Epidemiology and Antimicrobial Resistance of Salmonella Isolated from Racehorses and Horsemen in Northeastern Thailand https://doi.org/10.12982/VIS.2022.037

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Ruxpon Dejkong
Suchat Wattanachai
Patchara Phuektes
Suphannika Putthachalee
Sunpetch Angkititrakul

Abstract

Salmonella is one of the major causes of foodborne diseases in humans.  These bacteria can colonize within the gastrointestinal tracts of both humans and animals, and there have been reports of incidences of Salmonella in horses. This study aimed to investigate the prevalence, serotypes, and antimicrobial resistance of Salmonella isolates from racehorses and horsemen, as well as to explore the possible transmission between horses and humans. Fecal samples from racehorses (247 samples) and horsemen (33 samples) were collected from horse farms located in 3 provinces of upper Northeastern Thailand between March and August 2019. Salmonella was isolated and identified. Broth microdilution was used to determine the minimal inhibitory concentrations (MICs) of the antimicrobial agents for antimicrobial. Salmonella isolates were detected in 4.86% (12/247) of racehorses and 3.03% (1/33) of horsemen. The most commonly found serotypes in the isolates obtained from the racehorses were Abony (25%) and Iganda (16.67%). Only the Tumodi II serotype was found in one horseman. Salmonella isolates collected from the racehorses had been the most resistant to streptomycin  (66.67%), while the isolate from a horsemen had been resistant to ampicillin, streptomycin, oxytetracycline, and to tetracycline. Although Salmonella transmission between racehorses and horsemen was not found to be obviously present in this study, the appropriate use of antimicrobials and hygienic procedures are still necessary in order to prevent antimicrobial resistance and the transmission of drug-resistant Salmonella between horses and humans

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How to Cite
Dejkong, R. ., Wattanachai, S. ., Phuektes, P. ., Putthachalee, S. ., & Angkititrakul, S. . (2022). Epidemiology and Antimicrobial Resistance of Salmonella Isolated from Racehorses and Horsemen in Northeastern Thailand : https://doi.org/10.12982/VIS.2022.037. Veterinary Integrative Sciences, 20(2), 497–506. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/258252
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Research Articles

References

Alinovi, C.A., Ward, M.P., Couetil, L.L., and Wu, C.C. 2003a. Detection of Salmonella organisms and assessment of a protocol for removal of contamination in horse stalls at a veterinary teaching hospital. JAVMA. 223(11): 1640-1644.

Alinovi, C.A., Ward, M.P., Couetil, L.L., and Wu, C.C. 2003b. Risk factors for fecal shedding of Salmonella from horses in a veterinary teaching hospital. Preventive Veterinary Medicine. 60: 307-317.

Angkititrakul, S., Chomvarin, C., Chaita, T., Kanistanon, K., and Waethewutajarn, S. 2005. Epidemiology of antimicrobial resistance in Salmonella isolated from pork, chicken meat and humans in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health. 36(6): 1510-1515.

Anzai, T., Kuwamoto, Y., Wada, R., Akiba, M., Ohya, T., and Sameshima, T. 2003. Virulence and other biological properties of Salmonella Typhimurium isolated from foals in Japan from 1981 until 1996. Journal of Equine Science. 14(3): 87-92.

Bangtrakulnonth, A., Pornreongwong, S., Pulsrikarn, C., Sawanpanyalert, P., Hendriksen, R.S., Wong, D.M.A.L.F., and Aarestrup, F.M. 2004. Salmonella serovars from humans and other sources in Thailand, 1993–2002. Emerging Infectious Disease. 10(1): 131-136.

Carter, J. D., Hird, D. W., Farver, T. B., & Hjerpe, C. A. (1986). Salmonellosis in hospitalized horses: seasonality and case fatality rates. Journal of the American Veterinary Medical Association, 188(2), 163-167.

Centers of Disease Control and Prevention (CDC). 2019. Antibiotic Resistance Threats in the United States, 2019. Atlanta (GA): U.S. Department of Health and Human Services. 140 p.

Chapman, A.M. 2006. Characterizing Salmonella fecal shedding among racehorses in Louisiana [Thesis]. Baton Rouge (LA): Louisiana State University. 60 p.

Clinical and Laboratory Standards Institute (CLSI). 2017. Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. Pennsylvania: Clinical and Laboratory Standards Institute.

European Food Safety Authority (EFSA). 2011. Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals. European Food Safety Authority Journal. 9(8): 1-95.

Fielding, C.L., Meier, C.A., Magdesian, K.G., and Pusterla, N. 2013. Salmonella spp. fecal shedding detected by real-time PCR in competing endurance horses. Veterinary Journal. 197: 876–877.

Finn, S., Condell, O., McClure, P., Amezquita A., and Fanning, F. 2013. Mechanisms of survival, responses, and sources of Salmonella in low-moisture environments. Frontiers in Microbiology. 4: 1-15.

Hernandez, J.A., Long, M.T., Traub-Dargatz, J.L., and Besser, T.E. 2014. Salmonellosis. In: Sellon, D.C., and Long, M.T., editors. Equine infectious diseases. 2nd ed. China: Saunders Elsevier. p. 321-332.

House, J.K., Mainar-Jaime, R.C., Smith, B.P., House, A.M., and Kamiya, D.Y., 1999. Risk factors for nosocomial Salmonella infection among hospitalized horses. Journal of the American Veterinary Medical Association. 214(10): 1511-1516.

International Organization for Standardization (ISO). 2019. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices - Part 1: Broth micro-dilution reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. 2nd ed. Geneva: International Organization for Standardization.

International Organization for Standardization (ISO). 2017. Microbiology of the food chain - horizontal method for the detection, enumeration and serotyping of Salmonella. – Part 1: Detection of Salmonella spp. 1st ed. Geneva: International Organization for Standardization.

Janiyayotin, T., Raekngam, S., Chunsuthiwat, S., and Thavonnan, J. 1999. Guide of prevention and control diarrhea. Thailand: Agricultural Cooperative Federation. 187 p.

Jay-Russell, M.T., Madigan, J.E., Bengson, Y., Madigan, S., Hake, A.F., Foley, J.E., and Byrne, B.A. 2014. Salmonella Oranienburg isolated from horses, wild turkeys and an edible home garden fertilized with raw horse manure. Zoonoses and Public Health. 61: 64-71.

Karon, A.E., Archer, J.R., Sotir, M.J., Monson, T.A., and Kazmierczak, J.J. 2007. Human multidrug-resistant Salmonella Newport infections, Wisconsin, 2003–2005. Emerging Infectious Diseases. 13(11): 1777-1780.

Kim, L.M., Morley P.S., Traub-Dargatz, J.L., Salman, M.D., and Gentry-Weeks C. 2001. Factors associated with Salmonella shedding among equine colic patients at a veterinary teaching hospital. JAVMA. 218(5): 740-748.

McCain, C.S., and Powell, K.C. 1990. Asymptomatic salmonellosis in healthy adult horses. Journal of Veterinary Diagnostic Investigation. 2: 236-237.

National Antimicrobial Resistant Surveillance Center, Thailand (NARST). 2020. Antimicrobial Resistance 2000-2020(6M). National Antimicrobial Resistant Surveillance Center, Thailand.

Sringam, P., Tungtanatanich, P., Suwannachot, P., Suksawat, F., Khang-Air, S., and Angkititrakul, S. 2016. Molecular epidemiology and antibiogram of Salmonella isolates from humans, swine and pork. Southeast Asian Journal of Tropical Medicine and Public Health. 48(5): 1017-1028.

Suchatlikitwong, P., and Pancharoen, C. 2019. Drug-Resistant Bacteria in Acute Diarrhea. In: Jantarabenjakul, W., Anugulruengkitt, S., Puthanakit T., and Pancharoen, C, editors. Drug-Resistant Organisms in Pediatrics: Diagnosis and Treatment. Bangkok: Chulalongkorn University. p. 119-134.

Traub-Dargatz, J.L., Garber, L.P., Fedorka-Cray, P.J., Ladely, S., and Ferris, K.E. 2000. Fecal shedding of Salmonella spp. by horses in the United States during 1998 and 1999 and detection of Salmonella spp. in grain and concentrate sources on equine operations. Journal of the American Veterinary Medical Association. 217(2): 226-230.

Vaeteewootacharn, K., Sutra, S., Vaeteewootacharn, S., Sithigon, D., Jamjane, O., Chomvarin, C., Hahnvajanawong, C., Thongskulpanich, N., and Thaewnon-giew, K., 2005. Salmonellosis and the food chain in Khon Kaen, northeastern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health. 36(1): 123-129.

Yang, W., Phongaran, D., Prasertsee, T., Fang, R., Phuektes, P., and Angkititrakul, S. 2018. Molecular epidemiology and antimicrobial resistance of Salmonella spp. Isolated from broilers and pigs at slaughterhouses in Thailand and China. Thai Journal of Veterinary Medicine. 48(3): 393-401.