Bactericidal activity of Piper betle L. extract against antibiotic resistant Salmonella spp. isolated from pig farms in Southern Thailand https://doi.org/10.12982/VIS.2022.042

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Published: Aug 1, 2022
Keywords:
Antibacterial activity Antibiotic resistance Pig farms Piper betle Salmonella spp

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Ratchadaporn Boripun
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand
Panida Paopradit
Faculty of Agriculture, Princess of Naradhiwas University, Narathiwas 96000, Thailand
Jindarha Prampramote
School of Agricultural Technology and food Industry Walailak University, Nakhon Si Thammarat 80160, Thailand
Ruethai Narinthorn
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand
Sutsiree Intongead
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand
Suthinee Sangkanu
School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team) and World Union for Herbal Drug Discovery (WUHeDD), Walailak University, Nakhon Si Thammarat 80160, Thailand
Tanapat Narongrit
Division of Biological Science, Faculty of sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
Watcharapong Mitsuwan
Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand Center of Excellence in Innovation of Essential Oil, Walailak University, Nakhon Si Thammarat 80160, Thailand

Abstract

Piper betle L. leaves have traditionally been used to treat various infectious diseases and to possess a wide spectrum of pharmacological effects. This study aimed to determine the antibacterial activity of the Piper betle leaf extract against antibiotic resistant Salmonella spp. isolated from pig farms located in Southern Thailand. Of this, 12 Salmonella spp. isolates were isolated from 24 pig fecal samples from 24 pig farms. The isolates were resistant to ampicillin (91.67%), penicillin (91.67%), tetracycline (81.81%), and doxycycline (81.81%). Antibacterial activity of the Piper betle ethanolic leaf extract against Salmonella spp. was carried out by disc diffusion assays, followed by Minimal inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) determination, as well as Time kill study. Piper betle extract exhibited antibacterial activity against all the isolates and S. Typhimurium with the inhibition zone ranged from 15.11 ± 0.34 to 20.30 ± 0.50 mm as observed by disc diffusion assay. The extract showed bactericidal activity against the isolates with the MIC and MBC values ranging from 0.5-1.0 mg/mL. Furthermore, the extract at 4 × MIC showed the killing activity with the reduction of the pathogen at least 3 logs within 8 h. The information suggests potential medicinal benefits of the Piper betle leaf extract to inhibit the growth of antibiotic resistant Salmonella spp. isolated from pig farms. 

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How to Cite
Boripun, R., Paopradit, P. ., Prampramote, J. ., Narinthorn, R. ., Intongead, S. ., Sangkanu, S. ., Narongrit, T. ., & Mitsuwan, W. . (2022). Bactericidal activity of Piper betle L. extract against antibiotic resistant Salmonella spp. isolated from pig farms in Southern Thailand: https://doi.org/10.12982/VIS.2022.042. Veterinary Integrative Sciences, 20(3), 557–569. Retrieved from https://he02.tci-thaijo.org/index.php/vis/article/view/258808
Issue
Vol. 20 No. 3 (2022): September-December
Section
Research Articles
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