Main Article Content
The study was conducted to determine the effects of different factors on increased pregnancy rate of Bangladeshi ewes through LAP-AI. A total of 24 indigenous ewes and 4 rams were selected. Frozen semen with a concentration of 100×106 spermatozoa/ 0.25 ml straws were produced. The selected ewes were synchronized using two doses of PGF2α @ 175µg per ewe at 9 days intervals intramuscularly. Estrus was confirmed by the vasectomized ram. The LAP-AI was performed at two different times from the onset of estrus, two angles of placement on the laparoscopic cradle, and two different puncture sites. Pregnancy was confirmed by ultrasonography on days 45-50 following insemination. The pregnancy rate of ewes 2.5-4 years of age and 16-20 kg bodyweight was higher (75% vs. 41.67%) than ewes 1-2 years and 9-13 kg. However, this was not significant (p>0.05). The pregnancy rate of ewes having BCS >3 was significantly higher (p<0.05) (78.57% vs. 30%) than BCS 2-2.5. The 20-24 hours insemination time from the onset of estrus yielded a significantly higher (p<0.05) (75% vs. 25%) pregnancy rate than 12-16 hours. The pregnancy rate was higher (66.67% vs. 50%) at 45° than 30°angle of placement of ewes on the laparoscopic cradle. However, there was no significant difference (p>0.05). The puncture point, 4-5 cm away from the udder resulted from a significantly higher pregnancy rate (p<0.05) (83.33% vs. 33.33%) than 7-10 cm. The present study indicates that the pregnancy rates following LAP-AI using frozen semen could be increased if the significant factors are considered
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publishing an article with open access in Veterinary Integrative Sciences leaves the copyright with the author. The article is published under the Creative Commons Attribution License 4.0 (CC-BY 4.0), which allows users to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited.
Alejandro, E.G., Jimena, F., Maria, M.B., Maria, V.S., Marcela, I.C., 2019. Technical recommendation for artificial insemination in sheep. Anim. Reprod. 16(4), 803-809.
Aliyari, D., Moeini, M.M., Shahir, M.H., Sirjani, M.A., 2012. Effect of body condition score, live weight and age on reproductive performance of Afshari Ewes. Asian J. Anim. Vet. Adv. 7, 904-909.
Anel, L., Kaabi, M., Abroug, B., Alvarez, M., Anel, E., Boixo, J.C., De la Fuente, L.F., De paz, P., 2005. Factors influencing the success of vaginal and laparoscopic artificial insemination in Churra ewes: a field assay. Theriogenology. 63 (4), 1235-1247.
Azizunnesa, 2016. Preservation of indigenous ram semen. PhD Thesis, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Bari, F.Y., Haresign, W., 1998. Embryo recovery, fertilization rates and embryo quality and survival rates in the superovulated ewes following natural service and/or laparoscopic intrauterine insemination. The Ban.Vet. 15, 1-6.
Bhuiyan AKFH 2006: Livestock genetic resources in Bangladesh: Preservation and Management. International conference on livestock services, Chinese Academy of Agricultural Science (CAAS), Beijing, China, April 16-20.
Dovenski, T., Trojacanec, P., Petkov, V., Popovska-Percinic, F., Kocoski, L., Grizelj, J., 2012. Laparoscopy-promising tool for improvement of reproductive efficiency of small ruminants. Mac. Vet. Rev. 35 (1), 5–11.
Duran, B.J., 2018. Investigation into factors potentially influencing the success of laparoscopic artificial insemination in sheep. Iowa State University Capstones, Theses and Dissertations.
El-Badry, D.A., Aml, Z.L., and Mona, H.S., 2014. Studies on laparoscopic intrauterine insemination of barki ewes (using different insemination doses) as compared with cervical insemination. Assiut Vet. Med. J. 60, 172-178.
Fukui, Y., Kohno, H., Okabe, K., Katsuki, S., Yoshizawa, M., Togari, T., and Watanabe, H., 2010. Factors affecting the fertility of ewes after intrauterine insemination with frozen-thawed semen during the non-breeding season. J. Reprod. Dev. 56(4), 460-466.
Gonzalez, R.E., Labuonora, D., & Russel, A.J.F., 1997. The effects of ewe live weight and body condition score around mating on production from four sheep breeds in extensive grazing systems in Uruguay. Anim. Sci. 64(1), 139-145.
Gourley, D.D., and Riese, R.L., 1990. Laparoscopic Artificial Insemination in Sheep. Vet. Clin. North Am. Food Anim. Pract. 6(3), 615-633.
Haresign, W., 1990. Controlling reproduction in sheep. In: New Development in Sheep Production. BSAP Occasional Publication 14, 23 – 37.
Jha, P.K., Alam, M.G.S., Bari, F.Y., 2020. Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes. Vet. Sci.: Res. Rev. 6(2), 143-149.
Jha, P.K., Alam, M.G.S., Mansur, M.A.A., Naher, N., Islam, T., Bhuiyan, M.U., Bari, F.Y., 2019. Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques. Cryobiol. 89, 35-41.
Kaabi, M., Alvarez, M., Anel, E., Chamorro, C.A., Boixo, J.C., de Paz, P., and Anel, L., 2006. Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix: a postmortem study. Theriogenology. 66, 1876–1883.
Kershaw, C.M., Khalid, M., McGowan, M.R., Ingram, K., Leethongdee, S., Wax, G., Scaramuzzi, R.J., 2005. The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminating pipette into the uterine lumen. Theriogenology. 64 (5), 1225-1235.
Kumar, D., Naqvi, S.M.K., 2014. Effect of time and depth of insemination on Bharat Merino sheep inseminated transcervical with frozen-thawed semen. J. Anim. Sci. Technol. 56, 1-6.
Maxwell, W.M.C., and Hewitt, L.J., 1986. A comparison of vaginal, cervical and intrauterine insemination in sheep. J. Agric. Sci. 106 (1), 191-193.
Maxwell, W.M.C., Butler, L.G. and Wilson, H.R., 1984. Short note. Intra-uterine insemination of ewes with frozen semen. J. Agric. Sci. Camb. 102, 233-235.
Moses, D., Martinez, A.G., Iorio, G., Valcarcel, A., Ham, A., Pessi, H., Castanon, R., Marcia, A., de las Heras, M.A., 1997. A large-scale program in laparoscopic intrauterine insemination with frozen-thawed semen in Australian merino sheep in Argentine Patagonia. Theriogenology. 48(4), 651-657.
Mukherjee TK 2000: Final Consultancy Report on Goat and Sheep Production. Agriculture Research Management Project (BLRI Part) IDA, Credit No. 2815 BD, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh
Naher, N., Juyena, N.S., Jha, P.K., Talukder, M.R.I., Alam, M.G.S., and Bari, F.Y., 2017. Factors influencing the pregnancy rate in indigenous ewes following AI using frozen semen. The Ban Vet. 33(2), 33 – 38.
Oliveira Filho, B.D., Toniollo, G.H., Oliveira, A.F., Viu, M.A., Ferraz, H.T., Lopes, D.T., Gambarini, M.L., 2010. The effect of offering an energy and protein supplement to grazing canchim beef cow either postpartum or both pre and post-partum on lipid blood metabolites and folliculogenesis. Anim. Reprod. Sci. 121, 39-45.
Robinson, J.J., Wallace, J.M., Aitken, R.P., 1989. Fertilization and ovum recovery rates in superovulated ewes following cervical insemination or laparoscopic intrauterine at different times after progestagen withdrawal in one or both uterine horns. J. Reprod. Fertil. 87, 771-782.
Russel, A.J., Doney, F.J.M., Gunn, R.G., 1969. Subjective assessment of fat in live sheep. J. Agric. Sci. Cambridge 72, 451–454.
Sathe, S.R., 2018. Laparoscopic Artificial Insemination Technique in Small Ruminants-A Procedure Review. Front. Vet. Sci. 5, 266.