991–996.
Greenfield, B.K., The, S.J., Ross, J.R., Hunt, J., Zhang, G., Davis, J.A. et al., 2008. Contaminant concentrations and histopathological effects in Sacramento splittail (Pogonichthys macrolepidotus). Arch. Environ. Contam. Toxicol. 55, 270–281.
Hassaninezhad, L., Safahieh,, A., Salamat, N., Savari, A., Majd, N.E., 2014. Assessment of gill pathological responses in the tropical fish yellowfin seabream of Persian Gulf under mercury exposure. Toxicol. Rep. 1, 621–628.
Hendricks, J.D., Meyers, T.R., Shelton, D.W., 1984. Histological progression of hepatic neoplasia in rainbow trout (Salmo gairdneri). J. Natl. Cancer Inst. 65, 321–336.
Hinton, D.E., Laure´n, D.J., 1990. Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure. In: McCarthy, J.F., Shugart L.R. (Eds.), Biomarkers of Environmental Contamination, pp. 17–57.
Hinton, D.E., Baumann, P.C., Gardner, G.R., Hawkins, W.E., Hendricks, J.D., Murchelano, R.A. et al., 1992. Histopathologic Biomarkers. Biochemical, Physiological, and Histological Markers of Anthropogenic Stress. Biomark. 155–209.
Hutton, J., Dickson, B., 2000. Endangered species, threatened convention: the past, present and future of CITES, the Convention on International Trade in Endangered Species of Wild Fauna and Flora, Earthscan London.
Kamnurdnin, T., 2017. Effects of food on growth and gonadal development of Seahorse, Hippocampus sp (Master degree, Chulalongkorn University.
Meinelt, T., Krüger, R., Pietrock, M., Osten, R., Steinberg, C., 1997. Mercury pollution and macrophage centres in pike (Esox lucius) tissues. Environ. Sci. Pollut. Res. Int. 4, 32–36
Melaa, M., Randi, M.A.F., Ventura, D.F., Carvalho, C.E.V., Pelletier, E., Oliveira, C., Ribeiro, C.A.. 2007. Effects of dietary methyl mercury on liver and kidney histology in the neotropical fish Hoplias malabaricus. Ecotoxicol. Environ. Saf. 68, 426–435.
Organisation for Economic Co-operation and Deveropment (OECD). 2009. OECD guidance document for the diagnosis of endocrine-related histopathology of fish gonads [cited 2018, September 21]. Available from: http://www.oecd.org/dataoecd/33/27/42140701.pdf
Planas, M., Chamorro, A., Quintas P., Vilar. A., 2008. Establishment and maintenance of threatened long-snouted seahorse, Hippocampus guttulatus, broodstock in captivity. Aquaculture 283, 19–28.
Presnell, J.K., Schreibman, M.P., 1997. Humason’s Animal Tissue Techniques. 5th ed. US, Johns Hopkins University Press.
Pritchard, J.B., Bend, J.R., 1984. Mechanisms controlling the renal excretion of xenobiotics in fish: effects of chemical structure. Drug Metab. Rev. 15, 655-671.
Robertson, J.C., Bradley, T.M., 1992. Liver ultrastructure of juvenile Atlantic salmon (Salmo salar). J. Morphol. 211, 41–54.
Robert, R.J., 2012. Fish Pathology. Blackwell Publishing Ltd.
Schrank, C.S., Cormier, S.M., Blazer, V.S., 1997. Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin. J. Great Lakes Res. 23, 119–30.
Senarat, S., Poolprasert, P., Kettratad, J., Boonyoung, B., Jiraungkoorskul, W., Huang, S., Pengsakul, T., Kosiyachinda, P., Sudtongkong, C. 2020a. Histological observation of digestive system of malayan halfbeak, Dermogenys pusilla (Kuhl & van Hasselt, 1823) during juvenile stage from Thailand. Vet. Integr. Sci. 18, 33-41.
Senarat, S., Boonyoung, P., Kettratad, J., Jiraungkoorskul, W., Poolprasert, P., Huang, S., Pengsakul, T., Mongkolchaichana, E., Para, C. 2020b. The identification and distribution of the mucous secreting cells in the integument of the Schaap’s dragonet, Callionymus schaapii, Bleeker, 1852. Vet. Integr. Sci. 18(1): 23-32.
Senarat, S., Kettratad, J., Siriwong, W., Bunsomboonsakul, S., Kenthao, A., Kaneko, G., Sopon, A., Sudtongkong, C., Jiraungkoorskul, W., 2020c. Oogenesis and ovarian health problems in economically important fishes from different habitats potentially affected by pollution in Thailand. Asian Fish. Sci, 33, 274–286.
Senarat, S., Kettratad, J., Gerald Plumley, F., Wangkulangkul, S., Jiraungkoorskul, W., Boonyoung, P., Poolprasert, P., 2019. Pathological microscopy in liver parenchyma of gray-eel catfish, Plotosus canius, from Ang-Sila area, Chonburi Prov-ince, Thailand. Vet. Integr. Sci. 17, 255-261
Senarat, S., Kettretad, J., Poolprasert, P., Tipdomrongpong, S., Plumley, F.G., Jiraungkoorskul, W. 2018a. Health status in wild and captive Rastrelliger brachysoma from Thailand: Histopathology. Songklanakarin J. Sci. Technol. 40, 1090–1097.
Senarat, S., Kettratad, J., Tipdomrongpong, S., Pengsakul, T., Jiraungkoorskul, W., Boonyoung, P., Huang, S., 2018b. Histopathology of kidney and liver in the captive broodstock (Rastrelliger brachysoma) during its juvenile stage. Vet. Integr. Sci. 16, 87-93.
Senarat, S., Kettratad, J., Poolprasert, P., Jiraungkoorskul, W., Yenchum, W., 2015. Histopathological findings of liver and kidney tissues of the yellow mystus, Hemibagrus filamentus (Fang and Chaux, 1949), from the Tapee River, Thailand. Songklanakarin J. Sci. Technol. 37, 1–5.
Sheng, J., Lin, Q., Chen, Q., Gao, Y., Shen L., Lu. J., 2006. Effects of food, temperature and light intensity on the feeding behavior of threespot juveniles eahorses, Hippocampus trimaculatus Leach. Aquaculture 256, 596–607.
Sitja-Bobadilla, A. 2008. Fish immune response to myxozoan parasites. Parasitology. 15, 420–425.
Sola, F., Isaia, J., Masoni, A., 1995. Effects of copper on gill structure and transport function in the rainbow trout, Oncorhynchus mykiss. J Appl. Toxicol. 15, 391–359.
Steinel, N.C., Bolnick, D.I., 2017. Melanomacrophage centers as a histological indicator of immune function in fish and other poikilotherms. Front Immuno. 8, 827.
Suvarna, K.S., Layton, C., Bancroft, J.D., 2013. Bancroft’s Theory and Practice of Histological Techniques. 7th ed. Canada, Elsevier.
Takashima, F., Hibiya, T., 1995. An Atlas of Fish Histology, Normal and Pathological Features, 2nd edition. Kodansha, Tokyo.
The, S.J., Adams, S.M., Hinton, D.E., 1997. Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquatic Toxicol. 37, 51–70.
Tsujii, T., Seno, S., 1990. Melano-macrophage centers in the aglomerular kidney of the sea horse (teleosts): morphologic studies on its formation and possible function. Anat. Rec. 226, 460–470.
Vincent, A.C.J., Foster, S.J., Koldewey H.J., 2011. Conservation and management of seahorses and other Syngnathidae. J. Fish Biol. 78, 1681–1724.
Wung, L.Y., Christianus, A., Zakaria, M.H., Min, C.C., Worachananant, S. 2020. Effect of cultured artemia on growth and survival of juvenile Hippocampus barbouri. J. Fush. Envieron. 44, 40-49.
Wilson, J.M., Bunte, R.M., Carty, A.J., 2009. Evaluation of rapid cooling and tricaine methanesulfonate (MS 222) as methods of euthanasia in zebrafish (Danio rerio). J. Am. Assoc. Lab. Anim. Sci. 48, 785–789.
Wilson, M.J., Vincent, A.C.J., 2000. Preliminary success in closing the life cycle of exploited seahorse species, Hippocampus spp., in captivity. Aquarium Sci. and Conserv. 2, 179–196.
Yenchum, W., 2010. Histological effects of carbofuran on guppy Poecilia reticulata Peters (Doctoral thesis, Chulalongkorn University.
Zilberg, D., Munday, B.L., 2002. Pathology of experimental amoebic gill disease in Atlantic salmon, Salmo salar L., and the effect of pre-maintenance of fish in sea water on the infection. J. Fish Dis. 23, 401–407.