Spermatozoon of the wild scalloped perchlet, Ambassis nalua (Hamilton, 1822): Ultrastructure and morphometric analysis https://doi.org/10.12982/VIS.2022.016
Article Sidebar
Main Article Content
Abstract
The description of sperm morphology is fundamental in the reproductive biology of fishes, but this information is limited in the family Ambassidae. Our report hence focused on the ultrastructure and morphometric analysis of spermatozoa in a pelagic fish Ambassis nalua. All fish (n = 75) were obtained during January and March 2017 from the Estuarine Pranburi River, Thailand. The standard length of fish used in this study was 3.4 ± 0.12 cm (mean ± standard deviation). All specimens were considered mature based on the abundance of spermatozoa in the testis. The testicular organs were collected and observed using standard histology and transmission electron microscopy (TEM). Ultrastructural observation associated with morphometric analysis showed that spermatozoa are structurally long cells of approximately 51.17 ± 4.54 µm total length, composed of a head, a midpiece and a tail. The head had no acrosome, and the granular structure of condensed chromatin was observed within the ovoid nucleus. The midpiece consisted of a short cylindrical region with the length of 1.29 ± 0.87 µm in diameter, having the centriolar complex organization and eight mitochondria (approx. 0.32 ± 0.02 µm each). The uniflagellar tail was clearly identified with a classical 9+2 arrangements of microtubules. Based on these characteristics, the spermatozoon of wild scalloped perchlet are considered as uniflagellate anacrosomal aquqsperm. The morphological features, including the number of mitochondria, may be used for further cryopreservation and in the evolutionary biology of this species.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.
References
Abascal, F.J., Medina, A., Megina, C., Calzada, A., 2002. Ultrastructure of Thunnus thynnus and Euthynnus allettevatus spermatozoa. J. Fish biol., 60(1), 147 –153.
Alavi, S.M.H., Psenicka, M., Rodina, M., Policar, T., Linhart, O., 2008. Changes of sperm morphology, volume, density and motility and seminal plasma composition in Barbus barbus (Teleostei: Cyprinidae) during the reproductive season. Aquat. Living Resour., 21(1), 75–80.
Arianti, N.D., Rahardjo, M.GF., Zahid, A. 2017. Oocyte development of scalloped perchlet, Ambassis nalua (Hamilton 1822). Indon. J. Ichthyo., 17, 115–123.
Baccetti, B., Burrini, A. G., Gibertini, G., Mazzini, M., Zerunian, S., 1984. Fish germinal cells. I. Comparative spermatology of seven cyprinid species. Gamete Res. 10(4), 373–396.
Biagi, F., Piras, F., Farina, V., Zedda, M., Mura, E., Floris, A., Franzoi, P., Fausto, A.M. Taddei, A.R., Carcupino, M., 2016. Testis structure, spermatogenesis and sperm morphology in pipefishes of the genus Syngnathus. Acta Zool., 97, 90–101.
Billard R.B., 1970. Ultrastructure comparée de spermatozoides de quelques poissons téléostéens, New York Academic Press, pp. 71–79.
Billard, R., Cosson, J., Linhart, O., 2000. Changes in the flagellum morphology of intact and frozen/thawed Siberian sturgeon Acipenser baerii (Brandt) sperm during motility Aquac. Res., 31, 283– 287
Biagi, F., Piras, F., Farina, V., Zedda, M., Mura, E., Floris, A., Franzoi, P., Fausto, A.M., Taddei, A.R., Carcupino, M., 2016. Testis structure, spermatogenesis and sperm morphology in pipefishes of the genus Syngnathus. Acta Zool., 97(1), 90–101.
Burns, J.R., Weitzman, S.H., 2005. Insemination in Ostariophysan Fishes. In: Uribe, M.C., Grier, H.J. (Eds.), Viviparous Fishes, Homestead, New Life Publications, pp. 105–132.
Cosson, J., Groison, A.L., Suquet, M., Fauvel, C., Dreanno, C., Billard, R., 2008. Marine fish spermatozoa: racing ephemeral swimmers. Reproduc., 136, 277–294.
Chung, E.Y., 2008. Ultrastructure of germ cells, the Leydig cells, and Sertoli cells during spermatogenesis in Boleophthalmus pectinirostris (Teleostei, Perciformes, Gobiidae). Tissue Cell., 40(3), 195–205.
Cosson, J., Billard, R., Cibert, C., Dreanno, C., Suquet, M., 1999. Ionic factors regulating the motility of fish sperm. The male gamete: from basic science to clinical applications. 161–186.
Fahmy, A.F., El-Gharabawy, M.M., Assem, S.S., 2007. Steroid hormone in serum of male mugil cephalus from lake quaron in relation to ultrastructure of steroidogenic secreting tissue. Egypt. J. Aquat. Res., 33(3), 156–178.
Geiger, N., 1955. Electron microscopy studies on salmonid spermatozoa. Revue Suisse Zool. 62, 325-334.
Ginzburg, A.S., 1968. Fertilization in fishes and the problem of polyspermy. Academy of Sciences of the USSR, Institute of Developmental Biology. Translated from Russian. In: Blake, Z., Golek, B. (Eds.), Israel Program for Scientific Translations, Jerusalem, 1972.
Grier, H.J., 1981. Cellular organization of the testis and spermatogenesis in fishes. Am. Zool., 21(2), 345–357.
Hara, M., Okiyama, M., 1998. An ultrastructural review on the spermatozoa of Japanese fishes. Bull. Ocean Res. Inst. Univ. Tokyo., 33, 1–138.
Jamieson, B.G., 2009. Reproductive biology and phylogeny of fishes (agnathans and bony fishes): phylogeny, reproductive system, viviparity, spermatozoa. CRC press.
Jamieson, B.G., Leung, L.P., 1991. Fish evolution and systematics: evidence from spermatozoa: with a survey of lophophorate, echinoderm and protochordate sperm and an account of gamete cryopreservation. Cambridge University Press.
Jamieson, B.G.M., Grier, H.J., 1993. Influences of phylogenetic position and fertilization biology on spermatozoal ultrastructure exemplified by exocoetoid and poeciliid fish. Hydrobiologia., 271(1), 11–25.
Lahnsteiner, F., Berger, B., Weismann, T., Patzner, R., 1995. Fine structure and motility of spermatozoa and composition of the seminal plasma in the perch. J. Fish Biol., 47(3), 492–508.
Malo, A.F., Gomendio, M., Garde, J., Lang-Lenton, B., Soler, A. J., Roldan, E.R., 2006. Sperm design and sperm function. Biol. lett., 2(2), 246–249.
Medina, A., Megina, C., Abascal, E. J., Calzada, A., 2000. The spermatozoon morphology of Sozeu seneguzensis (Kaup, 1858) (Teleostei, Pleuronectiformes). J. Submicrosc. Cytol. Pathol., 32(4), 645–650.
Maricchiolo, G., Genovese, L., Laurà, R., Micale, V., Muglia, U., 2004. Fine structure of spermatozoa in the common pandora (Pagellus erythrinus Linnaeus, 1758) (Perciformes, Sparidae). Histology and histopathology. Histol. Histopath., 19(4), 1237–1440.
Maricchiolo, G., Laurà, R., Genovese, L., Guerrera, M. C., Micale, V., Muglia, U., 2010. Fine structure of spermatozoa in the blackspot sea bream Pagellus bogaraveo (Brünnich, 1768) with some considerations about the centriolar complex. Tissue Cell., 42(2), 88–96.
Mattei, X., 1991. Spermatozoon ultrastructure and its systematic implications in fishes. Can. J. Zool., 69(12), 3038–3055.
Mattei, C., 1970. Spermiogeneses comparee des Poissons. In: Baccetti, B. (Ed.), Comparative Spermatology. Academic Press, New York, pp. 57–69.
Medina, A., Megina, C., Abascal, F.J., Calzada, A., 2000. The spermatozoon morphology of solea senegalensis (Kaup, 1858) (Teleostei, Pleuronectiformes). J. Submicrosc. Cytol. Pathol., 32, 645–650.
Poirier, G. R., Nicholson, N., 1982. Fine structure of the testicular spermatozoa from the channel catfish, Ictalurus punctatus. J. Ultrastruct. Res., 80(1), 104–110.
Presnell, J.K., Schreibman, M.P., Humason, G.L., 1997. Humason's animal tissue techniques. Johns Hopkins University Press, Baltimore.
Quagio-Grassiotto, I., Spadella, M. A., Carvalho, M. D., Oliveira, C. 2005. Comparative description and discussion of spermiogenesis and spermatozoal ultrastructure in some species of Heptapteridae and Pseudopimelodidae (Teleostei: Siluriformes). Neotrop. Ichthyol., 3, 401–410.
Quagio-Grassiotto, I., Ortiz, R.J., Pérez, M.S., Oliveira, C., 2011. Sperm of Doradidae (Teleostei: Siluriformes). Tissue Cell., 43(1), 8–23.
Retzius, G., 1906. Die spermien der marsupialier. Biol. Untersuch., 13, 75–86.
Reznick, D., Ghalambor, C., Nunney, L., 2002, The evolution of senescence in fish. Mech. Ageing Dev., 123, 773–789.
Rouse, G.W., Jamieson, B.G.M., 1987. An ultrastructural study of the spermatozoa of the polychaetes Eurythoe complanata (Amphinomidae), Clymenella sp. and Micromaldane sp. (Maldanidae), with definition of sperm types in relation to reproductive biology. J Submicrosc. Cytol. Pathol., 19: 573–584.
Senarat, S., Kettretad, J., Jiraungkoorskul, W., 2018a. Testicular structure and spermatogenesis of short mackerel, Rastrelliger brachysoma (Bleeker, 1851) in The Upper Gulf of Thailand. Asia Pac. J. Mol. Biol., 26(2), 30–43.
Senarat, S., Kettratad, J., Tipdomrongpong, S., Pengsakul, T., Jiraungkoorskul, W., Boonyoung, P., Huang, S. 2018b. Histopathology of kidney and liver in the captive broodstock (Rastrelliger brachysoma) during its juvenile stage. Vet. Integr. Sci. 16, 87–93.
Senarat, S., Kettratad, J., Plumley, F.G., Wangkulangkul, S., Jiraungkoorskul, W., Boonyoung, P., Poolprasert, P. 2019. Pathological microscopy in liver parenchyma of gray-eel catfish, Plotosus canius, from the Ang-Sila area, Chonburi Province, Thailand. Vet. Integr. Sci. 17, 255–261.
Sukkhee, N., Mitparian, T., Kanjanarakha, T., Senarat, S., Jiraungkoorskul, W., Kangwanrangsan, N., Kaneko, G., Kettratad, J., 2021.
Spermatogenic ultrastructure of the grunting toadfish Allenbatrachus grunniens (Batrachoididae). J. Ichthyol., 61, 467–475
Suvarna, S.K., Layton, C., 2013. Bancroft’s theory and practice of histological techniques. 7th. Churchill Livingstone Elsevier.
Vergílio, C.D.S., Moreira, R.V., de Carvalho, C.E., de Melo, E.J., 2013. Characterization of mature testis and sperm morphology of Gymnotus carapo (Gymnotidae, Teleostei) from the southeast of Brazil. Acta Zool., 94(3), 364–370.
Wilson, J.M., Bunte, R.M., Carty, A.J., 2009. Evaluation of rapid cooling and tricaine methanesulfonate (MS222) as methods of euthanasia in zebrafish (Danio rerio). J. Am. Assoc. Lab. Anim. Sci., 48(6), 785–789.