The effect of different media and temperature conditions for Salmonella bacteriophage preservation

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Le Nguyen Nam Phuong
Luu Huynh Anh
Pham Khanh Nguyen Huan
Huynh Tan Loc
Chau Thi Huyen Trang
Trinh Thi Hong Mo
Nguyen Hong Xuan
Nguyen Trong Ngu


This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended.


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Nam Phuong, L. N. ., Huynh Anh, L. ., Khanh Nguyen Huan, P. ., Tan Loc, H. ., Thi Huyen Trang, C. ., Thi Hong Mo, T. ., Hong Xuan, N. ., & Trong Ngu, N. . (2022). The effect of different media and temperature conditions for Salmonella bacteriophage preservation: Veterinary Integrative Sciences, 20(2), 489–496. Retrieved from
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