Anthelmintic efficacy of mango seed extract on porcine gastrointestinal nematodes

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Sentina Rhea T. Reyno
Loveille Jun A. Gonzaga
Elsa A. Gonzaga


The usual control for gastrointestinal parasites is the use of commercial anthelmintics. However, parasites are becoming more resistant due to the frequent and inappropriate use of these anthelmintics. As a result, alternatives for these anthelmintics are becoming increasingly widespread for deworming livestock, particularly those that come from natural sources. Thus, this study aimed to evaluate the efficacy of mango (var. Carabao) seed extract in reducing the egg per gram counts of common gastrointestinal nematodes in pigs and compare the efficacy with that of levamisole. Experimental animals were naturally infected pigs with Trichuris spp. and Strongyloides spp. being the most predominant. The animals were administered orally with a single dose of the mango seed extract at concentrations of 600, 700, and 800 mg per kg bodyweight. The extract, regardless of concentration, was able to decrease the epg counts. Within 14 days post-treatment, there was no significant difference in the efficacies in the administration of 800 mg extract per kg bodyweight and levamisole. This comparable efficacy was sustained until 28 days post-treatment. This significant in vivo anthelmintic activity may be attributed to the tannins and flavonoids present in the extract. These results indicate that the mango seed
extract is effective in controlling and reducing the gastrointestinal nematodes in pigs and may have the potential to be further developed as an anthelmintic.

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Rhea T. Reyno, S. ., Jun A. Gonzaga, L., & A. Gonzaga, E. . (2022). Anthelmintic efficacy of mango seed extract on porcine gastrointestinal nematodes: Veterinary Integrative Sciences, 20(3), 507–515. Retrieved from
Research Articles


Egyptian mango by-product 1. Compositional quality of mango seed kernel. Food Chem. 103(4), 1134-1140.

Abdel-Aty, A.M., Salama, W.H., Hamed, M.B., Fahmy, A.S., Mohamed, S.A., 2018. Phenolic-antioxidant capacity of mango seed kernels: therapeutic effect against viper venoms. Rev. Bras. Farmacogn. 28(5), 594-601.

Alawneh, J.I., Barnes, T.S., Parke, C., Lapuz, E., David, E., Basinang, V., Baluyut, A., Villar, E., Lopez, E.L., Blackall, P.J., 2014. Description of the pig production systems, biosecurity practices and herd health providers in two provinces with high swine density in the Philippines. Prev. Vet. Med. 114(2), 73-87.

Ballesteros-Vivas, D., Alvarez-Rivera, G., García Ocampo, A.F., Morantes, S.J., del Pilar Sánchez Camargo, A., Cifuentes, A., Parada-Alfonso, F., Ibánez, E., 2019. Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells. J. Supercrit. Fluids. 152, 104563.

Barrau, E., Fabre, N., Fouraste, I., Hoste, H., 2005. Effect of bioactive compounds from Sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. Parasitology. 131(4), 531-538.

Brunet, S., Hoste, H., 2006. Monomers of Condensed Tannins Affect the Larval Exsheathment of Parasitic Nematodes of Ruminants. J. Agric. Food Chem. 54(20), 7481-7487.

da Silva, G.D., de Lima, H.G., de Sousa, N.B., de Jesus Genipapeiro, I.L., Uzêda, R.S., Branco, A., Costa, S.L., Batatinha, M.J.M., Botura, M.B., 2021. In vitro anthelmintic evaluation of three alkaloids against gastrointestinal nematodes of goats. Vet. Parasitol. 296, 109505.

Dorta, E., González, M., Lobo, M.G., Laich, F., 2016. Antifungal activity of mango peel and seed extracts against clinically pathogenic and food spoilage yeasts. Nat. Prod. Res. 30(22), 2598-2604.

Engström, M., Karonen, M., Ahern, J., Baert, N., Payré, B., Hoste, H., Salminen, J.P., 2016. Chemical Structures of Plant Hydrolyzable Tannins Reveal Their in Vitro Activity against Egg Hatching and Motility of Haemonchus contortus Nematodes. J. Agric. Food Chem. 64(4), 840-851.

Hutchinson, G., 2009. Nematode parasites of small ruminants, camelids and cattle diagnosis with emphasis on anthelmintic efficacy and resistance testing. Australia and New Zealand Standard Diagnostic Procedures, Canberra, pp. 17-19.

Katiki, L.M., Ferreira, J.F., Gonzalez, J.M., Zajac, A.M., Lindsay, D.S., Chagas, A.S., Amarante, A.F., 2013. Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity. Vet. Parasitol. 192(1-3), 218-27.

Kaur, J., Rathinam, X., Kasi, M., Leng, K., Ayyalu, R., Kathiresan, S., Subramaniam, S., 2010. Preliminary investigation on the antibacterial activity of mango (Mangifera indica L: Anacardiaceae) seed kernel. Asian Pac. J. Trop. Med. 3(9), 707-710.

Kiuchi, F., Nakamura, N., Tsuda, Y., Kondo, K., Yoshimura, H., 1988. Studies on crude drugs effective on visceral larva migrans. IV. Isolation and identification of larvicidal principles in pepper. Chem. Pharm. Bull. (Tokyo). 36(7), 2452-2465.

Lee, J.A., Lañada E.B., More, S.J., Cotiw-an, B.S., Taveros, A.A., 2005. A longitudinal study of growing pigs raised by smallholder farmers in the Philippines. Prev. Vet. Med. 70(1-2), 75-93.

Luo, F., Fu,Y., Xiang, Y., Yan, S., Hu, G., Huang, X., Huang, G., Sun, C., Li, X., Chen, K., 2014. Identification and quantification of gallotannins in mango (Mangifera indica L.) kernel and peel and their antiproliferative activities. J. Funct. Foods. 8, 282-291.

Maisuthisakul, P., Gordon, M.H., 2009. Antioxidant and tyrosinase inhibitory activity of mango seed kernel by product. Food Chem. 117(2), 332-341.

Maqbool, A., Hayat, C., Tanveer, A., 2004. Comparative efficacy of various indigenous and allopathic drugs against fascioliasis in buffaloes. Vet. Arh. 74(2), 107-114.

Min, B., Hernandez, K., Pinchak, W., Anderson, R., Miller, J., Valencia, E., 2015. Effects of Plant Tannin Extracts Supplementation on Animal Performance and Gastrointestinal Parasites Infestation in Steers Grazing Winter Wheat. Open J. Anim. Sci. 5(3), 343-350.

Mukai, D., Matsuda, N., Yoshioka, Y., Sato, M., Yamasaki, T., 2008. Potential anthelmintics: polyphenols from the tea plant Camellia sinensis L. are lethally toxic to Caenorhabditis elegans. J. Nat. Med. 62, 155-159.

Nery, P.S., Nogueira, F.A., Oliveira, N.J., Martins, E.R., Duarte, E.R., 2012. Efficacy of extracts of immature mango on ovine gastrointestinal nematodes. Parasitol. Res. 111(6), 2467-2471.

Paller, V.G.V., Babia-Abion, S., 2019. Soil-transmitted helminth (STH) eggs contaminating soils in selected organic and conventional farms in the Philippines. Parasite Epidemiol. Control. 7, e00119.

Pettersson, E., Halvarsson, P., Sjölund, M., Grandi, G., Wallgren, P., Höglund, J., 2021. First report on reduced efficacy of ivermectin on Oesophagostomum spp. on Swedish pig farms. Vet. Parasitol. Reg. Stud. Reports. 25, 100598.

Ribeiro, S., Barbosa, L., Queiroz, J., Knödler, M., Schieber, A., 2008. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem. 110(3), 620-626.

Roepstorff, A., Mejer, H., Nejsum, P., Thamsborg, S.M., 2011. Helminth parasites in pigs: New challenges in pig production and current research highlights. Vet. Parasitol. 180(1-2), 72-81.

Sáyago-Ayerdi, S.G., Moreno-Hernández, C.L., Montalvo-González, E., García-Magaña, M.L., Mata-Montes de Oca, M., Torres, J.L., Pérez-Jiménez, J., 2013. Mexican ‘Ataulfo’ mango (Mangifera indica L) as a source of hydrolyzable tannins. Analysis by MALDI-TOF/TOF MS. Food Res. Int. 51(1), 188-194.

Spiegler, V., Liebau, E., Hensel, A., 2017. Medicinal plant extracts and plant-derived polyphenols with anthelmintic activity against intestinal nematodes. Nat. Prod. Rep. 34(6), 627-643.

Tomás-Barberan, F.A., Espín, J.C., García-Conesa, M.T., 2009. Bioavailability and Metabolism of Ellagic Acid and Ellagitannins. In: Quideau, S. (ed). Chemistry and Biology of Ellagitannins, World Scientific Publishing Co. Pte. Ltd., Singapore, pp. 273-297.

Torres-León, C., de Azevedo Ramos, B., dos Santos Correia, M.T., Carneiro-da-Cunha, M.G., Ramirez-Guzman, N., Alves, L.C., Brayner, F.A., Ascasio-Valdes, J., Álvarez-Pérez, O.B., Aguilar, C.N., 2021. Antioxidant and anti-staphylococcal activity of polyphenolic-rich extracts from Ataulfo mango seed. LWT. 148, 111653.

Villar, E., Maleon, M., Facundo, D., 2002. The Swine Industry in the Philippines: A Focus on Smallholders. In: Priorities for Pig Research in Southeast Asia and the Pacific to 2010. Australian Centre for International Agricultural Research, Canberra, pp. 48-56.

Yakubu, M.T., Salimon, S.S., 2015. Antidiarrhoeal activity of aqueous extract of Mangifera indica L. leaves in female albino rats. J. Ethnopharmacol. 163, 135-141.