LAMP assay is an auxiliary tool for rapid and sensitive pathogen diagnosis in veterinary areas https://doi.org/10.12982/VIS.2022.019

Main Article Content

Kittisak Buddhachat

Abstract

Molecular technique for nucleic acid amplification has become more advanced, especially isothermal nucleic acid amplification which can amplify DNA or RNA molecules at constant temperatures. This allows us to be independent of sophisticated equipment such as thermal cyclers or realtime-PCR. The feasibility of adopting molecular techniques for pathogen detection at point-of-care are approaching practical use. At present, there are various isothermal nucleic acid amplification technologies, including rolling cycle amplification (RCA), helicase-dependent amplification (HDA), recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP). LAMP is one of the most popular methods used for pathogen diagnosis in animals due to several readout platforms such as turbidity, fluorescence, lateral flow dipstick (LFD) and color change visualizable by naked eye. In addition, LAMP can be integrated with CRISPR-cas which in turn, augments pathogen detection at ultra sensitivity. This letter shows the implementation of LAMP for pathogen detection in veterinary areas with different readouts as well as a new perspective for LAMP adaptation.

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How to Cite
Buddhachat, K. . (2022). LAMP assay is an auxiliary tool for rapid and sensitive pathogen diagnosis in veterinary areas: https://doi.org/10.12982/VIS.2022.019. Veterinary Integrative Sciences, 20(2), 247–251. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/256100
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References

Böger, B., Fachi, M.M., Vilhena, R.O., Cobre, A.F., Tonin, F.S., Pontarolo, R., 2021. Systematic review with meta-analysis of the accuracy of diagnostic tests for COVID-19. American Journal of infection control, 49(1), 21-29.

Buddhachat, K., Ritbumrung, O., Sripairoj, N., Inthima, P., Ratanasut, K., Boonsrangsom, T. and Sujipuli, K., 2021. One-step colorimetric LAMP (cLAMP) assay for visual detection of Xanthomonas oryzae pv. oryzae in rice. Crop Protection, p.105809.

Chen, J.S., Ma, E., Harrington, L.B., Da Costa, M., Tian, X., Palefsky, J.M. and Doudna, J.A., 2018. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science, 360(6387), pp.436-439.

Craw, P. and Balachandran, W., 2012. Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review. Lab on a Chip, 12(14), pp.2469-2486.

Destoumieux-Garzón, D., Mavingui, P., Boetsch, G., Boissier, J., Darriet, F., Duboz, P., Fritsch, C., Giraudoux, P., Le Roux, F., Morand, S. and Paillard, C., 2018. The one health concept: 10 years old and a long road ahead. Frontiers in veterinary science, 5, p.14.

Dokphut, A., Boonpornprasert, P., Songkasupa, T. and Tangdee, S., 2021. Development of a loop-mediated isothermal amplification assay for rapid detection of African swine fever. Veterinary Integrative Sciences, 19(1), pp.87-100.

Goto, M., Honda, E., Ogura, A., Nomoto, A. and Hanaki, K.I., 2009. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques, 46(3), pp.167-172.

Hsieh, K., Mage, P.L., Csordas, A.T., Eisenstein, M. and Soh, H.T., 2014. Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Chemical communications, 50(28), pp.3747-3749.

Li, S.Y., Cheng, Q.X., Wang, J.M., Li, X.Y., Zhang, Z.L., Gao, S., Cao, R.B., Zhao, G.P. and Wang, J., 2018. CRISPR-Cas12a-assisted nucleic acid detection. Cell discovery, 4(1), pp.1-4.

Mukama, O., Yuan, T., He, Z., Li, Z., de Dieu Habimana, J., Hussain, M., Li, W., Yi, Z., Liang, Q. and Zeng, L., 2020. A high fidelity CRISPR/Cas12a based lateral flow biosensor for the detection of HPV16 and HPV18. Sensors and Actuators B: Chemical, 316, p.128119.

Notomi, T., Mori, Y., Tomita, N. and Kanda, H., 2015. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. Journal of microbiology, 53(1), pp.1-5.

Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. and Hase, T., 2000. Loop-mediated isothermal amplification of DNA. Nucleic acids research, 28(12), pp.e63-e63.

Pang, B., Yao, S., Xu, K., Wang, J., Song, X., Mu, Y., Zhao, C. and Li, J., 2019. A novel visual-mixed-dye for LAMP and its application in the detection of foodborne pathogens. Analytical biochemistry, 574, pp.1-6.

Pikulkaew, S. and Potibut, P., 2021. Establishment of loop-mediated isothermal amplification assay for detection of parasitic ciliate Ichthyophthirius multifiliis in cyprinid fish. Veterinary Integrative Sciences, 19(3), pp.379-390.

Posthuma-Trumpie, G.A., Korf, J. and van Amerongen, A., 2009. Lateral flow (immuno) assay: its strengths, weaknesses, opportunities and threats. A literature survey. Analytical and bioanalytical chemistry, 393(2), pp.569-582.

Quyen, T.L., Ngo, T.A., Bang, D.D., Madsen, M. and Wolff, A., 2019. Classification of multiple DNA dyes based on inhibition effects on real-time loop-mediated isothermal amplification (LAMP): prospect for point of care setting. Frontiers in microbiology, 10, p.2234.

Tanner, N.A., Zhang, Y. and Evans Jr, T.C., 2015. Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. Biotechniques, 58(2), pp.59-68.

Vandenberg, O., Martiny, D., Rochas, O., van Belkum, A. and Kozlakidis, Z., 2021. Considerations for diagnostic COVID-19 tests. Nature Reviews Microbiology, 19(3), pp.171-183.

Yang, Y., Li, Q., Wang, S., Chen, X. and Du, A., 2016. Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick. Veterinary parasitology, 219, pp.71-76.

Zhang, H., Xu, Y., Fohlerova, Z., Chang, H., Iliescu, C. and Neuzil, P., 2019. LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification. TrAC Trends in Analytical Chemistry, 113, pp.44-53.

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